Utilization of Kelakai Wetland Plants (Stennochlaena palutris) and Different Doses of Probiotics in Feed Formulations on the Growth of Climbing Perch (Anabas testudineus Bloch)

The aim of the study was to test the provision of feed made from kelakai with the best probiotic dose for the growth of climbing perch. It is expected that the feed formulation made from kelakai in the form of pellets will increase its growth by administering probiotics. The research method used a completely randomized design (CRD) with 4 treatments and 3 replications, rearing for 30 days in an aquarium. Treatment A: feeding pellets with 20% kelakai flour and 0% probiotics, treatment B: feeding pellets with 20% kelakai flour and 5% probiotics treatment C: feeding pellets with 20% kelakai flour and 10% probiotics, treatment D: giving pelleted feed with 20% kelakai flour and 15% probiotics. The results showed that all treatments were not significantly different, the highest relative length growth was in treatment C (20% kelakai flour; 10% probiotics), the highest relative weight growth of climbing perch was in D treatment (20% kelakai; 15% probiotics), survival 100 % in all treatments. Water quality is within a range that supports the growth and survival of climbing perch

Hematological Response Studies of Climbing Perch (Anabas testudineus) Fed with Fermented Feed on the Fermentation of Kelakai Leaves (Stenochlaena palustris)

The purpose of this study was to analyze the nutritional value of fermented feed on kelakai leaves that are feasible as climbing perch feed and the hematological response of climbing perch raised by fermentation-based feeding of kelakai leaves. The study was carried out using an experimental method on the maintenance of climbing perch which was fed based on fermented leaves with 5 treatments and 3 tests. The parameters observed are the nutritional content of feed based on fermented leaves and hematological responses which include erythrocyte, leukocyte, hemoglobin, and hematocrit values. The results of the research on the nutritional content of feed showed that fermented feed on kelakai leaves has a protein content of 27%-31%, crude fiber content of 4%-7%, water content of 12%-14%, ash content of 11%-13%, fat content of 4%-6%. It was concluded that there was a decrease in fiber content and fat content and an increase in protein content, water content, and ash content. It happens gradually in line with the increase in probiotic dose. The results of the study on the hematological response of climbing perch are erythrocytes range from 1.65-3.91, leukocytes range from 223.23-287.43, hematocrit ranges from 15.30-35.07 and hemoglobin ranges from 5.37-10.33. The results of the proximate analysis of feed based on the fermentation of kelakai leaves showed that the values of water content, ash content, crude fiber, protein, and fat were in the recommended range. The hematological response of climbing perch shows good results and is in the normal range

SUBSTITUSI TEPUNG IKAN DENGAN TEPUNG GONDANG (Pila ampulacea) DAN TEPUNG KALAKAI (Stenochlaena palustris (Burm.) bedd) PADA PAKAN IKAN GABUS HARUAN YANG DIPELIHARA DI AKUARIUM

Tujuan dari penelitian ini adalah untuk menganalisis substitusi tepung ikan dengan tepung gondang dan tepung kalakai terhadap pertumbuhan, rasio konversi pakan dan tingkat kelansungan hidup ikan gabus haruan (Channa striata). Penelitian ini menggunakan Rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan.Perlakuan A (pellet tanpa substitusi/Kontrol), B :pellet dengan perbandingan tepung ikan, tepung gondang dan tepung  kalakai (50%: 25%: 25%), C :Pemberian pakan pellet dengan perbandingan tepung ikan, tepung gondang dan tepung  kalakai (25%: 32,5%: 32,5%), D :Pemberian pakan pellet dengan perbandingan tepung ikan, tepung gondang dan tepung  kalakai (0%: 50%: 50%). Parameter yang diamati adalah analisis proksimat pakan, pertumbuhan relatif berat, pertumbuhan relatif panjang, dan kualitas air.Hasil penelitian menunjukkan bahwa hasil uji proksimat terbaik pada komposisi protein perlakuan A (kontrol) 43,5% sedangkan perlakuan dengan subsitusi tepung gondang dan tepung kalakai pada perlakuan B,C dan D berkisar antara 36.22-37,99%. Substitusi tepung gondang dan tepung kalakai mempengaruhi  pertumbuhan relatif panjang, tidak berbeda nyata antar perlakuan, pertumbuhan panjang tertinggi pada perlakuan D, sedangkan  berat relatif  tidak berbeda nyata antar perlakuan, berat relatif tertinggi pada perlakuan D.  Kualitas air: suhu, Oksigen terlarut, pH dan amoniak mendukung kehidupan ikan gabus haruan yang dipelihara

Aeromonas hydrophila Bivalen Vaccines to Improve Body Resistance catfish (Pangasius hypophthalmus) Against Attacks Motile Aeromonas Septicemia

Motile aeromonas septicemia/MAS diseases due to Aeromonas hydrophila infection is an obstacle in cultivation of catfish (Pangasius hypophthalmus). MAS control can be done by administering vaccines. The purpose of this research is to get the right and effective dose of bivalent vaccine for controlling MAS at catfish. Both isolates will be used as vaccines are local strains of A. hydrophila CASO.01.G and SBMI.2. Both of them were isolated from disease catfish in the aquaculture ponds in Cindai Alus and Sungai Batang villages, Banjar Regency, South Kalimantan. Vaccines are made by heating (heat killed) at 100oC for 60 minutes. A total of  13 test catfish measuring 9-13 cm were included in each aquarium. The design used was a completely randomized design with 5 treatments and 3 replications, namely bivalent vaccine with a dose of 109 cells/ml, 108 cells/ml, 107 cells/ml, 106 cells/ml, and control (PBS solution pH 7.0). The highest antibody titers in treatments vaccinated fish at a dose of 107 cells / ml (1,706.67). The effectiveness of the vaccine can be seen by the high RPS value of the treatment dose 109 cells/ml (97.22%); 108 cells/ml (100%); 107 cells/ml (97.22%); and 106 cells/ml (100%). The control MTD value was 1 day with 97.43% mortality, while in the vaccine treatment with dose of 107 cells/ml, there was a MTD repeat value for 1 day, but only 1 fish died. Water quality of this research are supportive and not a cause of fish death during the challenge tes

Chemicals pofile of Kelakai leaves extracts (Stenochlaena palustris) with antioxidant and antibacterial activity against Aeromonas hydrophila

Kelakai (Stenochlaena palustris) is a typical Kalimantan plant that has been used by Banjar and Dayak communities as traditional medicine and vegetable. This study aimed to examine the potential of kelakai leaves extracts as a natural product to inhibit Aeromonas hydrophila growth. This research included kelakai leaves sampling in Banjarbaru, South Kalimantan, Indonesia, extraction (EtOH 1:4 w/v), phytochemical and chemicals profile screening (LCMS), prediction of biological activity (PASS server), antibacterial activity (broth dilution), antioxidant activity (DPPH), total phenol (gallic acid equivalent), total flavonoid (quercetin equivalent), and total alkaloid (caffeine equivalent). The phytochemical screening showed that the kelakai leaves extract contained saponins, tannins, phenolics, flavonoids, alkaloids, anthraquinones, triterpenoids, and steroids. The chemicals profile of the kelakai leaves ethanol extract consisting of alkaloids, alcohols, amines, amine alcohols, amino acids, fatty acids, flavonoids, glycosylglucose, lipid derivatives, monocarboxylic acid, saponins, steroids, and terpenoids. Prediction of biological activity showed kelakai leaves extract an inhibitor of the peptidoglycan glycosyltransferase enzyme and free radical scavenger. The antibacterial assay showed that kelakai leaves extract could inhibit the growth of A. hydrophila. In addition, kelakai leaves extract showed very strong antioxidant potential (IC50 42.47 ± 0.98 µg/mL), with a total phenol content of 193.97 ± 0.11 mg GAE/g, total flavonoid 23.45 ± 0.14 mg QE/g, and total alkaloid 11.74 ± 0.10 mg CE/g. These research findings show that the ethanol extract of kelakai leaves could be antibacterial against A. hydrophila, which is closely related to its antioxidant properties

Penerapan Teknologi Pakan Ikan Mandiri Untuk Kelompok Pembudidaya Ikan “Panle Bersaudara” Desa Danda Jaya, Kabupaten Barito Kuala

Abstrak Permasalahan prioritas yang perlu secepatnya diatasi oleh Kelompok Pembdidaya Ikan ”Panle Bersaudara” adalah masalah semakin mahalnya harga pakan ikan lele dan patin, sehingga diperlukan adanya desiminasi Iptek berupa pembuatan pakan ikan mandiri berbasis bahan baku lokal yang berbiaya murah. Metode penyampaian yang digunakan dalam penerapan Iptek ini adalah: penjelasan teori dan FGD; demonstrasi dan pendampingan. Hasil kegiatan didapat luaran Iptek berupa pakan ikan mandiri yang memenuhi standar gizi untuk ikan patin dan lele yaitu 30%. Telah dilakukan bimbingan teknis pembuatan pakan ikan berupa: memilih bahan baku yang murah tapi bergizi, memformulasi, penimbangan bahan, mencampur dan mengadon, mencetak dan menjemur serta mengeringkan dan menyimpan. Hasil evaluasi terhadap khalayak sasaran dengan uji t, uji dua pihak sebelum dan sesudah penerapan Iptek, ternyata terjadi peningkatan pengetahuan dan keterampilan khalayak sasaran. Faktor pendukung dalam kegiatan ini adalah bahan baku tersedia kontinu, motivasi kuat dalam usaha, sarana prasarana transportasi mendukung, dan tidak terkendala pemasaran ikan. Sedangkan faktor penghambat adalah sulitnya akses modal usahadan anggaran Pemerintah yang terbatas dalam pembinaan. Kata Kunci: Pakan Mandiri, Bahan Baku Lokal, Kelompok Pembudidaya Ikan ”Panle Bersaudara”   Abstract The priority problem that needs to be addressed as soon as possible by the “Panle Bersaudara” Fish Farming Group is the problem of the increasing price of catfish and catfish feed, so that science and technology dissemination is needed in the form of making independent fish feed based on low-cost local raw materials. The delivery methods used in the application of this science and technology are theoretical explanations and FGDs; demonstration and assistance. The results of the activity obtained the output of science and technology in the form of independent fish feed that met the nutritional standards for catfish and catfish, namely 30%. Technical guidance on the manufacture of fish feed has been carried out in the form of choosing cheap but nutritious raw materials, formulating, weighing ingredients, mixing and kneading, printing and drying and drying, and storing. The results of the evaluation of the target audience with the t-test, a two-party test before and after the application of science and technology, show an increase in the knowledge and skills of the target audience. The supporting factors in this activity are the continuous availability of raw materials, strong motivation in business, support transportation infrastructure, and not being constrained by fish marketing. Meanwhile, the inhibiting factors are difficulty accessing business capital and the limited government budget for fostering. Keywords: Independent Feed, Local Raw Materials, “Panle Bersaudara” Fish Cultivator Grou

PEMBERIAN PAKAN KOMERSIL DENGAN PENAMBAHAN PROBIOTIK YANG MENGANDUNG Lactobacillus sp. TERHADAP KECERNAAN DAN PERTUMBUHAN IKAN NILA (Oreochromis niloticus)

The purpose of this research was to determine the effect of probiotics incommercial feed on feed quality, enhance increase bactery number in gut mucose and digestibility of nile tilapia (Oreocromis niloticus) through the provision of environmental friendly probiotic feed, determine the effective doses of probiotics in feed that can support the growth fish. The research was conducted for 6 (six)months, from September 2012 until February 2013. The design used in this study was completely randomized design with 4 (four) treatments and 3 (three) replicates, were treatment A (control), B (Probiotics 3%), C (Probiotics 5%) and D (Probiotic7%).The results showed that the addition of probiotic containing Lactobacillus sp.feeding with doses of 3%, 5% and 7% were able to improve the nutritional content,protein values between 32,48%-34,19% and lower crude fiber with values between 5,04-5,70% compared with no probiotics. The addition of probiotics in feed with a percentage of 3%, 5%, and 7% can increase digestibility of nile tilapia and effectiveto increase the nile weight and length growth of nile tilapia

The Compliance of Ship's Crew Toward International Convention Implementation for Controlling of Ballast Water and Sediment from Ships In Offshore Port of Taboneo

This study aims to analyze the level of compliance of the ship’s crew toward The BWM Convention. (Ballast Water Management Convetion). This research is kind of qualitative using a research design that relates the independent variables and the dependent variable at the same time. The study was conducted in the Offshore Port of Taboneo with a total sample of 54 respondents. The research data is primary data from interview by using questioner and secondary data obtained from interview and literature study. The results showed that the Compliance of the crew on the ship that has been equipped with Ballast Water Treatment System (BWTS) is 66.67 % of respondents fall into the category approach to obedient and 33.33 % of respondents approach to non-obedient category. So also the compliance of crew on board ship that has not been equipped with BWTS there are as many as 60.61 % of respondents fall into the category approach to obedient and 39.39 % of respondents approach to non-obedient category