Flow chart of study subjects.

<p>Flow chart of study subjects.</p

Multivariate analysis in prediction of miscarriages (n = 3058).

<p>Multivariate analysis in prediction of miscarriages (n = 3058).</p

Comparisons between miscarriage and continued pregnancy groups (n = 3058).

<p>Comparisons between miscarriage and continued pregnancy groups (n = 3058).</p

Characteristics of study participants (n = 3058).

<p>Characteristics of study participants (n = 3058).</p

Additional file 1: of Fetal growth restriction in rural Bangladesh: a prospective study

Table S1. Distribution of gestational age (GA, week) at the 14, 19 and 30 weeks clinic visits, with LMP dates. Table S2. Growth chart for fetal biparietal diameter (BPD, mm) by percentile with SD. Table S3. Growth chart for fetal head circumference (HC, mm) by percentile with SD. Table S4. Growth chart for fetal abdominal circumference (AC, mm) by percentile with SD. Table S5. Growth chart for fetal femur length (FL, mm) by percentile with SD. Table S6. Growth chart for estimated fetal weight (g) by percentile with SD. (DOC 348 kb

Transcriptome Profile of the Chicken Thrombocyte: New Implications as an Advanced Immune Effector Cell

<div><p>Thrombocytes are nucleated platelets involved in immune functions such as pathogen recognition and release of pro-inflammatory bioactive compounds when exposed to bacterial and viral molecules. However, the complete role of these cells in innate and adaptive immune responses is not understood, and little is known about their biology at the molecular-genetic level. Highly sensitive RNA-sequencing technologies were used to analyze the complete transcriptome of thrombocytes for the first time with analytical resolution focused on cell-based components of the immune system/response. Amongst all the genes listed in the current chicken genome assembly, 10,041 gene transcripts were found in the chicken thrombocyte. After 1-hour <i>in vitro</i> stimulation with lipopolysaccharide (LPS, <i>Salmonella minnesota</i>), 490 genes were upregulated and 359 genes were downregulated, respectively, with at least a 1-fold change relative to unexposed thrombocytes. Additionally, by constructing a <i>de novo</i> assembly, we were able to identify a total of 3,030 novel genes in the thrombocyte transcriptome. The information generated here is useful in development of novel solutions to lower the economic burden and zoonotic threat that accompanies infectious diseases for birds and fish. In addition, the resources created here have translational utility as a model system to find orthologous genes and genes related to its enucleated counterpart, the platelet.</p></div

Additional file 1: Table S1. of Temperature during pregnancy influences the fetal growth and birth size

Regression coefficients for the association between birth length and temperature at different gestational points (using 1-week moving average temperature; n = 3267). (DOC 34 kb

A volcano plot of genes with differential expression profiles after a 1 hr treatment with LPS.

<p>Positive values on the X-axis indicate genes with increased transcript abundance, and negative values indicate genes with decreased transcript abundance. Black dots indicate genes that are greater than the false discovery rate (fdr) of 0.05, and the vertical blue bars delineate a threshold of 1 fold change.</p

Top 10 up- and down-regulated genes in response to <i>in vitro</i> stimulation of chicken thrombocyte.

<p>Top 10 up- and down-regulated genes in response to <i>in vitro</i> stimulation of chicken thrombocyte.</p

Panther Over Representation Test.

<p>Panther Over Representation Test.</p