Skewed distribution of proinflammatory CD4+CD28null T cells in rheumatoid arthritis

Expanded populations of CD4+ T cells lacking the co-stimulatory molecule CD28 (CD4+CD28null T cells) have been reported in several inflammatory disorders. In rheumatoid arthritis, increased frequencies of CD4+CD28null T cells in peripheral blood have previously been associated with extra-articular manifestations and human cytomegalovirus (HCMV) infection, but their presence in and contribution to joint manifestations is not clear. In the present article we investigated the distribution of CD4+CD28null T cells in the synovial membrane, synovial fluid and peripheral blood of RA patients, and analysed the association with erosive disease and anti-citrullinated protein antibodies. CD4+CD28null T cells were infrequent in the synovial membrane and synovial fluid, despite significant frequencies in the circulation. Strikingly, the dominant TCR-Vβ subsets of CD4+CD28null T cells in peripheral blood were often absent in synovial fluid. CD4+CD28null T cells in blood and synovial fluid showed specificity for HCMV antigens, and their presence was clearly associated with HCMV seropositivity but not with anti-citrullinated protein antibodies in the serum or synovial fluid, nor with erosive disease. Together these data imply a primary role for CD4+CD28null T cells in manifestations elsewhere than in the joints of patients with HCMV-seropositive rheumatoid arthritis

CD4CD28T cells from peripheral blood and synovial fluid show human cytomegalovirus specificity

<p><b>Copyright information:</b></p><p>Taken from "Skewed distribution of proinflammatory CD4CD28T cells in rheumatoid arthritis"</p><p>http://arthritis-research.com/content/9/5/R87</p><p>Arthritis Research & Therapy 2007;9(5):R87-R87.</p><p>Published online 7 Sep 2007</p><p>PMCID:PMC2212553.</p><p></p> The functional capacity of CD4CD28T cells in peripheral blood (PB) and synovial fluid (SF) was investigated after stimulation of mononuclear cells from paired PB and SF from rheumatoid arthritis patients by plate-bound anti-CD3 antibodies. The reactivity of CD4CD28T cells in paired PB and SF to human cytomegalovirus (HCMV) antigens was analysed after stimulation by the pp65 or immediate early (IE) antigens

Skewed distribution of CD4CD28T cells in synovial fluid and peripheral blood

<p><b>Copyright information:</b></p><p>Taken from "Skewed distribution of proinflammatory CD4CD28T cells in rheumatoid arthritis"</p><p>http://arthritis-research.com/content/9/5/R87</p><p>Arthritis Research & Therapy 2007;9(5):R87-R87.</p><p>Published online 7 Sep 2007</p><p>PMCID:PMC2212553.</p><p></p> Paired samples of synovial fluid (SF) and peripheral blood (PB) from 128 rheumatoid arthritis patients were compared for the frequency of CD4CD28T cells by flow cytometry. Frequencies of CD4CD28T cells in SF tend to be higher in patients with large populations in PB. Comparison of the frequency of CD4CD28T cells in SF from two different synovial compartments. Open circle, elbow; open squares, shoulder joints. Frequencies of CD4CD28T cells in SF and PB in patients seronegative and seropositive for human cytomegalovirus (HCMV). Frequencies of CD4CD28T cells in paired PB and SF of patients seropositive for HCMV