Ecological Distribution of Virulent Multidrug-Resistant Staphylococcus aureus in Livestock, Environment, and Dairy Products

Staphylococcus aureus is one of the most common causes of mastitis, leading to severe economic losses in the dairy industry. It is also zoonotic, with potential risks to public health. This study aimed to detect the occurrence of S. aureus-resistant strains isolated from cattle, buffalo, their environment, milk and dairy products; and to investigate the extent of animal, ecological, and food contamination by methicillin-resistant (MRSA) or enterotoxigenic S. aureus. Samples (n = 350) were collected from four animal (two cattle and two buffalo) farms, i.e., their environment. Thirty Karish cheese samples were collected from 10 markets in Mansoura, Egypt. S. aureus was detected in 17.9%, 17.6%, and 16.7% of samples collected from cattle, buffalo and Karish cheese, respectively. About 19% of isolated S. aureus strains carried the mecA gene. The distribution of the mecA gene was high in isolates from Karish cheese (60%), followed by samples collected from buffalo (16.2%) and cattle (16%). More than 34% of isolated S. aureus strains were enterotoxigenic, and the presence of enterotoxin genes was higher in isolates from Karish cheese (80%) than those from cattle (48%) and buffalo (18.9%). The most predominant enterotoxin gene among isolated S. aureus strains was the sea gene (26.9%), followed by sec (4.5%) and sed (3%) genes. Isolated strains were resistant to clindamycin (100%), kanamycin (97%), nalidixic acid (86.6%), cefotaxime (73.1%) sulphamethazole—trimethoprim (65.6%). Meanwhile, 95.5%, 94%, 86.6% and 77.7% of S. aureus strains were sensitive to ciprofloxacin, amikacin, imipenem and both cefoxitin and gentamycin, respectively. In conclusion, the presence of enterotoxigenic- and methicillin-resistant S. aureus strains in animals, their environment, and dairy products represents a public health concern, particularly in small-scale dairy farms in Egypt. To reduce the risk of infection of livestock and humans with resistant strains, strict regulations and guidelines for antimicrobial use in such a system are urgently required

Evaluating Heat Island Effect at University Campus with Reference to LEED v4.

The growing complexity in environmental degradation requires a serious action for adopting sustainable initiatives in the different institutions design and operation. Leadership in Energy and Environmental Design (LEED), is the most widely used green building rating system in the world that helps institutions to evaluate their current sustainable performance, and provides a concise guidance for identifying and implementing practical and measurable solutions. One of the important challenges to achieve sustainability, especially in hot dry climate region, such as Jeddah city, KSA, is the urban heat island effect that contributes to energy consumption.  Effat University, Jeddah is striving towards convoying the sustainable trends in developing and designing its campus, in the light of Saudi 2030 vision, which targets reducing energy consumption. This research contributes to the knowledge of rating sustainability through measuring the level of sustainability at Effat University Campus with reference to the LEED v4 rating system (Operation and Maintenance). The research focuses on site selection category, Heat Island Reduction Credit, Option 1 Non-roof Surfaces. This was carried on through quantitative methodology that based on surveying the university campus landscape elements and materials, and interviewing maintenance key persons. The results of this research would potentially help Effat University to improve the campus environmental and energy performance through recommending a set of evidence-driven actions that can help in reducing heat island effect and enhance the efficiency of the university landscape sitting and material. This would also  help in moving Effat University one-step forward to develop a green campus and being LEED Certified

Evaluating Heat Island Effect at University Campus with Reference to LEED v4.

The growing complexity in environmental degradation requires a serious action for adopting sustainable initiatives in the different institutions design and operation. Leadership in Energy and Environmental Design (LEED), is the most widely used green building rating system in the world that helps institutions to evaluate their current sustainable performance, and provides a concise guidance for identifying and implementing practical and measurable solutions. One of the important challenges to achieve sustainability, especially in hot dry climate region, such as Jeddah city, KSA, is the urban heat island effect that contributes to energy consumption.  Effat University, Jeddah is striving towards convoying the sustainable trends in developing and designing its campus, in the light of Saudi 2030 vision, which targets reducing energy consumption. This research contributes to the knowledge of rating sustainability through measuring the level of sustainability at Effat University Campus with reference to the LEED v4 rating system (Operation and Maintenance). The research focuses on site selection category, Heat Island Reduction Credit, Option 1 Non-roof Surfaces. This was carried on through quantitative methodology that based on surveying the university campus landscape elements and materials, and interviewing maintenance key persons. The results of this research would potentially help Effat University to improve the campus environmental and energy performance through recommending a set of evidence-driven actions that can help in reducing heat island effect and enhance the efficiency of the university landscape sitting and material. This would also  help in moving Effat University one-step forward to develop a green campus and being LEED Certified

