1 research outputs found
A Retrievable and Highly Selective Fluorescent Probe for Monitoring Sulfide and Imaging in Living Cells
A novel selective fluorescent chemosensor based on an
8-hydroxyquinoline-appended
fluorescein derivative (<b>L1</b>) was synthesized and characterized.
Once combined with Cu<sup>2+</sup>, it displayed high specificity
for sulfide anion. Among the various anions, only sulfide anion induced
the revival of fluoresecence of <b>L1</b>, which was quenched
by Cu<sup>2+</sup>, resulting in “off–on”-type
sensing of sulfide anion. What’s more, the sensor was retrievable
to indicate sulfide anions with Cu<sup>2+</sup>, and S<sup>2–</sup>, in turn, increased. With the addition of Cu<sup>2+</sup>, compound <b>L1</b> could give rise to a visible pink-to-yellow color change
and green fluorescence quenching. The resulting yellow solution could
change to pink and regenerate to green fluorescence immediately upon
the addition of sulfide anion; however, no changes were observed in
the presence of other anions, including CN<sup>–</sup>, P<sub>2</sub>O<sub>7</sub><sup>4–</sup>, and other
forms of sulfate, making compound <b>L1</b> an extremely selective
and efficient sulfide chemosensor. The signal transduction occurs
via reversible formation–separation of complex <b>L1</b>Cu and CuS. What’s more, the biological imaging study has
demonstrated that the chemosensor can detect sulfur anions in biological
systems at a relatively low concentration