4,603 research outputs found
Different laboratory mouse strains show distinct coronary artery patterns
Different laboratory mouse strains show distinct coronary artery patterns.
MC Fernández 1,2, A LĂłpez-GarcĂa 1,2, M Lorenzale 1, V Sans-Coma 1,2, AC Durán 1,2 and B Fernández 1,2.
1 Department of Animal Biology, Faculty of Science, University of Málaga, Spain.
2 Biomedical Research Institute of Málaga (IBIMA),
University of Málaga, Spain. The C57BL/6 (BL/6) mouse strain is one of the most
common models in research involving laboratory animals, particularly on studies of the cardiovascular system. It has been reported (Fernandez B, et al. J Anat 2008 212(1):12–18) that this strain presents an unusual coronary artery (CA) pattern, including congenital CA anomalies, which are clinically relevant in humans. The aim of the present study was to elucidate whether this pattern is strain-specific or appears in other mouse populations.
We used stereomicroscopy, scanning electron
microscopy, light microscopy, and a corrosion cast
technique in 597 adult mice belonging to three
inbred strains (BL/6, Balb/c, DBA/2), three outbred stocks (CD1, OF1, NMR1), two hybrid lines (129sv x BL/6, CD2F1) and wild mice.
Lock-like ostium was only detected in BL/6 mice,
whereas left septal artery, accessory ostium, high
take-off, intramural course, and solitary ostium of one CA in aorta were present in different laboratory strains and in wild mice. However, each mouse population showed a specific incidence of these coronary conditions.
These results should be taken into account when
studying the murine coronary system, especially in
CA occlusion experiments and in studies on cardiovascular development involving murine mutant lines. In addition, we propose that several laboratory mouse strains may serve as appropriate animal models to study several clinically relevant human congenital anomalies of the CAs. Our results suggest that some of these CA anomalies are subject to a simple mode of inheritance.
This work was supported by P10-CTS-6068 and PI-
0888-2012.Universidad de Málaga. Campus de Excelencia Internacional AndalucĂa Tech. P10-CTS-6068. PI-0888-201
Myocardial trabeculation in embryos of Scyliorhinus canicula (Elasmobranchii, Chondrichthyans)
Currently, three types of ventricular myoarchitecture are recognized in vertebrates, namely compact, spongy (trabeculated) and mixed myocardium. Mixed myocardium, which has been recently proposed as the primitive condition in gnathostomes, is composed of two myocardial layers: an inner trabeculated and an outer compact one. The trabeculation process has been studied in teleosts, showing exclusively spongy myocardium, and mammals and birds, characterized by a compact myocardial ventricular wall. In zebrafish, mouse and chicken embryos, the trabeculae develop as luminal myocardial ridges protruding into the lumen. In mammals and birds, further compactation of trabeculae leads to the formation of a compact layer. The potential mechanisms that may contribute to the formation of the ridges are under discussion and include myocardial proliferation, endocardial invagination, and bending of the entire myocardial layer. However, no description of the development of the mixed myocardium is available.
To shed some light on this issue, we have studied the heart development of an elasmobranch species with mixed myocardium, the lesser spotted dogfish (Scyliorhinus canicula; Chondrichthyes), by means of histological and immunohistochemical techniques for light microscopy, semithin sections, scanning electron microscopy and transmission electron microscopy.
Our results suggest that in the dogfish the intertrabecular spaces develop by connections between early intramyocardial spaces and the lumen of the ventricle through invaginations of the endocardial line. Chondrichthyans are the earliest diverged lineage of gnathostomes and, consequently, they have the most primitive cardiac design. Although chicken, mouse, and recently zebrafish have been considered powerful vertebrate models to study heart development, we propose that the trabeculation process in the dogfish is representative of the early steps of the ventricular morphogenesis in vertebrates.Universidad de Málaga. Campus de Excelencia Internacional AndalucĂa Tech.Study supported by grant CGL2017-85090-P and CGL2014-52356-P (Ministerio de EconomĂa y Competitividad), FPU15/03209 (Ministerio de EducaciĂłn, Cultura y Deporte), FEDER, and Universidad de Málaga
MYOCARDIAL STRUCTURE AND VASCULARIZATION OF THE HEART VENTRICLE IN HOLOCEPHALI: IMPLICATIONS FOR HEART EVOLUTION
El resumen aparece en el Program & Abstracts of the 10th International Congress of Vertebrate Morphology, Barcelona 2013. Anatomical Record, Volume 296, Special Feature — 1: P-075.It has been classically assumed that the ventricle of the primitive vertebrate heart is
composed of spongy myocardium, supplied exclusively by oxygen-poor, luminal blood.
This idea is on two facts: (1) extant agnathans have a spongy ventricular myocardium,
and (2) in avian and mammalian embryos, the formation of trabeculated myocardium
precedes the appearance of compact myocardium. Recently, it has been proposed that,
like elasmobranchs, the early gnathostomes possess a fully vascularised ventricle
composed of mixed myocardium. We tested this idea by studying the structure and
vascularisation of the ventricular myocardium in four holocephalan species of the
families Chimaeridae and Rhinochimaeridae. Chimaera monstrosa, Hidrolagus affinis
and Harriotta raleighana have a spongy myocardium covered by a thin layer of cardiac
muscle. In H. raleighana, the compacta is reduced to an extremely fine rim. In all three
species there is a well-developed coronary artery system consisting of subepicardial
vessels which give off branches that penetrate the myocardial trabeculae.
Rhinochimaera atlantica has no compacta and its ventricular coronary artery system is
reduced to subepicardial vessels that do not enter the spongy layer. This report is the
first to show that in wild living vertebrates, a coronary artery system supplying the whole
myocardium exists in the absence of a well-developed compacta, which supports
experimental work that shows that myocardial cell proliferation and coronary vascular
growth rely on genetically separated programs. We conclude that the mixed ventricular
myocardium is primitive for chondrichthyans, and that the lack of compacta in some
holocephalans is a derived character. Moreover our results support the hypotheses that
the mixed myocardium is the primitive condition in gnathostomes, and that the absence
of a compacta in different actinopterygian taxa is the result of its repeated loss during
evolution.Proyecto CGL2010-16417/BOS; Fondos FEDE
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