Additional file 1: of Humoral response in experimental autoimmune encephalomyelitis targets neural precursor cells in the central nervous system of naive rodents

Methods. Experimental groups, EAE induction and evaluation, Cell culture protocol, Preparation of samples for SDS-PAGE. (RTF 3.45 kb

Additional file 2: Figure S1. of Humoral response in experimental autoimmune encephalomyelitis targets neural precursor cells in the central nervous system of naive rodents

Evidence of the production of anti-MOG-immunoglobulins within antisera when mice were inoculated with MOG peptide. (A) Purified EAE-AS (IgG from EAE-AS) and unpurified EAE-AS identified bands of the same molecular weight on NPCs substrate. Western blot of recombinant MOG as SDS-PAGE substrate (B) and ICC of EL4-MOG cells (C) revealed the real existence of anti-MOG-immunoglobulins within EAE-AS. One band was yielded (above 20kDA) when recombinant MOG was probed with EAE-AS and anti-MOG (positive control) (B). EAE-AS and anti-MOG showed high levels of binding on EL4-MOG cells (DAB staining), whereas NAIVE-AS did not bind (C). Magnification=40X, Scale=100Οm. (JPEG 344 kb