Diversity, phylogeny and intraspecific variability of Paradiplozoon species (Monogenea: Diplozoidae) parasitizing endemic cyprinoids in the Middle East

Diplozoidae are common monogenean ectoparasites of cyprinoid fish, with the genus Paradiplozoon being the most diversified. Despite recent studies on Diplozoidae from Europe, Africa and Asia, the diversity, distribution and phylogeny of this parasite group appears to be still underestimated in the Middle East. The objective of this study was to investigate the diversity, endemism and host specificity of diplozoids parasitizing cyprinoid fish from the Middle East, considering this region as an important historical interchange of fish fauna, and to elucidate the phylogenetic position of Middle Eastern Paradiplozoon species within Diplozoidae. Four Paradiplozoon species were collected from 48 out of 94 investigated cyprinoid species. Three known species, Paradiplozoon homoion, Paradiplozoon bliccae and Paradiplozoon bingolensis, were recorded on new cyprinoid host species, and a new species, Paradiplozoon koubkovae n. sp., was recorded on Luciobarbus capito and Capoeta capoeta from the Caspian Sea basin in Iran and Turkey. Paradiplozoon bliccae, exhibiting a wide host range in the Middle East, expressed both morphological and genetic intraspecific variabilities. The four Paradiplozoon species collected in the Middle East were placed in divergent clades, showing the rich evolutionary history of diplozoid parasites in the Middle East. Our study also revealed that two lineages of African diplozoids have a Middle Eastern origin. We stress the importance of applying an integrative approach combining morphological, ecological and molecular methods to reveal the real diversity of diplozoids

Numerical study of mixing and heat transfer in mixed electroosmotic/pressure driven flow through T-shaped microchannels

This paper investigates mixing and heat transfer characteristics of mixed electroosmotic–pressure driven flow within a T-shaped microchannel. Two different mechanisms were proposed to enhance mixing efficiency within the channel. First, a non-uniform DC electric potential was embedded on the horizontal section of the channel which causes the straight streamlines to deviate and thus increases interfacial contact area and mass diffusion by absorbing ions into electrodes. Next, a ribbed channel configuration was proposed. It is shown that due to separation, recirculation zones appear behind the obstacles which helps in enhancing mixing efficiency. Finally a combined effect of ribbed channel and wall zeta potential was investigated. The numerical results show that due to presence of non-uniform zeta potential, the induced vortices are pushed into the middle of the channel and thus mixing enhances drastically by using this scheme. Intensity of mixing was computed for all cases and it was found that for any Reynolds number, there exists a Schmidt number value which below that specific value, imposing electric field reduces mixing. Finally, heat transfer was studied for all introduced cases for a constant uniform heat flux imposed at the walls. The obtained local average Nusselt number indicates that employing zeta potential has an important effect on the mixture temperature within the channel and at the outlet of the channel

Unsteady pulsating characteristics of the fluid flow through a sudden expansion microvalve

This paper investigates the unsteady characteristics of flow in a specific type of microvalve with sudden expansion shape. The resultant vortex structures cause different flow resistance in forward and backward flow directions. This may be used in applications such as a microvalve in micropump system and MEMS-based devices. A time-varying sinusoidal pressure was set at the inlet of the microchannel to produce unsteadiness and simulate the pumping action. The existence of block obstacle and expansion shoulders leads to various sizes of vortex structures in each flow direction. All simulation results are based on the numerical simulation of two-dimensional, unsteady, incompressible and laminar Navier–Stokes equations. Two fundamental parameters were varied to investigate the vortex growth throughout the time: the frequency of the inlet actuating mechanism (1 Hz ≤ f ≤ 1,000 Hz) and the amplitude of the inlet pressure. In this way, one can see the effect of actuation mechanism on the onset of separation and follow the size and duration of the vortex growth. In order to better understand the effect of geometry and frequency on flow field, the pressure and velocity distributions are studied through one cycle. Strouhal number is calculated for frequency, and a critical value of f = 250 Hz is found for St = 1. The obtained results provide a deep insight into the physics of unsteady flow in valveless micropumps and leads to better use of current design as a part of microfluidic system

A Proposed Approach towards Quantifying the Resilience of Water Systems to the Potential Climate Change in the Lali Region, Southwest Iran

