Autophagy and phagocytosis converge for better vision

The retinal pigment epithelium (RPE) is a single layer of nonregenerating cells essential to homeostasis in the retina and the preservation of vision. While the RPE perform a number of important functions, 2 essential processes are phagocytosis, which removes the most distal tips of the photoreceptors to support disk renewal, and the visual cycle, which maintains the supply of chromophore for regeneration of photo-bleached visual pigments. We recently reported that these processes are linked by a noncanonical form of autophagy termed LC3-associated phagocytosis (LAP) in which components of the autophagy pathway are co-opted by phagocytosis to recover vitamin A in support of optimal vision. Here we summarize these findings

Far-UV Emission from Elliptical Galaxies at z=0.55

The restframe UV-to-optical flux ratio, characterizing the ``UV upturn'' phenomenon, is potentially the most sensitive tracer of age in elliptical galaxies; models predict that it may change by orders of magnitude over the course of a few Gyr. In order to trace the evolution of the UV upturn as a function of redshift, we have used the far-UV camera on the Space Telescope Imaging Spectrograph to image the galaxy cluster CL0016+16 at z=0.55. Our 25''x25'' field includes four bright elliptical galaxies, spectroscopically confirmed to be passively evolving cluster members. The weak UV emission from the galaxies in our image demonstrates that the UV upturn is weaker at a lookback time 5.6 Gyr earlier than our own, as compared to measurements of the UV upturn in cluster E and S0 galaxies at z=0 and z=0.375. These images are the first with sufficient depth to demonstrate the fading of the UV upturn expected at moderate redshifts. We discuss these observations and the implications for the formation history of galaxies.Comment: 4 pages, Latex. 2 figures. Uses corrected version of emulateapj.sty and apjfonts.sty (included). Accepted for publication in ApJ Letter

Color--Luminosity Relations for the Resolved Hot Stellar Populations in the Centers of M 31 and M 32

We present Faint Object Camera (FOC) ultraviolet images of the central 14x14'' of Messier 31 and Messier 32. The hot stellar population detected in the composite UV spectra of these nearby galaxies is partially resolved into individual stars, and their individual colors and apparent magnitudes are measured. We detect 433 stars in M 31 and 138 stars in M 32, down to detection limits of m_F275W = 25.5 mag and m_F175W = 24.5 mag. We investigate the luminosity functions of the sources, their spatial distribution, their color-magnitude diagrams, and their total integrated far-UV flux. Although M 32 has a weaker UV upturn than M 31, the luminosity functions and color-magnitude diagrams of M 31 and M 32 are surprisingly similar, and are inconsistent with a majority contribution from any of the following: PAGB stars more massive than 0.56 Msun, main sequence stars, or blue stragglers. Both the the luminosity functions and color-magnitude diagrams are consistent with a dominant population of stars that have evolved from the extreme horizontal branch (EHB) along tracks with masses between 0.47 and 0.53 Msun. These stars are well below the detection limits of our images while on the zero-age EHB, but become detectable while in the more luminous (but shorter) AGB-Manque' and post-early asymptotic giant branch (PEAGB) phases. The FOC observations require that only a only a very small fraction of the main sequence population (2% in M 31 and 0.5% in M 32) in these two galaxies evolve though the EHB and post-EHB phases, with the remainder evolving through bright PAGB evolution that is so rapid that few if any stars are expected in the small field of view covered by the FOC.Comment: 35 pages, Latex. 19 figures. To appear in ApJ. Uses emulateapj.sty and apjfonts.sty (included). Color plates distributed seperatedly: fig1.jpg and fig2.jp

Validating a Vegetative Filter Strip Performance Model

Vegetative filter strips (VFS) reduce losses of nutrients, solids, and other materials from land area treated with fertilizers and manures. A number of models are available that simulate nutrient and sediment transport in VFS. While VFS effectiveness is considered to depend on lengths of pollutant source area and VFS areas, few published studies have tried to validate these models using variable pollutant source area and VFS area. The objective of this study was to validate an event-based nutrient transport model (Chaubey et al., 1995) that simulates soluble nutrient transport in VFS. This model links three sub-models: modified Green-Ampt infiltration, non-linear kinematic wave overland flow routing, and a nutrient transport component. The nutrient transport component considers infiltration as the only mechanism of pollutant removal from runoff. Data from a field plot experiment were used to validate the model. The model was executed using an uncalibrated runoff component, a calibrated runoff component, and measured runoff. The concentrations of parameters entering the VFS from three different poultry litter application lengths (6.1, 12.2, and 18.3 m) were not significantly different. However, predicted concentrations at subsequent lengths were different for all the three poultry litter application lengths. This finding was consistent with the observed data. Model execution with the uncalibrated runoff component, calibrated runoff component, and measured runoff underpredicted concentrations and mass transport at various locations along the length of the VFS. Underprediction of concentration was judged to be the reason for underprediction of mass transport. The agreement between the observed and predicted concentrations and mass transport, however, improved when runoff predictions from the calibrated runoff component and measured runoff were used. This suggests that accurate prediction of infiltration and runoff is critical for accurate prediction of concentration mass transport. Furthermore, since concentration was underpredicted even when measured runoff was used, this study suggests that the nutrient transport component might be improved, possibly by including nutrient removal mechanisms other than infiltration

