Mononuclear cells from umbilical cord blood and from granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood. Analysis of proliferation and apoptosis in vitro

Células mononucleares de sangue de cordão umbilical (SCU) e sangue periférico mobilizado (SPM) com G-CSF, foram cultivadas in vitro com citocinas, na presença ou não de estroma de medula óssea. Os objetivos foram avaliar a capacidade proliferativa de células progenitoras, a ocorrência de apoptose e expressão de integrina. Nas culturas sem estroma, a celularidade aumentou 5 vezes (SCU) e não se alterou nas de SPM. O total de células CD34+ caiu em ambas culturas. Com estroma, o total de células nucleadas aumentou 7 vezes (SCU) e 2,3 vezes (SPM). O total de células CD34+ permaneceu o mesmo. A apoptose foi menor nas culturas de SCU. A expressão de integrina caiu, na população de células CD34+ e de CD45+Mononuclear cells from umbilical cord blood (UCB) and G-CSF mobilized peripheral blood (MPB), were cultured in vitro, in the presence of cytokines, with or without bone marrow stroma. The aims were to evaluate the proliferative response of progenitor cells, occurrence of apoptosis and expression of adhesion molecule. In cultures without stroma, cellularity increased 5-fold for UCB, but has not changed for MPB. The number of CD34+ cells has dropped in both culture. With stroma, total nucleated cells had a 7-fold increse (UCB) and a 2,3-fold (MBP), however, CD34+ cells number has not changed. Apoptosis was lower in UCB culture. The expression of integrin decreased, in the CD34+ and CD45+ populatio

Mononuclear cells from umbilical cord blood and from granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood. Analysis of proliferation and apoptosis in vitro

Células mononucleares de sangue de cordão umbilical (SCU) e sangue periférico mobilizado (SPM) com G-CSF, foram cultivadas in vitro com citocinas, na presença ou não de estroma de medula óssea. Os objetivos foram avaliar a capacidade proliferativa de células progenitoras, a ocorrência de apoptose e expressão de integrina. Nas culturas sem estroma, a celularidade aumentou 5 vezes (SCU) e não se alterou nas de SPM. O total de células CD34+ caiu em ambas culturas. Com estroma, o total de células nucleadas aumentou 7 vezes (SCU) e 2,3 vezes (SPM). O total de células CD34+ permaneceu o mesmo. A apoptose foi menor nas culturas de SCU. A expressão de integrina caiu, na população de células CD34+ e de CD45+Mononuclear cells from umbilical cord blood (UCB) and G-CSF mobilized peripheral blood (MPB), were cultured in vitro, in the presence of cytokines, with or without bone marrow stroma. The aims were to evaluate the proliferative response of progenitor cells, occurrence of apoptosis and expression of adhesion molecule. In cultures without stroma, cellularity increased 5-fold for UCB, but has not changed for MPB. The number of CD34+ cells has dropped in both culture. With stroma, total nucleated cells had a 7-fold increse (UCB) and a 2,3-fold (MBP), however, CD34+ cells number has not changed. Apoptosis was lower in UCB culture. The expression of integrin decreased, in the CD34+ and CD45+ populatio

Immunomodulatory effect of mesenchymal stem cells

Mesenchymal stem cells represent an adult population ofnonhematopoietic cells, which can differentiate into a variety of celltypes such as osteocytes, chondrocytes, adipocytes, and myocytes.They display immunomodulatory properties that have led to theconsideration of their use for the inhibition of immune responses. Inthis context, mesenchymal stem cells efficiently inhibit maturation,cytokine production, and the T cell stimulatory capacity of dendriticcells. They also can impair proliferation, cytokine secretion, andcytotoxic potential of T lymphocytes. Moreover, mesenchymal stem cells are able to inhibit the differentiation of B cells to plasma cells by inhibiting their capacity to produce antibodies. A variety of animal models confirm the immunomodulatory properties of mesenchymal stem cells. Clinical studies including patients withsevere acute graft-versus-host disease have revealed that theadministration of mesenchymal stem cells results in significantclinical responses. Therefore, mesenchymal stem cells improve acutegraft-versus-host disease and represent a promising candidate for theprevention and treatment of immune-mediated diseases, due to theirimmunomodulatory capability and their low immunogenicity

Isolation and characterization of mesenchymal stem cells obtained from reusable and disposable bone marrow collection filters

Objective: To compare human mesenchymal stem cells obtainedfrom reusable and disposable filters and to characterize themaccording to the criteria of the International Society of CellularTherapy. Methods: Human mesenchymal stem cells were isolatedfrom bone marrow collection reusable sets and compared with thoseobtained from disposable sets by washing the filters with cell culturemedia. The isolated cells were characterized according to the criteriaof the International Society of Cellular Therapy using flow cytometry,differentiation in vitro, and cytochemistry techniques. Results:Samples were obtained from disposable (n=3) and from reusablecollection sets (n=3). All samples obtained from bone marrowdisposable sets successfully produced mesenchymal stem cells. Allbone marrow derived mesenchymal stem cells were characterizedand fulfilled the criteria established by International Society of Cellular Therapy. Conclusion: This study showed that mesenchymal stemcells can also be obtained from reusable collection sets (which arestill used in several centers around the world) to be employed inresearch as an alternative and ethical source