Melt curves of the GAPDH amplicon in the presence of EG (solid line) or SG (dashed line) following the amplification of 100 pg human cDNA input as described for Figure 9

<p><b>Copyright information:</b></p><p>Taken from "Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications"</p><p>http://www.biomedcentral.com/1472-6750/7/76</p><p>BMC Biotechnology 2007;7():76-76.</p><p>Published online 9 Nov 2007</p><p>PMCID:PMC2213645.</p><p></p> The melt peak recorded with EG is about 10 times more intense than that recorded with SG while its width is only about one half of the latter at the half-peak height

Amplification plots (Panel A) of GAPDH from various input of cDNA using either EG (open circle) or SG (solid triangle) as the DNA-binding dye in the PCR mix

<p><b>Copyright information:</b></p><p>Taken from "Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications"</p><p>http://www.biomedcentral.com/1472-6750/7/76</p><p>BMC Biotechnology 2007;7():76-76.</p><p>Published online 9 Nov 2007</p><p>PMCID:PMC2213645.</p><p></p> All reactions were run in duplicates, but only one plot per duplicate is shown for clarity. Plots of Ct value vs. logarithm cDNA input amount for both sets of reactions are shown in panel B. Reactions with EG (open circle) showed earlier Ct values and high amplification efficiency

Effect of chain extension time on qPCR using EG or SG: Amplification plots of McG (Panel A1, and A2), TBP (Panel B1 and B2) and GCL (Panel C1 and C2) with EG or SG at 0

<p><b>Copyright information:</b></p><p>Taken from "Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications"</p><p>http://www.biomedcentral.com/1472-6750/7/76</p><p>BMC Biotechnology 2007;7():76-76.</p><p>Published online 9 Nov 2007</p><p>PMCID:PMC2213645.</p><p></p>5× or 1× dye concentration: 0.5 × EG (dark lines E1–E4 in panels A1, B1 and C1); 1 × EG (dark lines E5–E8 in panels A2, B2 and C2); 0.5 × SG (gray lines S1–S4 in panels A1, B1 and C1); 1 × SG (gray lines S5–S8 in panels A2, B2 and C2). Four different elongation times, 60 seconds (lines E1, E5, S1 and S5), 30 seconds (lines E2, E6, S2 and S6), 15 seconds (lines E3, E7, S3 and S7) and 5 seconds (lines E4, E8, S4 and S8), were used for the amplification of each gene fragment with each dye and dye concentration. Line trace numbers within parenthesis indicate formation of nonspecific products as confirmed by post PCR DNA melt curve analysis

Absorption spectra of EG in the presence of various amounts of λDNA: 0 ng/μL (dotted line); 5 ng/μL (line 1), 10 ng/μL (line 2), 25 ng/μL (line 3) and 100 ng/μL (line 4)

<p><b>Copyright information:</b></p><p>Taken from "Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications"</p><p>http://www.biomedcentral.com/1472-6750/7/76</p><p>BMC Biotechnology 2007;7():76-76.</p><p>Published online 9 Nov 2007</p><p>PMCID:PMC2213645.</p><p></p> All spectra were measured at room temperature in 100 mM Tris buffer, pH 8.0 with an EG concentration of 11.15 μM. The arrows indicate trends of absorbance change in response to the increase of DNA amount. All absorbance points are relative to the maximum of line 4

Stability comparison between EvaGreen (open circle) and SYBR Green I (filled square)

<p><b>Copyright information:</b></p><p>Taken from "Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications"</p><p>http://www.biomedcentral.com/1472-6750/7/76</p><p>BMC Biotechnology 2007;7():76-76.</p><p>Published online 9 Nov 2007</p><p>PMCID:PMC2213645.</p><p></p> EvaGreen (11.42 μM) and SYBR Green I (12.83 μM) were each incubated in 100 mM Tris pH 8.0 at 99°C and sampled at time 0, 0.5, 1 and 3 hours for absorption spectrum measurement at room temperature. ROX was added to each solution to give an absorbance of 0.17 at 580 nm and was used as a stable reference to correct for spectral baseline shift due to either solvent evaporation or instrument instability. The normalized peak intensities of each dye in absorbance at each time points (relative to 0 hour) were plotted against the hours the dye stayed at 99°C

Band structure for pure and W-doped anatase TiO₂.

<p>(A) pure anatase TiO₂ unit cell, (B) Ti_0.97917W_0.02083O₂ supercell, (C) Ti_0.96875W_0.03125O₂ supercell, (D) Ti_0.95833W_0.04167O₂ supercell.</p

Optical absorption curves for pure and W-doped anatase TiO₂ under the condition of spin.

<p>Optical absorption curves for pure and W-doped anatase TiO₂ under the condition of spin.</p

Lattice parameters, total energies, and formation energies of pure and W-doped anatase TiO_2.

<p>Lattice parameters, total energies, and formation energies of pure and W-doped anatase TiO_2.</p

Theoretical models.

<p>(A) pure anatase TiO₂ unit cell, (B) Ti_0.97917W_0.02083O₂ supercell, (C) Ti_0.96875W_0.03125O₂ supercell, (D) Ti_0.95833W_0.04167O₂ supercell, (F) Ti_0.9375W_0.0625O₂ supercell.</p

Mulliken bond population and bond length of non-spin Ti_0.95833W_0.04167O₂ and Ti_0.9375 W_0.0625O₂.

<p>Mulliken bond population and bond length of non-spin Ti_0.95833W_0.04167O₂ and Ti_0.9375 W_0.0625O₂.</p