The pooled sensitivity and specificity of unstimulated CSF IFN-γ for diagnosing tuberculous meningitis.

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Hierarchical summary receiver operating characteristic (HSROC) plot to summarize diagnostic accuracy for CSF IGRA in diagnosing tuberculous meningitis.

Hierarchical summary receiver operating characteristic (HSROC) plot to summarize diagnostic accuracy for CSF IGRA in diagnosing tuberculous meningitis.</p

Quality for the included articles.

Quality for the included articles.</p

Articles’ selection process.

Articles’ selection process.</p

Diagnostic performance of IFN-γ in the individual studies.

Diagnostic performance of IFN-γ in the individual studies.</p

The pooled sensitivity and specificity of CSF IGRA for diagnosing tuberculous meningitis.

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Pax6 mRNA expressed pattern during eyelid development.

<p>(A) Semi-quantitative PCR for total expression levels of Pax6 alone and Pax6 and Pax6(5a) using eyelid RNA from E14.5 to E16.5. Pax6 and Pax6(5a) are amplified by the same pair of primers. (B, C) The relative fold expressions of total Pax6 and Pax6(5a) only by real-time PCR, respectively. The reference sample is the cDNA from wild-type eyelids at E14.5. GAPDH is a reference gene. The significantly different data groups at different time point are analysed by Student’s t-test. One asterisk stands for p<0.05, two asterisks mean p<0.01, and no asterisk means no significant difference.</p

The examination of the EGFR-ERK pathway and Smad3 activation in eyelid epidermis at E15.5.

<p>(A–D) The activation state of EGFR and its downstream signalling molecules in the epidermis of wild-type and EOB-5 eyelids. Phosphorylated EGFR and ERK were predominantly stained in wild-type epidermis (A, C), while this staining was weak in the mutant eyelid (B, D). (E, F) The activation state of Smad3. Phosphorylated Smad3 was strongly expressed in the mesenchyme underlying the epidermis of wild-type eyelids (E) and was weakly expressed in the mesenchyme of EOB-5 eyelids (F). (G, H) The examination of CTCF expression. A positive signal (green) was shown in the epidermis of the wild-type eyelid (G), while no positive staining was shown in the mutant eyelid (H). (A’–H’) Negative controls (no primary antibody). Key: ep, epidermis; c, conjunctiva; m, mesenchyme; p, periderm. Bar, 100 µm; arrows indicate positive staining.</p

Immunohistochemistry on eyelid sections from E14.5 to E16.5 with anti-PAX6 antibody.

<p>(A–C) Pax6 staining in wild-type sections. There is weak but clear staining shown in the conjunctiva and mesenchyme at E14.5 (A); little positive staining is shown in the mesenchymal cells, none is in the epidermis at E15.5 (B), and Pax6 is only in peridermal cells at E16.5. (D–F) Pax6 staining of EOB-5 eyelid sections. There is intense staining in epidermis and mesenchyme at all three days. (A’–F’) Negative controls without primary antibody. Key: ep, epidermis; c, conjunctiva; m, mesenchyme; p, periderm. Bar, 100 µm; arrows indicate positive staining.</p

Cell migration in wild-type and mutant eyelids from E14.5 to E16.5.

<p>F-actin polymerisation is shown at the margin of the eyelid epithelium in both wild-type (A) and EOB-5 (D) embryos at E14.5. An intense positive staining is shown in the epithelial layer of wild-type (B) and EOB-5 (E) eyelids at E15.5. F-actin polymerisation was reduced when eyelid fusion was completed. More intense staining of polymerised F-actin was detected in the wild-type (C) embryo at E16.5 and in the un-fused eyelid of EOB-5 (F) embryo at E16.5. (A’–F’) Negative controls without phalloidin. Rhodamine-phalloidin was used to visualise F-actin (red). Bar, 100 µm.</p