Evaluación del comportamiento epidemiologico del sharka en San Rafael, Mendoza

PosterEl Plum pox virus ( es el agente causal del Sharka la enfermedad viral más importante de los frutales de carozo, principalmente de ciruelos, damascos y durazneros En Argentina fue detectada en el 2004 en San Juan y 2006 en San Rafael, Mendoza Luego de erradicar los focos iniciales, declarar las zonas en cuarentena y un estricto control de las plantas madres de vivero, se ha podido prevenir la dispersión de la enfermedad Sin embargo, aún falta información de lo que sucede en los montes comerciales cercanos a los focos iniciales de infección daños producidos y el comportamiento del virus bajo nuestras condiciones agroecológicasInstituto de Patología VegetalFil: Piglionico, Damián Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Lucero, Vanesa Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentin

First report of a ‘Candidatus phytoplasma pyri’ strain in Argentina

During the 2015 growing season, peach plants (Prunus persica) showing chlorotic, curling leaves and midrib thickening were detected in production fields in Mendoza province (Midwest region, Argentina). These symptoms resemble those described for Peach Yellow Leaf Roll disease, a ‘Candidatus Phytoplasma pyri’ related strain. By PCR of the 16S rDNA gene, a phytoplasma was detected in symptomatic samples. The RFLP profile, sequence identity and phylogenetic analyses showed that the Peach Yellows phytoplasma belongs to group 16SrX-C and is identical to those described as ‘Candidatus Phytoplasma pyri’. This is the first report of a phytoplasma from 16Sr group X infecting peach trees in Argentina and in South America.Instituto de Patología VegetalFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentin

Spiraea sp. new natural host of Plum pox virus (Sharka)

Plum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings, were observed on leaves of an ornamental plant of the Rosaceae family “Bridal Crown” (Spiraea sp.) in the garden of a house within the PPV quarantine area in San Rafael, Mendoza province. Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. A 243 bp PCR fragment amplified from the Spiraea samples, and corresponding to the carboxy-terminal region of PPV coat protein gene was isolated, sequenced, and deposited in GenBank (MN587037). It showed 99% identity to other PPV isolates. RT-qPCR, confirmed the virus as being an isolate of the PPV D strain previously found in the quarantine areas. Our results confirm PPV natural infection on a new ornamental host, “Bridal Crown”, which does not belong to the genus Prunus, warning of the potential danger of the species as a host of, and a source of, inoculum for PPV.EEA JunínFil: Piglionico, Damián Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Lucero, Vanesa Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentin

Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina

II International Symposium on Plum Pox VirusSharka, caused by Plum pox virus (PPV), is the most important viral disease of stone fruits worldwide. PPV was detected for first time in Argentina in 2004 and since then many efforts have been made to eradicate it. To optimize sampling and virus detection by DAS-ELISA, 8 PPV-positive prune trees (P. domestica, cv. 'D´agen') were selected and sampled monthly from September 2008 to August 2010. The plant tissue collected was the one available at the sampling time (leaves, flowers or buds). The samples were taken from each of the four tree scaffolds at three different heights (basal, medium and apical). Variability of absorbance levels in each month was analyzed with Principal Components Analysis and sampling schemes were compared with non parametric variance analysis. This is the first study made in Argentina to tracking PPV relative virus concentration throughout the year. As described previously by many authors in the north hemisphere the highest levels of virus concentration, in both studied years, were found in spring (October and November) using flowers and young leaves. The scaffold basal height showed the highest PPV concentration in these two months. High variation of relative virus concentration levels were found among months, within and between years. Regarding different parts of the plant, relative virus concentration decreased from the base to the apical part of the scaffold, indicating that sampling should be performed at a basal height. This study will contribute to the optimization of DAS-ELISA PPV detection in Argentina.EEA JunínFil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Fuentes, C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Teich, Ingrid. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin