Antidiabetic and Antioxidant Effect of Scoparia dulcis in Alloxan induced Albino Mice

Abstract: Herbal drugs are frequently considered to be less toxic and also free from side effects, than synthetic ones. Hence, the present study was designed to evaluate antidiabetic and antioxidant principle of the ethanolic extract of Scoparia dulcis (EESD) for its antidiabetic and antioxidant potential against alloxan-induced diabetic mice. Hypoglycemic effect was evaluated in normal and alloxan induced diabetic mice The oral administration of plant extract at a dose of 100 & 200 mg/kg body weight were given to fasting glucose loaded (200 mg/kg body weight) mice with regard to normal control during 3 hr. study period and in alloxan-induced (150 mg/kg body weight) diabetic mice in comparison with reference drug, Metformin (600µg/kg) during 2 weeks study period. Considerable fall in elevated blood glucose level was observed in the normoglycemic (p<0.05) and alloxan induced diabetic (p<0.001) mice. The extract, at a dose of 100 & 200 mg/kg body weight showed glucose level reduction of 31.87% & 46.97% respectively in alloxaninduced diabetic mice while 50.74% was found for Metformin after 2 weeks. The antioxidant potential of ethanolic extract of plant was assessed by DPPH (1, 1-Diphenyl, 2-picryl-hydrazyl) free radical scavenging assay at 517 nm and total antioxidant capacity by phosphomolybdenum method at 695 nm.The extract showed petite free radical scavenging capacity (IC 50 value 243.82 μg/ml for plant extract and 58.92 μg/ml for Ascorbic acid) and moderate total antioxidant capacity compared with Ascorbic acid. These results demonstrate the antidiabetic and antioxidant potential of ethanolic extracts of Scoparia dulcis and suggests that the plant may have therapeutic value in diabetes and related complications

Assessment of Plant Growth Promoting and Abiotic Stress Tolerance Properties of Wheat Endophytic Fungi

The aims of the present work were to isolate and characterize fungal endophytic communities associated with healthy wheat (Triticum aestivum L.) plants, collected from the North China. Segregated endophytes were screened for their PGP traits, abiotic stresses (heavy metals, salinity, drought, and temperature), and antibiotic sensitivity. A total of 16 endophytic fungi were isolated using the culture-dependent approach from different tissue parts of wheat plants. Based upon their internal transcribed spacer (ITS) rDNA gene sequencing, 15 out of 16 isolates were selected for further analysis. In the contemporary investigation, a number of the tested endophytes exhibited fairly good 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) (0.03±0.011 to 1.43±0.01 µmol α-KB mg−1 protein hr−1), indole acetic acid (IAA) (1.125±0.04 to36.12±0.004µgml−1), and phosphate solubilizing index (PSI) (2.08±0.03to5.16±0.36) activities. More than 30% isolates gave positive result for siderophore and ammonia tests, whereas all exhibited catalase activity but only 2 (582PDA1 and 582PDA11) produced hydrogen cyanide. Trichoderma strains showed salt, heavy metals, and drought tolerance at high levels and also exhibited resistance to all the tested antibiotics. Strain 582PDA4 was found to be the most temperature (55°C) tolerant isolate. The findings of this study indicated that the microbial endophytes isolated from wheat plants possessing a crucial function to improve plant growth could be utilized as biofertilizers or bioagents to establish a sustainable crop production system

Investigation on Antioxidant Activities of Six Indigenous Plants of Bangladesh

Abstract: In the present study, the antioxidant potential of methanolic extracts of the leaves of Aegle marmelos Corr.(Local Name-Bel), Abroma augusta Linn (Local Name-Ulot Kambal), Lagerstroemia speciosa (Local Name-Jarul), Cassia fistula (Local Name-Bador Lathi), Anthocephalus chinensis (Local Name-Kadam), Syzygium cumini Skeel (Local Name-Jam), was evaluated. The extracts were investigated for its antioxidant activity using hydrogen donation assay method. The extracts showed significant antioxidant activities in hydrogen donation assay compared to the standard antioxidant (Ascorbic Acid) in a dose dependent manner. All the extracts showed potent antioxidant activity, of which the methanolic 50 extract of Abroma augusta Linn. demonstrated the strongest antioxidant activity with the IC value of 51.9786mg ml . The other extracts of plants also showed significant antioxidant activity. -

Antidiabetic and antidiarrheal effects of the methanolic extract of Phyllanthus reticulatus leaves in mice

Objective: To assess the anti-diabetic and antidiarrheal activity of methanolic extract of Phyllanthus reticulates (P. reticulates) leaves in an animal model. Methods: Phytochemical screening of methanolic extract of P. reticulatus leaves has been performed. Antidiabetic activity have been done by OGTT, normoglycemic hyperglycemia and alloxan induced diabetic mice. Plant extracts (150 mg/kg and 300 mg/kg, b.w.) were administered orally in fasting glucose loaded mice with regard to normal control and in alloxan induced (110 mg/kg body weight i.p.) diabetic mice in comparison with reference drug Metformin hydrochloride (100 mg/kg) during 7 day test period. Antidiarrheal test was conducted by castor oil and magnesium sulfate. Results: Findings confirmed that the continuous post-treatment for 7 days with both extracts showed significant (P < 0.05) hypoglycemic activity in OGTT, normoglycemic and alloxan induced mouse models. Castor oil and Magnesium sulfate induced diarrheal test of the extract (200 and 400 mg/kg) has given significant effect in compairing to control diarrheal group. Conclusion: Methanolic extract of P. reticulatus leaves have shown significant antidiabetic and antidiarrheal properties

Antidiabetic and antioxidant effect of Scoparia dulcis in alloxan induced albino mice

Herbal drugs are frequently considered to be less toxic and also free from side effects, than synthetic ones. Hence, the present study was designed to evaluate antidiabetic and antioxidant principle of the ethanolic extract of Scoparia dulcis (EESD) for its antidiabetic and antioxidant potential against alloxan-induced diabetic mice. Hypoglycemic effect was evaluated in normal and alloxan induced diabetic mice The oral administration of plant extract at a dose of 100 & 200 mg/kg body weight were given to fasting glucose loaded (200 mg/kg body weight) mice with regard to normal control during 3 hr. study period and in alloxan-induced (150 mg/kg body weight) diabetic mice in comparison with reference drug, Metformin (600μg/kg) during 2 weeks study period. Considerable fall in elevated blood glucose level was observed in the normoglycemic (