4 research outputs found
Polarizability of Six-Helix Bundle and Triangle DNA Origami and Their Escape Characteristics from a Dielectrophoretic Trap
DNA nanoassemblies, such as DNA origamis,
hold promise in biosensing,
drug delivery, nanoelectronic circuits, and biological computing,
which require suitable methods for migration and precision positioning.
Insulator-based dielectrophoresis (iDEP) has been demonstrated as
a powerful migration and trapping tool for μm- and nm-sized
colloids as well as DNA origamis. However, little is known about the
polarizability of origami species, which is responsible for their
dielectrophoretic migration. Here, we report the experimentally determined
polarizabilities of the six-helix bundle origami (6HxB) and triangle
origami by measuring the migration times through a potential landscape
exhibiting dielectrophoretic barriers. The resulting migration times
correlate to the depth of the dielectrophoretic potential barrier
and the escape characteristics of the origami according to an adapted
Kramer’s rate model, allowing their polarizabilities to be
determined. We found that the 6HxB polarizability is larger than that
of the triangle origami, which correlates with the variations in charge
density of both origamis. Further, we discuss the orientation of both
origami species in the dielectrophoretic trap and discuss the influence
of diffusion during the escape process. Our study provides detailed
insight into the factors contributing to the migration through dielectrophoretic
potential landscapes, which can be exploited for applications with
DNA and other nanoassemblies based on dielectrophoresis
Six-Helix Bundle and Triangle DNA Origami Insulator-Based Dielectrophoresis
Self-assembled DNA nanostructures
have large potential for nanoelectronic
circuitry, targeted drug delivery, and intelligent sensing. Their
applications require suitable methods for manipulation and nanoscale
assembly as well as adequate concentration, purification, and separation
methods. Insulator-based dielectrophoresis (iDEP) provides an efficient
and matrix-free approach for manipulation of micro- and nanometer-sized
objects. In order to exploit iDEP for DNA nanoassemblies, a detailed
understanding of the underlying polarization and dielectrophoretic
migration is essential. Here, we explore the dielectrophoretic behavior
of six-helix bundle and triangle DNA origamis with identical sequence
but large topological difference and reveal a characteristic frequency
range of iDEP trapping. Moreover, the confinement of triangle origami
in the iDEP trap required larger applied electric fields. To elucidate
the observed DEP migration and trapping, we discuss polarizability
models for the two species according to their structural difference
complemented by numerical simulations, revealing a contribution of
the electrophoretic transport of the DNA origami species in the iDEP
trapping regions. The numerical model showed reasonable agreement
with experiments at lower frequency. However, the extension of the
iDEP trapping regions observed experimentally deviated considerably
at higher frequencies. Our study demonstrates for the first time that
DNA origami species can be successfully trapped and manipulated by
iDEP and reveals distinctive iDEP behavior of the two DNA origamis.
The experimentally observed trapping regimes will facilitate future
exploration of DNA origami manipulation and assembly at the nano-
and microscale as well as other applications of these nanoassemblies
with iDEP
Six-Helix Bundle and Triangle DNA Origami Insulator-Based Dielectrophoresis
Self-assembled DNA nanostructures
have large potential for nanoelectronic
circuitry, targeted drug delivery, and intelligent sensing. Their
applications require suitable methods for manipulation and nanoscale
assembly as well as adequate concentration, purification, and separation
methods. Insulator-based dielectrophoresis (iDEP) provides an efficient
and matrix-free approach for manipulation of micro- and nanometer-sized
objects. In order to exploit iDEP for DNA nanoassemblies, a detailed
understanding of the underlying polarization and dielectrophoretic
migration is essential. Here, we explore the dielectrophoretic behavior
of six-helix bundle and triangle DNA origamis with identical sequence
but large topological difference and reveal a characteristic frequency
range of iDEP trapping. Moreover, the confinement of triangle origami
in the iDEP trap required larger applied electric fields. To elucidate
the observed DEP migration and trapping, we discuss polarizability
models for the two species according to their structural difference
complemented by numerical simulations, revealing a contribution of
the electrophoretic transport of the DNA origami species in the iDEP
trapping regions. The numerical model showed reasonable agreement
with experiments at lower frequency. However, the extension of the
iDEP trapping regions observed experimentally deviated considerably
at higher frequencies. Our study demonstrates for the first time that
DNA origami species can be successfully trapped and manipulated by
iDEP and reveals distinctive iDEP behavior of the two DNA origamis.
The experimentally observed trapping regimes will facilitate future
exploration of DNA origami manipulation and assembly at the nano-
and microscale as well as other applications of these nanoassemblies
with iDEP
Six-Helix Bundle and Triangle DNA Origami Insulator-Based Dielectrophoresis
Self-assembled DNA nanostructures
have large potential for nanoelectronic
circuitry, targeted drug delivery, and intelligent sensing. Their
applications require suitable methods for manipulation and nanoscale
assembly as well as adequate concentration, purification, and separation
methods. Insulator-based dielectrophoresis (iDEP) provides an efficient
and matrix-free approach for manipulation of micro- and nanometer-sized
objects. In order to exploit iDEP for DNA nanoassemblies, a detailed
understanding of the underlying polarization and dielectrophoretic
migration is essential. Here, we explore the dielectrophoretic behavior
of six-helix bundle and triangle DNA origamis with identical sequence
but large topological difference and reveal a characteristic frequency
range of iDEP trapping. Moreover, the confinement of triangle origami
in the iDEP trap required larger applied electric fields. To elucidate
the observed DEP migration and trapping, we discuss polarizability
models for the two species according to their structural difference
complemented by numerical simulations, revealing a contribution of
the electrophoretic transport of the DNA origami species in the iDEP
trapping regions. The numerical model showed reasonable agreement
with experiments at lower frequency. However, the extension of the
iDEP trapping regions observed experimentally deviated considerably
at higher frequencies. Our study demonstrates for the first time that
DNA origami species can be successfully trapped and manipulated by
iDEP and reveals distinctive iDEP behavior of the two DNA origamis.
The experimentally observed trapping regimes will facilitate future
exploration of DNA origami manipulation and assembly at the nano-
and microscale as well as other applications of these nanoassemblies
with iDEP