Possible schematic presentation for the formation of ZnO-NPs.

<p>Possible schematic presentation for the formation of ZnO-NPs.</p

Reactive Oxygen Species Mediated Bacterial Biofilm Inhibition via Zinc Oxide Nanoparticles and Their Statistical Determination - Figure 1

<p>(a) Shows the low magnification TEM image of the ZnO-NPs, (b) HR-TEM image shows the difference between two lattice fringes, which is about 0.265 nm and consistent with The HR-TEM observations, which indicate the crystallinity of the synthesized products. (c) Typical XRD pattern of grown ZnO-NPs. whereas (d) shows the AFM image of ZnO-NPs.</p

Reactive Oxygen Species Mediated Bacterial Biofilm Inhibition via Zinc Oxide Nanoparticles and Their Statistical Determination - Figure 2

<p>(a) Inhibition on <i>P.aeruginosa</i> biofilm formation at different concentration of ZnO-NPs. (b) ZnO-NPs induced oxidant generation in bacterial cells treated with various concentrations of ZnO-NPs (0, 5, 10, 25, 50 and 100 µg/mL) incubated for 24 h.</p

Zone of inhibition of Bacterial growth of <i>P.aeruginosa</i> with increasing concentration of ZnO-NPs done in triplicate manner.

<p>Zone of inhibition of Bacterial growth of <i>P.aeruginosa</i> with increasing concentration of ZnO-NPs done in triplicate manner.</p

Test of precision of the proposed method for ZnO-NPs.

<p>Test of precision of the proposed method for ZnO-NPs.</p

Bio-TEM images of: (a) <i>P.aeruginosa</i> (b) <i>P. aeruginosa</i> with ZnO-NPs.

<p>(c-f) Possible proposed pictorial mechanism.</p

Reactive Oxygen Species Mediated Bacterial Biofilm Inhibition via Zinc Oxide Nanoparticles and Their Statistical Determination - Figure 4

<p>(a) The absorption spectra of nanoparticles of ZnO, control and nanoparticles of ZnO with control (<i>P.aeruginosa</i>). (b) Optimizion of pH range (2 to 8.0).</p