SORPTION РATHOGENETIC THERAPY OF ENDOGENOUS INTOXICATION OF PEDIATRIC CHRONIC INFLAMMATORY BOWEL DISEASES

Gut endotoxicosis caused by penetration of bacterial and metabolic toxins from chime on the background of increasing permeability of gut wall is of great importance in pathogenesis of chronic inflammatory bowel diseases (nonspecific ulcerative colitis — NUC and Crohn’s disease). It is accompanied by disturbance of regulating homeostasis system with the following disturbances of organs and systems of toxication. Developed endotoxicosis accordingly contributes to maintain and to progress of metabolic and immunological changes. To obtain the precise degree and phase of development of endotoxicosis we estimated quantitative and qualitative changes of metabolic status in accordance with content in erythrocytes, plasma and urine LMMWP (low and medium molecular weight peptides). Taking into concideration the peculiarities of children endotoxicosis with, we suggested patogenetical absorption therapy. Therefore, the therapeutic complex was added enterosorbent ensoral, which absorb eczo and endogenic toxins and, moreover, positive influence for composition of intestinal microflora. Prominent clinical effect was accompanied by positive dynamics of laboratory-instrumental parameters.Key words: endogenous intoxication, inflammatory bowel diseases, nonspecific ulcerative colitis, Crohn’s disease, low and medium molecular weight peptides, enterosorbents, children

SORPTION РATHOGENETIC THERAPY OF ENDOGENOUS INTOXICATION OF PEDIATRIC CHRONIC INFLAMMATORY BOWEL DISEASES

Gut endotoxicosis caused by penetration of bacterial and metabolic toxins from chime on the background of increasing permeability of gut wall is of great importance in pathogenesis of chronic inflammatory bowel diseases (nonspecific ulcerative colitis — NUC and Crohn’s disease). It is accompanied by disturbance of regulating homeostasis system with the following disturbances of organs and systems of toxication. Developed endotoxicosis accordingly contributes to maintain and to progress of metabolic and immunological changes. To obtain the precise degree and phase of development of endotoxicosis we estimated quantitative and qualitative changes of metabolic status in accordance with content in erythrocytes, plasma and urine LMMWP (low and medium molecular weight peptides). Taking into concideration the peculiarities of children endotoxicosis with, we suggested patogenetical absorption therapy. Therefore, the therapeutic complex was added enterosorbent ensoral, which absorb eczo and endogenic toxins and, moreover, positive influence for composition of intestinal microflora. Prominent clinical effect was accompanied by positive dynamics of laboratory-instrumental parameters.Key words: endogenous intoxication, inflammatory bowel diseases, nonspecific ulcerative colitis, Crohn’s disease, low and medium molecular weight peptides, enterosorbents, children

ИСПОЛЬЗОВАНИЕ МАТЕМАТИЧЕСКОЙ МОДЕЛИ ИНФРАКРАСНОЙ СПЕКТРОСКОПИИ СЫВОРОТКИ КРОВИ В ДИФФЕРЕНЦИАЛЬНОЙ ДИАГНОСТИКЕ ЯЗВЕННОГО КОЛИТА И БОЛЕЗНИ КРОНА У ДЕТЕЙ

Relevance of research. Inflammatory bowel diseases are among the most severe pathologies in pediatric gastroenterology, often lead to disability. Despite the similarity of the clinic, pathogenetic mechanisms, the question of the differential diagnosis of ulcerative colitis and Crohn's disease is relevant in view of their different prognosis and treatment strategy. In recent years, in medical uses infrared spectroscopy of blood serum for the differential diagnosis of various inflammatory diseases, benign and malignant tumors. Besides finding increasing application of mathematical methods for data processing, the so-called mathematical modeling of pathological processes, allowing objectify the survey results for a more accurate diagnosis and prognosis of pathological processes. Objective: improving the differential diagnosis of ulcerative colitis and Crohn's disease in children. Patients: 21 children with ulcerative colitis, 56 children with Crohn's disease and 34 healthy children. The method of infrared spectroscopy of serum and mathematical modeling results through multivariate entropy analysis. Results: the obtained spectral characteristics of blood serum in children with ulcerative colitis and Crohn's disease and in healthy children, as well as "images of disease" in these pathologies. Conclusion: The use of this medical technology reduced the time of diagnosis, which contributes to the timely choice of rational treatment strategies and provides an opportunity to avoid the development of complications, worsening of the disease. Актуальность. Воспалительные заболевания кишечника относятся к одним из самых тяжелых патологий в детской гастроэнтерологии, часто приводящих к инвалидизации. Несмотря на схожесть клиники, патогенетических механизмов, вопрос дифференциальной  диагностики язвенного колита и болезни Крона актуален ввиду их различного прогноза и лечебной тактики. В медицине в последние годы используется инфракрасная спектроскопия сыворотки крови для проведения дифференциальной диагностики различных воспалительных заболеваний, доброкачественных и злокачественных новообразований. Кроме того находят все большее применение методы математической обработки данных, так называемое математическое моделирование патологических процессов, позволяющее объективизировать результаты обследования для более точной диагностики и прогнозирования патологических процессов. Цель исследования: совершенствование дифференциальной диагностики язвенного колита и болезни Крона у детей. Пациенты и методы: 21 ребенок с язвенным колитом, 56 детей с болезнью Крона и 34 практически здоровых ребенка. Использован метод инфракрасной спектроскопии сыворотки крови и математического моделирования результатов путем многомерного энтропийного анализа. Результаты: получены спектральные характеристики сыворотки крови у детей с язвенным колитом и болезнью Крона и у здоровых детей, а также «образы болезни» при этих патологиях. Заключение: использование данной медицинской технологии позволяет сократить сроки постановки диагноза, что способствует своевременному выбору рациональной тактики лечения и дает возможность избежать развития осложнений, утяжеления течения заболевания.

Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity

The influence of protein-peptide compounds, extracted with deuterium water as solubilizer agent from mixture of Sus scofa thymus, spleen and lymph nodes extracts, was carried out on Wistar rats with cytostatic-induced immunodeficiency model. Intragastric administration of extract fraction with molecular weight more than 30 kDa, did not lead to significant changes. Thus, polypeptide compounds with molecular weight less than 30 kD, extracted from Sus scofa immune organs, significantly increased T-lymphocytes amount, affected macrophages system by T-helper (CD4) stimulation and activated cycle of complementary cascade. Differences between the experimental results in T-helper synthesis activation (CD4) in animals treated with the medium and low molecular weight fractions can be explained by the ability of biomolecules having a molecular weight less than 30 kDa (in particular, isolated from the spleen, which, in its cellular structure contains key regulatory factors) to change the speed of cell differentiation

Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity

The influence of protein-peptide compounds, extracted with deuterium water as solubilizer agent from mixture of Sus scofa thymus, spleen and lymph nodes extracts, was carried out on Wistar rats with cytostatic-induced immunodeficiency model. Intragastric administration of extract fraction with molecular weight more than 30 kDa, did not lead to significant changes. Thus, polypeptide compounds with molecular weight less than 30 kD, extracted from Sus scofa immune organs, significantly increased T-lymphocytes amount, affected macrophages system by T-helper (CD4) stimulation and activated cycle of complementary cascade. Differences between the experimental results in T-helper synthesis activation (CD4) in animals treated with the medium and low molecular weight fractions can be explained by the ability of biomolecules having a molecular weight less than 30 kDa (in particular, isolated from the spleen, which, in its cellular structure contains key regulatory factors) to change the speed of cell differentiation

ПАТОГЕНЕТИЧЕСКАЯ СОРБЦИОННАЯ ТЕРАПИЯ ЭНДОГЕННОЙ ИНТОКСИКАЦИИ ВОСПАЛИТЕЛЬНЫХ ЗАБОЛЕВАНИЙ КИШЕЧНИКА У ДЕТЕЙ

Gut endotoxicosis caused by penetration of bacterial and metabolic toxins from chime on the background of increasing permeability of gut wall is of great importance in pathogenesis of chronic inflammatory bowel diseases (nonspecific ulcerative colitis — NUC and Crohn’s disease). It is accompanied by disturbance of regulating homeostasis system with the following disturbances of organs and systems of toxication. Developed endotoxicosis accordingly contributes to maintain and to progress of metabolic and immunological changes. To obtain the precise degree and phase of development of endotoxicosis we estimated quantitative and qualitative changes of metabolic status in accordance with content in erythrocytes, plasma and urine LMMWP (low and medium molecular weight peptides). Taking into concideration the peculiarities of children endotoxicosis with, we suggested patogenetical absorption therapy. Therefore, the therapeutic complex was added enterosorbent ensoral, which absorb eczo and endogenic toxins and, moreover, positive influence for composition of intestinal microflora. Prominent clinical effect was accompanied by positive dynamics of laboratory-instrumental parameters.Key words: endogenous intoxication, inflammatory bowel diseases, nonspecific ulcerative colitis, Crohn’s disease, low and medium molecular weight peptides, enterosorbents, children.Возникновение эндогенной интоксикации у детей с воспалительными заболеваниями кишечника связано с повышенной проницаемостью кишечной стенки, степень которой зависит от глубины характера поражения толстой кишки. Развивающаяся в результате эндогенной интоксикации перестройка обменных процессов, перенапряжение адаптации, срыв работы компенсаторных механизмов ведут к структурно-метаболическим нарушениям и поддержанию эндотоксикоза. Для оценки выраженности эндогенной интоксикации определяли вещества низкой и средней молекулярной массы (ВНСММ) в эритроцитах, плазме и моче. В комплексную терапию хронических колитов были включены энтеросорбенты. Улучшение клинических проявлений после 2-недельного курса лечения сопровождалось улучшением показателей эндогенной интоксикации.Ключевые слова: эндогенная интоксикация, воспалительные заболевания кишечника, неспецифический язвенный колит, болезнь Крона, вещества низкой и средней молекулярной массы, энтеросорбенты, дети. (Педиатрическая фармакология. – 2009; 6(5):34-37

ДЛИТЕЛЬНАЯ ТЕРАПИЯ ИНФЛИКСИМАБОМ У ДЕВОЧКИ 14 ЛЕТ С БОЛЕЗНЬЮ КРОНА

.(Педиатрическая фармакология. – 2009;6(4):131-134)