Stingless bee honey improves spatial memory in mice, probably associated with brain-derived neurotrophic factor (BDNF) and Inositol 1,4,5- triphosphate receptor type 1 (Itpr1) genes

This study was conducted to evaluate the effects of stingless bee honey (SBH) supplementation on memory and learning in mice. Despite many studies that show the benefits of honey on memory, reports on the nootropic effects of SBH are still lacking, and their underlying mechanism is still unclear. SBH is a honey produced by the bees in the tribe of Meliponini that exist in tropical countries. It features unique storage of honey collected in cerumen pots made of propolis. This SBH may offer a better prospect for therapeutic performance as the previous report identifies the presence of antioxidants that were greater than other honey produced by Apis sp. In this study, SBH was tested on Swiss albino mice following acute (7 days) and semichronic (35 days) supplementation. Experiments were then conducted using Morris water maze (MWM) behaviour analysis, RT-PCR for gene expression of mice striatum, and NMR for metabolomics analysis of the honey. Results indicate spatial working memory and spatial reference memory of mice were significantly improved in the honey-treated group compared with the control group. Improved memory consolidations were also observed in prolonged supplementation. Gene expression analyses of acutely treated mice demonstrated significant upregulation of BDNF and Itpr 1 genes that involve in synaptic function. NMR analysis also identified phenylalanine, an essential precursor for tyrosine that plays a role at the BDNF receptor. In conclusion, SBH supplementation for seven days at 2000 mg/kg, which is equivalent to a human dose of 162 mg/kg, showed strong capabilities to improve spatial working memory. And prolonged intake up to 35 days increased spatial reference memory in the mice model. The phenylalanine in SBH may have triggered the upregulation of BDNF genes in honey-treated mice and improved their spatial memory performance

Effects of honey produced by stingless bee (heterotrigona itama) on spatial memory and learning in female albino mice

In the 21st century, memory and learning decline is one of the most important health care issues. The use of the effect of honey on memory and learning were investigated since the last decade. To date, sources of honey are limited in tropical countries therefore, stingless bee honey is proposed as an alternative. Stingless bees are found in most subtropical and tropical regions of the world. They are highly eusocial bees and are identified with over 500 species worldwide Scientific research on specific positive effects of stingless bee honey on learning and memory are still lacking. Hence, this study intends to evaluate the effects of stingless honey bee (Heterotrigona itama) honey on memory in female albino mice. Female albino mice aged two to three months were divided into four groups with each group treated with different concentration of honey (750 mg/kg and 2000 mg/kg) for a duration of 7 and 35 days respectively and a control group. Open field exploration test was used to access spatial memory. Following behavioral tests, the mice was sacrificed, and the striatum was harvested for hematoxylin and eosin (H&E) staining. Lastly, genes linked with memory were tested through reverse transcriptase polymerase chain reaction (RT-PCR). The current findings indicated that there are changes in the dose-dependent manner (p≤ 0.05). From behavioral analysis, 2000 mg/kg of stingless honey bee supplementation for seven days showed improvement in the memory and learning of female mice compared to 750 mg/kg of stingless bee honey during total entries to the home base. However, when compared to the total time outside the home based, mice treated for a duration of 35 days spent less time outside the home base as compared to a duration of seven days indicating that mice treated for a longer duration adapted quickly to the environment thus showed a reduce in anxiety as compared to mice treated with stingless bee supplementation for a shorter duration of seven days. Stingless honey bee supplementation did not cause any neurodegenerative changes in the striatum and there was an upregulation of Bdnf, Map2k3 and Itpr1 genes from the brain derived neurotropic (BDNF) pathway. Collectively, the data in this study provides preliminary evidence suggesting that stingless bee honey (Heterotrigona itama) treatment enhances spatial memory and learning performances in female albino mice

Utilization of Emergency Department Services by Homeless Individuals in Pomona, California: A Quantitative Study

The purpose of this study was to examine the utilization of emergency department services by homeless adults in Pomona, California achieved through quantitative cross-sectional research. The results revealed that many homeless adults frequent the emergency department for mental health reasons and those homeless adults with physical ailments required longer lengths of stay and extensive discharge planning. Most participants had insurance coverage, but many appeared to lack social or primary care resources in the community, leading to frequent emergency department usage. This study may be beneficial to individuals, communities, and professionals to better understand the healthcare experiences of homeless individuals. The tangible and nontangible barriers to healthcare, as well as outside social resources affect the general public health through risks of contagious diseases and the rise in emergency department overcrowding

Caracteristicas Socio-Familiares De Los Niños Y Niñas Con Discapacidad Auditiva En Las Instituciones Educativas Rafael Uribe Uribe, San Isidoro Y Mariano Sánchez Andrade Del Municipio Del Espinal-Tolima

Documento PDFTABLA DE CONTENIDO 1. INTRODUCCION 3 2. PERFIL TECNICO 4 3. JUSTIFICACION 5 4. OBJETIVOS 6 4.1 OBJETIVO GENERAL 6 4.2 OBJETIVOS ESPECIFICOS 6 5. MARCO REFERENCIAL 7 6. MARCO CONCEPTUAL 11 7. MARCO TEORICO 17 8. MARCO INSTITUCIONAL 21 9. PROBLEMÁTICA 35 10. METODOLOGIA 41 11. RECURSOS Y PRESUPUESTO 43 12. RESULTADOS 44 13. CONCLUSIONES 55 14. RECOMENDACIONES 56 15. BIBLIOGRAFIA 57 16. ANEXOS 63PregradoTécnica(o) Profesional en Promoción Socia

Baseline Characteristics of Fabry Disease “Amenable” Migalastat Patients in Argentinian Cohort

Fabry disease (FD) is a multisystem lysosomal storage disorder induced by genetic variants in the alpha-galactosidase A (αGalA) gene. Some FD patients have GLA variants with a reduction in overall αGalA enzymatic activity due to mutated proteins with reduced stability, caused by protein misfolding and premature degradation, but the αGalA catalytic activity remains conserved (“amenable” genetic variants). To correct this misfolding and to prevent premature degradation, migalastat, a small iminosugar molecule was developed. We report the clinical characteristics of FD “amenable” cohort patients from Argentina, prior to starting treatment with migalastat. Seventeen Fabry adult patients were recruited from 13 Argentinian Centers; 8 males (47.1%) and 9 females (52.9%) were included. All genotypes included were missense-type “amenables” mutations. Some classic FD typical early manifestations were more frequent in patients with “classic” versus “late-onset” FD phenotype (pain, p=0.002; cornea verticillata, p=0.019). There was a statistically significant difference in estimated glomerular filtration rate in the “classic” versus “late-onset” phenotype (p=0.026) but no difference between genders (p=0.695). Left ventricular mass was similar between genders (p=0.145) and phenotypes (p=0.303). Cardiovascular risk factors were present among “late-onset” females (obesity 50% and smoke 25%). In patients who started “de novo” migalastat, the main indications were (i) heart disease, (ii) kidney damage, and (iii) pain, while in “switched from prior enzyme replacement therapy” patients, the most frequent indication was “patient decision;” this coincides with publications by other authors