1 research outputs found
Antimicrobial Properties and Osteogenicity of Vancomycin-Loaded Synthetic Scaffolds Obtained by Supercritical Foaming
Advanced porous synthetic
scaffolds are particularly suitable for regeneration of damaged tissues,
but there is the risk of infections due to the colonization of microorganisms,
forming biofilms. Supercritical foaming is an attractive processing
method to prepare bone scaffolds, regulating simultaneously the porosity
and loading of bioactive compounds without loss of activity. In this
work, scaffolds made of poly-ε-caprolactone (50 kDa), containing
chitosan and an antimicrobial agent (vancomycin), were processed by
supercritical CO<sub>2</sub> foaming for bone regeneration purposes.
The obtained scaffolds showed a suitable combination of morphological
(porosity, pore size distribution, and interconnectivity), time-dependent
in vitro vancomycin release behavior and biological properties (cell
viability and proliferation, osteodifferentiation, and tissue-scaffold
integration). The scaffolds sustained vancomycin release for more
than 2 weeks. Finally, the antimicrobial activity of the scaffolds
was tested against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria after 24 h of incubation with full growth inhibition for S. aureus