Effect of Bioglass 45S5 air-abrasion on dentin bonding: evaluation of microtensile bond strength and confocal microscopy

19Made available in DSpace on 2019-09-11T20:51:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2015The aim of this study was to evaluate the influence of air-abrasion executed with Bioglass 45S5 on the bonding performance of total and self-etching techniques. Human molars were divided into four groups (n = 5) according to the surface treatment: G1: self-etching without air-abrasion; G2: total-etching without air-abrasion; G3: self-etching with air-abrasion Bioglass 45S5 for 1 min; and G4: total-etching with air-abrasion Bioglass 45S5 for 1 min. The Single Bond Universal (3 M ESPE, St. Paul, Minnesota, USA) was used in both etching techniques. The adhesive was photo-activated by a LED with 800 mW/cm2 irradiance (Ultralume 5, Ultradent Products, South Jordan, UT, USA). Composite resin blocks were made on the dentin using Filtek Z100 (3M ESPE, St. Paul, MN, USA). Then, the samples were cut to obtain sticks attached to a jig in a universal testing machine (EZ Test, Shimadzu, Kyoto, Kansai, Japan) for testing microtensile bond strength (μTBS). The values μTBS of microtensile bond strength were submitted to two-way ANOVA and means were compared by Tukey test (p < 0.05). The results showed significant differences in μTBS between G1 and G2. No differences were found between G3 and G4. For the self-etching technique no differences were found for G1 and G3. In total-etching, G2 showed greater μTBS than G4. The application of Bioglass 45S5 on dentin did not increase the microtensile bond strength in self-etching groups and decreased the bond strength values for total-etching groups, but improved the quality of the hybrid layer. © 2015, Sinhoreti et al. All Right Reserved.Sinhoreti, M.A.C., Department of Operative Dentistry, Piracicaba Dental School, University of Campinas, Av. Limeira 901, Piracicaba, SP 13414-903, BrazilVitti, R.P., Department of Prosthodontics, School of Dentistry, University of Taubaté, 09 Rua Dos Operários, Taubaté, SP 12020-270, BrazilAbuna, G., Department of Operative Dentistry, Piracicaba Dental School, University of Campinas, Av. Limeira 901, Piracicaba, SP 13414-903, BrazilFeitosa, V.P., Department of Operative, School of Dentistry, Federal University of Ceará, Fortaleza, Rua Alexandre Baraúna 949, Fortaleza, CE 60430-160, Brazi

Frequência de anticorpos contra o vírus da língua azul em ovinos do estado do Ceará, Brasil

O objetivo deste trabalho foi avaliar a ocorrência de ovinos soropositivos para o vírus da língua azul (VLA) no Estado do Ceará, Brasil, e analisar as proteínas imunogênicas das cepas virais circulantes nesses rebanhos. O teste de imunodifusão em gel de agarose (IDGA) foi utilizado para pesquisar 271 amostras de soro oriundas de 16 rebanhos. Os resultados demonstraram que 27,3% (74/271) das amostras analisadas apresentaram anticorpos contra o agente e 68,8% (11/16) das propriedades tiveram animais positivos. O immunoblotting (IB) foi utilizado para analisar as proteínas imunogênicas do VLA a partir dos soros de animais positivos no IDGA. Os soros demonstraram forte reação contra a proteína viral VP2. Para o VLA, das sete proteínas estruturais, a VP2 é a principal a estimular a resposta imune protetora. Concluiu-se que a soropositividade para a língua azul (LA) nos rebanhos ovinos estudados no Ceará é alta, apesar dos animais não apresentarem sinais clínicos, indicativo de que o vírus ocorra de forma endêmica. Além disso, a resistência à doença apresentada pelos animais pode estar relacionada com a forte reação imunológica desses à proteína VP2. Sendo assim, outros estudos são necessários para melhor esclarecer a situação epidemiológica da LA no país, através da identificação dos vetores e sorotipos virais circulantes nas diferentes regiões.The objective of this work was to verify the occurrence of sheep serologically positive for bluetongue virus (BTV) in the state of Ceará, Brazil, and analyze immunogenic proteins of circulating viral strains in these flocks. The agar gel immunodifusion test (AGID) was used to examine 271 serum samples from 16 herds. The results demonstrated that 27.3% (74/271) of the analyzed samples presented antibodies for the agent, and that 68.8% (11/16) of the properties presented positive animals. Immunoblotting (IB) was used to analyze the immunogenic proteins of BTV derived from AGID positive sera. Sera showed strong reaction against viral protein VP2. of the seven BTV structural proteins, VP2 is the major protein to elicit protective immune responses. It was concluded that bluetongue (BT) seropositivity in sheep flocks studied in Ceará is high, despite that the animal's do not show clinical signs, indicating that it occurs in an endemic form. The animals' resistance to the disease may be related to the strong immune response to the protein VP2. Therefore, further studies are needed to better clarify the epidemiological situation of BT in Brazilian sheep flocks, through the identification of viral vectors and serotypes circulating in different regions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES