3 research outputs found
Comparing the expression levels of mRNA for MMP-7 in gastric mucosa of patients with H. pylori infection and uninfected patients
Background and purpose: The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines are altered in Helicobacter pylori (H. pylori) infected gastric mucosa. Matrix metalloproteinases (MMP) are a family of zinc-dependent homologous enzymes digesting most of the components of the extracellular matrix and basement membrane and are involved in remodeling and functioning of the biological processes. The purpose of this study was to compare gene expression of matrix metalloproteinase-7 (MMP-7) in patients with H. pylori-infected and uninfected individuals with gastrointestinal diseases. Materials and methods: This study was conducted in 50 H. pylori-negative patients and 50 H. pylori-positive patients being admitted to Shahrekord Hajar Hospital due to gastrointestinal diseases in 2014. The participants’ demographic information was collected and sampling was done. First DNA was extracted, and then PCR was performed to check for the presence of 16sRNA and UreC. The RNA from each sample was also extracted and cDNA was prepared. Afterwards, the expression of MMP-7 was measured by real time-PCR using specific primers and probes. Results: MMP-7 mRNA expression was significantly higher in biopsies of H. pylori-infected patients compared to that in H. pylori-uninfected patients (P<0.0001). Conclusion: Increased expression of MMP-7 can be effective in inflammatory response and development of the disease. It could be used as a key marker for early diagnosis of gastrointestinal diseases and gastric cancer. © 2016, Mazandaran University of Medical Sciences. Engineering. All rights reserved
PREVALENCE AND ASSOCIATION OF HELICOBACTER PYLORI INFECTION WITH GASTROINTESTINAL DISEASE
Prevalence of cagA and babA2 genes in Helicobacter Pylori strains Isolated from Iranian gastrointestinal disorder patients and their gas-tritis classification
Helicobacter pylori is a spiral gram negative flagellate bacteria and localize in the stomach. H.p infection is a worldwide
health problem and identified as an important cause of gastritis and gastric cancer and its ability to develop such disorders is
related to its virulence factors and environment. cagA is the most important Hp virulence factor that directly penetrate into
gastric epithelial cells by bacterial secretion system (T4SS) from pathogenicity island (PAI) and disrupts cell homeostasis.
Adherence factors are significant for bacterial colonization and suitable function of other virulence factor. Blood group
antigen binding adhesion (babA) is an outer membrane protein (OMP) that binds to ABO blood group antigen and can
stimulate inflammatory response in gastric cells. Our main target was to determine the roles and prevalence of cagA and
babA2 virulence factor in gastrointestinal disorders in Iranian patients. Existences of These factors were determined by PCR
in 218 patients with gastrointestinal disorders. Semi-quantitative methods of scoring according to the Updated Sydney
classification system were used for detection of H.pylori density, neutrophil and monocyte cells infiltration. A high prevalence
of cagA positive (81.4%) and babA2 positive (35%) were found. The most combined genotype (cagA&babA2) prevalence
was found in gastritis & ulcer (100%) (P < 0.001). High prevalence of cagA positive observed in active inflammation phase
76.9% and high prevalence of babA2 positive was in active phase 61.1% of H.pylori gastritis (P=0.001) . Results of this
study showed information about the high prevalence of cagA genes in H.pylori infected patients and their rolls in active
gastrointestinal disorder