1 research outputs found
Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with Roles in Plant Cell Wall Degradation and Virulence
Rhizoctonia solani AG 4 is a soilborne
necrotrophic fungal plant pathogen that causes economically important
diseases on agronomic crops worldwide. This study used a proteomics
approach to characterize both intracellular proteins and the secretome
of <i>R. solani</i> AG 4 isolate Rs23A under several
growth conditions, the secretome being highly important in pathogenesis.
From over 500 total secretome and soluble intracellular protein spots
from 2-D gels, 457 protein spots were analyzed and 318 proteins positively
matched with fungal proteins of known function by comparison with
available <i>R. solani</i> genome databases specific
for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized
to possible cellular locations and functional groups and for some
proteins their putative roles in plant cell wall degradation and virulence.
The majority of the secreted proteins were grouped to extracellular
regions and contain hydrolase activity