A Descriptive Study of the Carpal Joint of Healthy Donkeys Using Ultrasonography, Computed Tomography, and Magnetic Resonance Imaging

This study was conducted to establish a detailed anatomic reference for the carpal joint of apparently healthy donkeys using ultrasonography (US), computed tomographic (CT), and magnetic resonance imaging (MRI). Ten orthopedically sound adult donkeys were used for US examination of the carpal joint in each forelimb. Additionally, the carpi of ten donkey cadavers were subjected to CT and MRI examinations. The carpal joint was divided into four zones to simplify examination. US assessment of the carpal joint included transverse and longitudinal sonograms. CT was performed using three planes: axial, sagittal, and coronal. MRI was performed using axial and sagittal planes with two sequences: gradient-echo T1-weighted and proton density. The donkeys’ carpus US, CT, and MRI images were labeled and serially interpreted based on references and anatomical cross-sections. The anatomical characteristics of the carpal joint and the surrounding soft tissue structures were thoroughly described and precisely differentiated on US, CT, and MRI scans. It can be concluded that US, CT, and MRI are effective noninvasive diagnostic imaging tools for evaluating the carpal joint in donkeys. Moreover, these imaging modalities can aid in establishing a reference database for the carpal joint of donkeys, which differs from that of horses

Prevalence and Characterization of PVL-Positive <i>Staphylococcus aureus</i> Isolated from Raw Cow’s Milk

The present study aimed to investigate the prevalence, antibiotic susceptibility profiles, and some toxin genes of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus (S. aureus) in unpasteurized raw cow’s milk collected from retail outlets located at Mansoura, Dakahliya governorate, Egypt. In that context, a total of 700 raw cow’s milk samples were investigated for the presence of S. aureus, which was identified in 41.1% (288/700) of the samples. Among the S. aureus isolates, 113 PVL-positive S. aureus were identified and subjected for further analysis. The PVL-positive S. aureus were investigated for the existence of toxin-related genes, including hemolysin (hla), toxic shock syndrome toxin-1 (tst), and enterotoxins (sea, seb, sec, see, seg, sei, and selj). Genotypic resistance of PVL-positive strains was performed for the detection of blaZ and mecA genes. Among the PVL-positive S. aureus, sea, seb, and sec were detected in 44.2, 6.2%, and 0.9%, respectively, while the hla and tst genes were identified in 54.9% and 0.9%, respectively. The blaZ and mecA genes were successfully identified in 84.9 (96/113) and 32.7% (37/113) of the total evaluated S. aureus isolates, respectively. PVL-positive S. aureus displayed a high level of resistance to penicillin, ampicillin, and trimethoprim-sulfamethoxazole. Multidrug resistance (resistant to ≥3 antimicrobial classes) was displayed by all methicillin-resistant S. aureus (MRSA) and 38.2% of methicillin-sensitive S. aureus (MSSA) isolates. The obtained findings are raising the alarm of virulent PVL-positive MRSA clones in retail milk in Egypt, suggesting the requirement for limiting the use of β-lactam drugs in food-producing animals and the importance of implementing strong hygiene procedures in dairy farms and processing plants

Data_Sheet_1_Molecular identification of Haemonchus contortus in sheep from Upper Egypt.ZIP

IntroductionHaemonchus spp. are considered the most important strongylid nematodes with a worldwide distribution. The parasite’s blood-sucking nature can lead to severe anemia in infected animals. Despite its widespread impact, there is a dearth of comprehensive data on morphological and molecular identification methods for Haemonchus spp. in sheep from Upper Egypt. To address this gap, our current study aimed to assess the prevalence of Haemonchus spp. in 400 sheep fecal samples.MethodsWe employed microscopic examination and molecular techniques, using polymerase chain reaction (PCR) targeting the 18S gene for precise identification. Additionally, the potential risk factors associated with the infection by the parasite in sheep were explored.ResultsThe study pointed out that 33.00% (132 of 400) of the examined sheep were infected with Haemonchus spp. Sheep age and seasonal variability were found to be significant factors (p  0.05) was found between the sex of the examined sheep and the occurrence of haemonchosis. Employing molecular methods, we isolated and identified the parasite through PCR analysis of cultured larvae, which were then subsequently confirmed as Haemonchus contortus via phylogenetic analysis.DiscussionThe study concluded that there was a relatively high occurrence of H. contortus among sheep from Upper Egypt. We recommend the implementation of stringent and effective control measures to combat the infection and safeguard livestock health.</p