1 research outputs found
Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe
Single-cell
metabolomics has the potential to reveal
unique insights
into intracellular mechanisms and biological processes. However, the
detection of metabolites from individual cells is challenging due
to their versatile chemical properties and concentrations. Here, we
demonstrate a tapered probe for pneumatically assisted nanospray desorption
electrospray ionization (PA nano-DESI) mass spectrometry that enables
both chemical imaging of larger cells and global metabolomics of smaller
15 μm cells. Additionally, by depositing cells in predefined
arrays, we show successful metabolomics from three individual INS-1
cells per minute, which enabled the acquisition of data from 479 individual
cells. Several cells were used to optimize analytical conditions,
and 93 or 97 cells were used to monitor metabolome alterations in
INS-1 cells after exposure to a low or high glucose concentration,
respectively. Our analytical approach offers insights into cellular
heterogeneity and provides valuable information about cellular processes
and responses in individual cells