Antitumor activity of the tea polyphenol epigallocatechin-3-gallate encapsulated in targeted vesicles after intravenous administration

The therapeutic potential of epigallocatechin gallate, a green tea polyphenol with anti-cancer properties, is limited by its inability to specifically reach tumors following intravenous administration. The purpose of this study is to determine whether a tumor-targeted vesicular formulation of epigallocatechin gallate would suppress the growth of A431 epidermoid carcinoma and B16-F10 melanoma in vitro and in vivo. Transferrin-bearing vesicles encapsulating epigallocatechin gallate were intravenously administered to mice bearing subcutaneous A431 and B16-F10 tumors. The intravenous administration of epigallocatechin gallate encapsulated in transferrin-bearing vesicles resulted in tumor suppression for 40% of A431 and B16-F10 tumors. Animal survival was improved by more than 20 days compared to controls. Encapsulation of epigallocatechin gallate in transferrin-bearing vesicles is a promising therapeutic strategy

Tumor regression following intravenous administration of a tumor-targeted p73 gene delivery system

The potential of gene therapy to treat cancer is hampered by the lack of safe and efficacious gene delivery systems able to selectively deliver therapeutic genes to tumors by intravenous administration. With the long-term aim of developing an efficacious cancer-targeted gene medicine, we demonstrated that transferrin-bearing polypropylenimine dendrimer complexed to a plasmid DNA encoding p73 led to an enhanced anti-proliferative activity in vitro, by up to 120-fold in A431 compared to the unmodified dendriplex. In vivo, the intravenous administration of this p73-encoding dendriplex resulted in a rapid and sustained inhibition of tumor growth over one month, with complete tumor suppression for 10 % of A431 and B16-F10 tumors and long-term survival of the animals. The treatment was well tolerated by the animals, with no apparent signs of toxicity. These results suggest that the p73-encoding tumor-targeted polypropylenimine dendrimer should be further explored as a therapeutic strategy for cancer therapy

Fat mixture and fatty acid composition (% of FAs) of the experimental lipid blends containing increasing levels of caprylic acid (C8:0).

<p>The three different lipid mixes were used for preparing the MF, CR and HF diets.</p><p>¹ΣSFA, ΣMUFA and ΣPUFA correspond respectively to the sum of saturated, mono-unsaturated and poly-unsaturated fatty acids.</p><p>Fat mixture and fatty acid composition (% of FAs) of the experimental lipid blends containing increasing levels of caprylic acid (C8:0).</p

Effect of tricaprylin on body weight gain and food intake in the MF groups.

<p>(<b>a</b>) Body weight gain in gram. Two-way repeated measurement ANOVA: effect of the diet, P = 0.5275. (<b>b</b>) Final body weight in gram; Kruskal-Wallis (K-W) test: P = 0.9098. (<b>c</b>) Cumulative energy intakes in kcal. K-W test: P = 0.3393. Results are expressed as mean ± SEM.</p

Primers designed for quantitative RT-PCR analysis of rat genes.

<p>Primers designed for quantitative RT-PCR analysis of rat genes.</p

Effect of tricaprylin on total ghrelin expression in the MF groups.

<p>(<b>a</b>) Preproghrelin mRNA level (in fold change of the control MF-0) in the stomach tissue. Kruskal-Wallis (K-W) test: P = 0.004, Dunn’s post tests, *P<0.05, **P<0.01. (<b>b</b>) Quantification of total ghrelin staining in the gastric mucosa of the corpus. K-W test: P = 0.017, Dunn’s post tests, **P<0.01. (<b>c</b>) Ghrelin staining in gastric mucosa of MF-0, MF-8 and MF-21 groups.</p

Effect of C8:0 in the MF groups on liver IGF-1 expression.

<p>IGF-1 mRNA level (in fold change). K-W test, P = 0.009, Dunn’s post tests, *P<0.05. Results are expressed as mean ± SEM.</p

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<p>(<b>a</b>) Acylated and unacylated ghrelin concentrations after 2 weeks of diet. Kruskal-Wallis test (K-W): (i) acylated ghrelin, P = 0.0837; (ii) unacylated ghrelin, P = 0.038, Dunn’s post tests, *P<0.05; (iii) total ghrelin, P = 0.2345. (<b>b</b>) Acylated and unacylated ghrelin concentrations after 4 weeks of diet. K-W test: (i) acylated ghrelin, P = 0.4585; (ii) unacylated ghrelin, P = 0.5643; (iii) total ghrelin, P = 0.65. (<b>c</b>) Acylated/total ghrelin ratios after 2 weeks of diet. K-W test: P = 0.0181, Dunn’s post tests, *P<0.05. (<b>d</b>) Acylated/total ghrelin ratios after 4 weeks of diet. K-W test: P = 0.3635.</p

Effect of dietary tricaprylin level on C8:0 availability in the stomach tissue in the MF groups.

<p>The stomach tissue was dissected and the fatty acids of the fundus and corpus were analyzed. The C8:0 available in the different parts of the stomach is expressed as mean ± SEM in % of FAs in the tissues. The effect of the diet and the localization in the stomach tissue was analyzed using two-way ANOVA: (i) effect of the diet on C8:0 availability, P<0.0001; (ii) effect of the part of the stomach tissue studied on C8:0 availability, P = 0.0092; (iii) interaction between the two factors, P = 0.0387.</p

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Recherches Le transfert des exploitations agricoles devient un objectif significatif du XXIe siècle dans plusieurs pays industrialisés en général et au Canada en particulier. Cinq années après leur transfert, quels sont les impacts financiers et technico-économiques sur la performance de ces exploitations au Québec ? La France est l'un des principaux consommateurs de pesticides au monde. Le conseil technique aux agriculteurs peut-il jouer un rôle important dans l'adoption de pratiques économes en pesticides et relever le défi du Grenelle de l'environnement ? L'intérêt pour le commerce transfrontalier des produits agricoles et horticoles entre le Cameroun et ses voisins est évident. Mais la connaissance de son ampleur, de ses déterminants et de ses conséquences reste insuffisante. Lever cette méconnaissance et formuler des recommandations politiques et économiques sont essentiels pour revitaliser cette région. Etat de l'art Dans la gestion quantitative de la ressource en eau souterraine, l’importante littérature modélisée reste éloignée de la réalité. Une autre approche est proposée pour une exploitation maximale de la ressource. Faits et Chiffres À partir des données du RICA, la dispersion du revenu des viticulteurs. Note de lecture Les mondes agricoles en politiqu