Impacts of elevated atmospheric CO2 on litter quality, litter decomposability and nitrogen turnover rate of two oak species in a Mediterranean forest ecosystem

Elevated CO2 may affect litter quality of plants, and subsequently C and N cycling in terrestrial ecosystems, but changes in litter quality associated with elevated CO2 are poorly known. Abscised leaf litter of two oak species (Quercus cerris L., and Q. pubescens Willd.) exposed to long-term elevated CO2 around a natural CO2 spring in Tuscany (Italy) was used to study the impact of increasing concentration of atmospheric CO2 on litter quality and C and N turnover rates in a Mediterranean-type ecosystem. Litter samples were collected in an area with elevated CO2 (>500 ppm) and in an area with ambient CO2 concentration (360 ppm). Leaf samples were analysed for concentrations of total C, N, lignin, cellulose, acid detergent residue (ADR) and polphenol. The decomposition rate of litter was studied using a litter bag experiment (12 months) and laboratory incubations (3 months). In the laboratory incubations, N mineralization in litter samples was measured as well (125 days). Litter quality was expressed in terms of chemical composition and element ratios. None of the litter quality parameters was affected by elevated CO2 for the two Quercus species. Remaining mass in Q. cerris and Q. pubescens litter from elevated CO2 was similar to that from ambient conditions. C. mineralization in Q. pubescens litter from elevated CO2 was lower than that from ambient CO2, but the differnce was insignificant. This effect was not observed for Q. cerris. N. mineralization was higher from litter grown at elevated CO2, but this difference disappeared at the end of the incubation. Litter of Q. pubescens had a higher quality than Q. cerris, and indeed mineralized more rapidly in the laboratory, but not under field conditions

Earthworm Effects on Nitrification Rate and Arginine Amonification in a Calcareous Soil Amended with Urban Sewage Sludge

Introduction: Earthworms are among the most important organisms in soil and their activities can be an indicator of soil quality. These organisms may be influenced by organic wastes application such as sewage sludge and subsequently affect soil quality. One of the quick and easy methods for soil quality monitoring is the use of biological indicators such as microbial activity. It is due to their quick response to changes in the environment. The purpose of this study was to evaluate the effect of earthworms on nitrification rate and arginine ammonification as microbial activity in a calcareous soil amended with urban sewage sludge. Materials and Methods: The studied soil was sampled from Shahrekord University land and sewage sludge belonged to the refinery sludge ponds of shahrekord. Based on dry weight, this organic waste had carbon and nitrogen, approximately 67 and 110 times more than tested soil, respectively. The organic waste in terms of quality and heavy metal concentrations was in class A. Experimental treatments were sewage sludge (without and with 1.5% sewage sludge) and earthworm (no earthworm, Eiseniafoetida from epigeic group, Allolobophracaliginosa from endogeic group and a mixture of the two species) as 2×4 full factorial experiment arranged in a completely randomized design with three replications. After applying sewage sludge, the pots were irrigated three months to achieve a balance in the soil. An adult earthworm per kg of soil was added and in the mixed treatments comparison species were 1:1. To prevent the exit of earthworms, the pots was closed with a thin lace. At the end of the experiment, soil was completely mixed. Part of it was stored in the refrigerator to measure the microbiological parameters. Chemical properties were measured by the air-dried soil. The effectiveness of a factor in the observed changes is shown by partial effect size (Tabachnick and Fidell 2012). So, partial effect size (Eta2p) for each source of variation (SS, earthworm and SS×earthworm) was calculated. Results Discussion: According to Eta2p, the role of sewage sludge application to increase total nitrogen was almost twice the earthworm and had a greater effect on the property. Because of low concentrations of heavy metals and high nutrient in sewage sludge, it increased nitrification rate and arginine ammonification by 16.7 and 62.5 percent, respectively. Considering that the indices represent microbial biomass activity, so we can say sewage sludge application increased theri activities. Sewage sludge application increased total nitrogen, because provided the substrate for heterotrophic bacteria. Consequently, ammonium production improved and stimulated activity of Nitrosomonas and Nitrobacter. There was a positive and significant correlation between total nitrogen, arginine ammonification and nitrification rate, that confirmed the occurrence of this process. Earthworm inoculation affected these two indicators (