Transgenic studies reveal the positive role of LeEIL-1 in regulating shikonin biosynthesis in Lithospermum erythrorhizon hairy roots

Time-course accumulation of shikonin in four typical hairy root lines. Value of Ei-19 or EO-13 is significantly different from that of the control line WT-1 or EV-9 at each time point from 3 to 12 days, respectively (Student’s t-test, P < 0.05). (TIF 125 kb

Additional file 6: Table S1. of Transgenic studies reveal the positive role of LeEIL-1 in regulating shikonin biosynthesis in Lithospermum erythrorhizon hairy roots

Primers for LeEIL-1 cDNA cloning and vector construction and verification. (DOC 39 kb

The First Ka-band (26.1–35 GHz) Blind Line Survey toward Orion KL

We conducted a Ka -band (26.1–35 GHz) line survey toward Orion KL using the TianMa 65 m Radio Telescope (TMRT). It is the first blind line survey in the Ka band and achieves a sensitivity at the mK level (1–3 mK at a spectral resolution of ∼1 km s ^−1 ). In total, 592 Gaussian features are extracted. Among them, 257 radio recombination lines (RRLs) are identified. The maximum Δ n of RRLs of H, He, and C are 20, 15, and 5, respectively. Through stacking, we have detected the β lines of ion RRLs (RRLs of C ^+ with the possible contribution of other ions like O ^+ ) for the first time, and a tentative signal of the γ lines of ion RRLs can also be seen on the stacked spectrum. Besides this, 318 other line features were assigned to 37 molecular species, and 10 of these species were not detected in the Q -band survey of TMRT. The vibrationally excited states of nine species were also detected. The emission of most species can be modeled under LTE. A number of transitions of E-CH3OH ( J _2 − J _1 ) display maser effects, which are confirmed by our modeling, and besides the bumping peak at J ∼ 6, there is another peak at J ∼ 13. Methylcyanoacetylene (CH _3 C _3 N) is detected in Orion KL for the first time. This work emphasizes that the Ka band, which was long ignored for spectral line surveys, is very useful for surveying RRLs and molecular lines simultaneously

First detection of radio recombination lines of ions heavier than helium

We report the first detection of radio recombination lines (RRLs) of ions heavier than helium. In a highly sensitive multi-band (12–50 GHz) line survey toward Orion KL with the TianMa 65-m Radio Telescope (TMRT), we successfully detected more than fifteen unblended α lines of RRLs of singly ionized species (XI