10 research outputs found
Routine sample preparation and HPLC analysis for ascorbic acid (vitamin C) determination in wheat plants and Arabidopsis leaf tissues
Plants have developed various mechanisms to protect themselves against oxidative stress. One of the most
important non-enzymatic antioxidants is ascorbic acid. There is thus a need for a rapid, sensitive method
for the analysis of the reduced and oxidised forms of ascorbic acid in crop plants. In this paper a simple,
economic, selective, precise and stable HPLC method is presented for the detection of ascorbate in plant
tissue. The sensitivity, the short retention time and the simple isocratic elution mean that the method is
suitable for the routine quantification of ascorbate in a high daily sample number. The method has been
found to be better than previously reported methods, because of the use of an economical, readily available
mobile phase, UV detection and the lack of complicated extraction procedures. The method has been
tested on Arabidopsis plants with different ascorbate levels and on wheat plants during Cd stress