2 research outputs found
Microsatellites within the feline androgen receptor are suitable for X chromosome-linked clonality testing in archival material
Objectives A hallmark of neoplasms is their origin from a single cell; that
is, clonality. Many techniques have been developed in human medicine to
utilise this feature of tumours for diagnostic purposes. One approach is X
chromosome-linked clonality testing using polymorphisms of genes encoded by
genes on the X chromosome. The aim of this study was to determine if the
feline androgen receptor gene was suitable for X chromosome-linked clonality
testing. Methods The feline androgen receptor gene, was characterised and used
to test clonality of feline lymphomas by PCR and polyacrylamide gel
electrophoresis, using archival formalin-fixed, paraffin-embedded material.
Results Clonality of the feline lymphomas under study was confirmed and the
gene locus was shown to represent a suitable target in clonality testing.
Conclusions and relevance Because there are some pitfalls using X chromosome-
linked clonality testing, further studies are necessary to establish this
technique in the cat