LEÏOMYOSARCOME UTERIN DE PRESENTATION INHABITUELLE

Utérin Leiomyosarcoma is rare [1.5]. It is likely conjunctiva and developed at the expense of mesenchymal elements of the myometrium. The uterus bicornis united cervical represents 39% of all uterine malformations [8]. The prevalence of these abnormalities is estimated at 0.5%. The association leïomyosarcoma and uterine malformation still very exceptional. The purpose of our work is to study the unique combination from the clinical, paraclinical, pathological, and evolving therapeutic.Le léiomyosarcome utérin est une tumeur maligne, rare [1,5].Elle est de nature conjonctive et développée aux dépens des éléments mésenchymateux du myomètre. L’utérus bicorne uni cervical représente 39% de l’ensemble des malformations utérines [8]. La prévalence de ces malformations est estimée à 0,5%. L’association leïomyosarcome utérin et malformation utérine reste très exceptionnelle et ce du fait que la malformation utérine est de diagnostic précoce à un âge jeune contrairement au leiomyosarcome utérin qui survient chez des femmes d’âge avancé. Le but de notre travail est d’étudier à partir de notre observation cette association exceptionnelle sur le plan clinique, paraclinique, anatomopathologique, thérapeutique et évolutif

In Silico Investigation of Aristolochia longa Anticancer Potential against the Epidermal Growth Factor Receptor (EGFR) in the Tyrosine Kinase Domain

This study employed in silico methods to identify potential therapeutic targets for the inactive EGFR tyrosine kinase domain in complex with Erlotinib (PDB: 4HJO) which is known to cause cancer, using natural extracts from Aristolochia longa root. A library comprising five natural compounds (Luteolin, 4-hydroxycinnamic acid, Kaempferol, ferulic acid, citric acid, and quinic acid) and the standard Erlotinib (control) were subjected to Lipinski's rule of five, ADMET parameter analyses, molecular docking and molecular dynamics simulation. Results revealed comparable pharmacological responses between the five compounds and the standard drug, demonstrating promising outcomes without limitations. Notably, Luteolin, Kaempferol, and quinic acid exhibited higher binding energies than the reference molecule, with binding affinities of -9.083 kcal/mol, -8.260 kcal/mol, and -5.857 kcal/mol, respectively. Molecular dynamics simulations confirmed the stability of the most effective EGFR protein-ligand, displaying consistent interaction profiles, favorable molecular properties, and a stable trajectory (RMSD, RMSF). Overall, these in silico analyses highlight the potential of aromatic and medicinal plant-derived compounds to inhibit EGFR protein associated with cancer development, emphasizing the need for further in vitro and in vivo investigations to explore their therapeutic applications in cancer patients

Modulation of T-type Ca2+ channels by Lavender and Rosemary extracts

Medicinal plants represent a significant reservoir of unexplored substances for early-stage drug discovery. Of interest, two flowering Mediterranean plants have been used for thousands of years for their beneficial effects on nervous disorders, including anxiety and mood. However, the therapeutic potential of these plants regarding their ability to target ion channels and neuronal excitability remains largely unknown. Towards this goal, we have investigated the ability of Lavender and Rosemary to modulate T-type calcium channels (TTCCs). TTCCs play important roles in neuronal excitability, neuroprotection, sensory processes and sleep. These channels are also involved in epilepsy and pain. Using the whole-cell patch-clamp technique, we have characterized how Lavender and Rosemary extracts, as well as their major active compounds Linalool and Rosmarinic acid, modulate the electrophysiological properties of recombinant TTCCs (CaV3.2) expressed in HEK-293T cells. Both the methanolic and essential oil extracts as well as the active compounds of these plants inhibit Cav3.2 current in a concentration-dependent manner. In addition, these products also induce a negative shift of the steady-state inactivation of CaV3.2 current with no change in the activation properties. Taken together, our findings reveal that TTCCs are a molecular target of the Lavender and Rosemary compounds, suggesting that inhibition of TTCCs could contribute to the anxiolytic and the neuroprotective effects of these plants