Differentiation status of cells with abnormal sex-karyotypes.

<p><b>(</b>A) FABP2/IFABP expression in a control and (B) transplant patient sample with an example of an XXY cell. Brackets indicate differentiated (high Fabp2/Ifabp expression, black brackets) and undifferentiated (low Fabp2/Ifabp expression, gray brackets) regions of epithelium within each sample. (C) Enlarged view of boxed region from <i>B</i>, in an adjacent tissue section stained for Lamin B1.(D) Enlarged view of boxed region from panel C, showing X- (green) and Y- (red) chromosomes and Lamin B1 (white). Arrowhead indicates the same nucleus in panels C and D. Dashed lines indicate boundaries of epithelial and non-epithelial compartments.</p

Frequency of nuclei with 2 or 3 X-chromosomes.

<p>Percent of Y-chromosome-positive nuclei with two or three X-chromosomes, observed in epithelial (epi) and non-epithelial (non-epi) compartments of normal male and male-into-female gender mismatched bone marrow transplant patients (transplant). *, p = 0.0016, Fisher's exact test.</p

Epithelial compartmentalization and sex-karyotyping of intestinal cells.

<p>(A) Hematoxylin and Eosin stained intestinal biopsy; epithelial compartment is labeled. (B) Adjacent tissue section to that from panel A stained for X- (green) and Y- (red) chromosomes and Lamin B1 (white). Arrows indicate Y-chromosome-positive epithelial cells and the arrowhead points to a Y-chromosome-positive non-epithelial cell. Inset shows a sub-region stained for cytokeratin (blue); arrows and arrowhead serve as positional references. (C) Enlarged views of cells indicated in panel B by arrows and arrowhead; sex-karyotype is indicated for each. (D) Independent patient sample also stained for X- (green) and Y- (red) chromosomes and Lamin B1 (white). Arrows indicate Y-chromosome-positive epithelial cells. Dashed lines in all panels indicate boundaries of epithelial and non-epithelial compartments.</p