Application of General Cultural Competences in Supply Chain Management of the Business Success

Abstract This paper presents an integrated view of the literature published on all aspects and facets of competence in supply chain management (SCM) revealing that the framework brings together aspects at the individual and organizational level of competence from SCM literature. The purpose of this paper is to explore and develop the general proposition that organizational cultural compatibility between strategic supply chain partners results in improved performance outcomes for each participant in the chain. A theoretical framework is proposed of the relationship of differing organizational cultural elements (practices, values, behaviors and norms) to supply chain performance

The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis

F-BAR proteins link cellular membranes to the actin cytoskeleton in many biological processes. Here we investigated the function of the Schizosaccharomyces pombe Imp2 F-BAR domain in cytokinesis and find that it is critical for Imp2\u27s role in contractile ring constriction and disassembly. To understand mechanistically how the F-BAR domain functions, we determined its structure, elucidated how it interacts with membranes, and identified an interaction between dimers that allows helical oligomerization and membrane tubulation. Using mutations that block either membrane binding or tubulation, we find that membrane binding is required for Imp2\u27s cytokinetic function but that oligomerization and tubulation, activities often deemed central to F-BAR protein function, are dispensable. Accordingly, F-BARs that do not have the capacity to tubulate membranes functionally substitute for the Imp2 F-BAR, establishing that its major role is as a cell-cycle-regulated bridge between the membrane and Imp2 protein partners, rather than as a driver of membrane curvature

The role of Cdc14 phosphatases in the control of cell division

Abstract The periodicity of CDKs (cyclin-dependent kinases) regulates most cell cycle transitions including cytokinesis. High Cdk1 activity promotes cytoskeletal rearrangements necessary for cell division while at the same time ensuring that cytokinesis does not begin before the separation of sister chromatids during anaphase. The conserved Cdc14 (cell division cycle 14)-family of phosphatases reverses Cdk phosphorylation events and therefore Cdc14 phosphatases promote the process of cytokinesis. Here, we review the elucidated roles of Cdc14 phosphatases in cytokinesis and the current outstanding questions regarding their function in this process

A Global Census of Fission Yeast Deubiquitinating Enzyme Localization and Interaction Networks Reveals Distinct Compartmentalization Profiles and Overlapping Functions in Endocytosis and Polarity

Proteomic, localization, and enzymatic activity screens in fission yeast reveal how deubiquitinating enzyme localization and function are tuned

Using the Data-Compression Method for Studying Hunting Behavior in Small Mammals

Using the data-compression method we revealed a similarity between hunting behaviors of the common shrew, which is insectivorous, and several rodent species with different types of diet. Seven rodent species studied displayed succinct, highly predictable hunting stereotypes, in which it was easy for the data compressor to find regularities. The generalist Norway rat, with its changeable manipulation of prey and less predictable transitions between stereotype elements, significantly differs from other species. The levels of complexities of hunting stereotypes in young and adult rats are similar, and both groups had no prior experience with the prey, so one can assume that it is not learning, but rather the specificity of the organization of the stereotype that is responsible for the nature of the hunting behavior in rats. We speculate that rodents possess different types of hunting behaviors, one of which is based on a succinct insectivorous standard, and another type, perhaps characteristic of generalists, which is less ordered and is characterized by poorly predictable transitions between elements. We suggest that the data-compression method may well be more broadly applicable to behavioral analysis

Identification and Characterization of Two Novel Proteins Affecting Fission Yeast γ-tubulin Complex Function

The γ-tubulin complex, via its ability to organize microtubules, is critical for accurate chromosome segregation and cytokinesis in the fission yeast, Schizosaccharomyces pombe. To better understand its roles, we have purified the S. pombe γ-tubulin complex. Mass spectrometric analyses of the purified complex revealed known components and identified two novel proteins (i.e., Mbo1p and Gfh1p) with homology to γ-tubulin–associated proteins from other organisms. We show that both Mbo1p and Gfh1p localize to microtubule organizing centers. Although cells deleted for either mbo1(+) or gfh1(+) are viable, they exhibit a number of defects associated with altered microtubule function such as defects in cell polarity, nuclear positioning, spindle orientation, and cleavage site specification. In addition, mbo1Δ and gfh1Δ cells exhibit defects in astral microtubule formation and anchoring, suggesting that these proteins have specific roles in astral microtubule function. This study expands the known roles of γ-tubulin complex components in organizing different types of microtubule structures in S. pombe

The SIN Kinase Sid2 Regulates Cytoplasmic Retention of the S. pombe Cdc14-like Phosphatase Clp1

SummaryCdc14-family phosphatases play a conserved role in promoting mitotic exit and cytokinesis by dephosphorylating substrates of cyclin-dependent kinase (Cdk). Cdc14-family phosphatases have been best studied in yeast (for review, see [1, 2]), where budding yeast Cdc14 and its fission yeast homolog Clp1 are regulated partly by their localization; both proteins are thought to be sequestered in the nucleolus in interphase. Cdc14 and Clp1 are released from the nucleolus in mitosis, and in late mitosis conserved signaling pathways termed the mitotic exit network (MEN) and the septation initiation network (SIN) keeps Cdc14 and Clp1, respectively, out of the nucleolus through an unknown mechanism [3–6]. Here we show that the most downstream SIN component, the Ndr-family kinase Sid2, maintains Clp1 in the cytoplasm in late mitosis by phosphorylating Clp1 directly and thereby creating binding sites for the 14-3-3 protein Rad24. Mutation of the Sid2 phosphorylation sites on Clp1 disrupts the Clp1-Rad24 interaction and causes Clp1 to return prematurely to the nucleolus during cytokinesis. Loss of Clp1 from the cytoplasm in telophase renders cells sensitive to perturbation of the actomyosin ring but does not affect other Clp1 functions. Because all components of this pathway are conserved, this might be a broadly conserved mechanism for regulation of Cdc14-family phosphatases