Age Constraints for an M31 Globular Cluster from Main Sequence Photometry

We present a color-magnitude diagram (CMD) of the globular cluster SKHB-312 in the Andromeda galaxy (M31), obtained with the Advanced Camera for Surveys on the Hubble Space Telescope. The cluster was included in deep observations taken to measure the star formation history of the M31 halo. Overcoming a very crowded field, our photometry of SKHB-312 reaches V ~ 30.5 mag, more than 1 mag below the main sequence turnoff. These are the first observations to allow a direct age estimate from the turnoff in an old M31 cluster. We analyze its CMD and luminosity function using a finely-spaced grid of isochrones that have been calibrated using observations of Galactic clusters taken with the same camera and filters. The luminosity difference between the subgiant and horizontal branches is ~0.2 mag smaller in SKHB-312 than in the Galactic clusters 47 Tuc and NGC 5927, implying SKHB-312 is 2-3 Gyr younger. A quantitative comparison to isochrones yields an age of 10 +2.5/-1 Gyr

RR Lyrae Stars in the Andromeda Halo from Deep Imaging with the Advanced Camera for Surveys

We present a complete census of RR Lyrae stars in a halo field of the Andromeda galaxy. These deep observations, taken as part of a program to measure the star formation history in the halo, spanned a period of 41 days with sampling on a variety of time scales, enabling the identification of short and long period variables. Although the long period variables cannot be fully characterized within the time span of this program, the enormous advance in sensitivity provided by the Advanced Camera for Surveys on the Hubble Space Telescope allows accurate characterization of the RR Lyrae population in this field. We find 29 RRab stars with a mean period of 0.594 days, 25 RRc stars with a mean period of 0.316 days, and 1 RRd star with a fundamental period of 0.473 days and a first overtone period of 0.353 days. These 55 RR Lyrae stars imply a specific frequency S_RR=5.6, which is large given the high mean metallicity of the halo, but not surprising given that these stars arise from the old, metal-poor tail of the distribution. This old population in the Andromeda halo cannot be clearly placed into one of the Oosterhoff types: the ratio of RRc/RRabc stars is within the range seen in Oosterhoff II globular clusters, the mean RRab period is in the gap between Oosterhoff types, and the mean RRc period is in the range seen in Oosterhoff I globular clusters. The periods of these RR Lyraes suggest a mean metallicity of [Fe/H]=-1.6, while their brightness implies a distance modulus to Andromeda of 24.5+/-0.1, in good agreement with the Cepheid distance.Comment: 15 pages, latex. Accepted for publication in The Astronomical Journa

Measurements of the Diffuse Ultraviolet Background and the Terrestrial Airglow with the Space Telescope Imaging Spectrograph

Far-UV observations in and near the Hubble Deep Fields demonstrate that the Space Telescope Imaging Spectrograph (STIS) can potentially obtain unique and precise measurements of the diffuse far-ultraviolet background. Although STIS is not the ideal instrument for such measurements, high-resolution images allow Galactic and extragalactic objects to be masked to very faint magnitudes, thus ensuring a measurement of the truly diffuse UV signal. The programs we have analyzed were not designed for this scientific purpose, but would be sufficient to obtain a very sensitive measurement if it were not for a weak but larger-than-expected signal from airglow in the STIS 1450-1900 A bandpass. Our analysis shows that STIS far-UV crystal quartz observations taken near the limb during orbital day can detect a faint airglow signal, most likely from NI\1493, that is comparable to the dark rate and inseparable from the far-UV background. Discarding all but the night data from these datasets gives a diffuse far-ultraviolet background measurement of 501 +/- 103 ph/cm2/sec/ster/A, along a line of sight with very low Galactic neutral hydrogen column (N_HI = 1.5E20 cm-2) and extinction (E(B-V)=0.01 mag). This result is in good agreement with earlier measurements of the far-UV background, and should not include any significant contribution from airglow. We present our findings as a warning to other groups who may use the STIS far-UV camera to observe faint extended targets, and to demonstrate how this measurement may be properly obtained with STIS.Comment: 7 pages, Latex. 4 figures. Uses corrected version of emulateapj.sty and apjfonts.sty (included). Accepted for publication in A