Computing the resilience of water resources, especially groundwater, has hitherto presented difficulties. This study highlights the calculation of the resilience of water resources in the small-scale Lali region, southwest Iran, to potential climate change in the base (1961–1990) and future (2021–2050) time periods under two Representative Concentration Pathways, i.e., RCP4.5 and RCP8.5. The Lali region is eminently suitable for comparing the resilience of alluvial groundwater (Pali aquifer), karst groundwater (Bibitarkhoun spring and the observation wells W1, W2 and W3) and surface water (Taraz-Harkesh stream). The log-normal distribution of the mean annual groundwater level and discharge rate of the water resources was initially calculated. Subsequently, different conditions from extremely dry to extremely wet were assigned to the different years for every water system. Finally, the resilience values of the water systems were quantified as a number between zero and one, such that they can be explicitly compared. The Pali alluvial aquifer demonstrated the maximum resilience, i.e., 1, to the future climate change. The Taraz-Harkesh stream, which is fed by the alluvial aquifer and the Bibitarkhoun karst spring, which is the largest spring of the Lali region, depicted average resilience of 0.79 and 0.59, respectively. Regarding the karstic observation wells, W1 being located in the recharge zone had the lowest resilience (i.e., 0.52), W3 being located in the discharge zone had the most resilience (i.e., 1) and W2 being located between W1 and W3 had an intermediate resilience (i.e., 0.60) to future climate change

A Proposed Approach towards Quantifying the Resilience of Water Systems to the Potential Climate Change in the Lali Region, Southwest Iran

Computing the resilience of water resources, especially groundwater, has hitherto presented difficulties. This study highlights the calculation of the resilience of water resources in the small-scale Lali region, southwest Iran, to potential climate change in the base (1961–1990) and future (2021–2050) time periods under two Representative Concentration Pathways, i.e., RCP4.5 and RCP8.5. The Lali region is eminently suitable for comparing the resilience of alluvial groundwater (Pali aquifer), karst groundwater (Bibitarkhoun spring and the observation wells W1, W2 and W3) and surface water (Taraz-Harkesh stream). The log-normal distribution of the mean annual groundwater level and discharge rate of the water resources was initially calculated. Subsequently, different conditions from extremely dry to extremely wet were assigned to the different years for every water system. Finally, the resilience values of the water systems were quantified as a number between zero and one, such that they can be explicitly compared. The Pali alluvial aquifer demonstrated the maximum resilience, i.e., 1, to the future climate change. The Taraz-Harkesh stream, which is fed by the alluvial aquifer and the Bibitarkhoun karst spring, which is the largest spring of the Lali region, depicted average resilience of 0.79 and 0.59, respectively. Regarding the karstic observation wells, W1 being located in the recharge zone had the lowest resilience (i.e., 0.52), W3 being located in the discharge zone had the most resilience (i.e., 1) and W2 being located between W1 and W3 had an intermediate resilience (i.e., 0.60) to future climate change

New Oligoneuriidae (Insecta, Ephemeroptera) from Iran

Two new species of the mayfly family Oligoneuriidae are described based on larval specimens recently collected in Iran. The first new species, Oligoneuriella tuberculata Godunko & Staniczek, sp. nov., can be distinguished from all its congeners by the presence of pronounced protuberances posteromedially on abdominal terga, highly reduced paracercus, large lamella of gill I, and setation on hind margin of middle and hind femora confined to their basal halves. The second species, Oligoneuriopsis villosus Bojková, Godunko, & Staniczek, sp. nov., remarkably belongs to a mostly Afrotropical genus. The new species clearly differs from all its congeners in the shape of setae on the surface of gills and terga, pattern of body colouration, and the shape of posterolateral projections of abdominal segments. Except for the species description, the generic diagnosis of Oligoneuriopsis Crass, 1947 is briefly discussed. COI barcode sequences of both new species are provided and molecular species delimitation is tested using distance-based and likelihood-based approaches, with both new species unambiguously recognised as separate lineages. The analysis of COI also corroborates the respective affinities of both new species, estimated based on morphology. The two new species of Oligoneuriidae described herein highlight the importance of the Middle East as a centre of diversity of this mayfly family within the Palaearctic