TEM images of the hippocampus in the SPS rats.

<p>TEM images of the hippocampus. A: Normal control hippocampal neuron, displaying abundant organelles such as mitochondria, endoplasmic reticulum, and ribosomes. The nucleus is large and round, density of chromatin is uniform, and nucleolus is clear. B: Chromatin margination, in the form of crescent formation in nucleus (arrow), is shown. C: The cells showed some typical structural changes indicative of apoptosis, such as chromatin dissolution and, nuclear envelope breakdown (star). Scale bar = 200 nm (A). Scale bar = 300 nm (B and C).</p

Western blot of GRP78 in the hippocampus of SPS rats.

<p>GRP78 protein expression (A) and results from its quantitative analysis based on western blot results (B). GRP78 expression was analyzed in the hippocampus of control rats and of rats subjected to single prolonged stress (SPS). An increase in GRP78 protein expression was observed in SPS rats. *P<0.05 vs. the control group.</p

RT-PCR of GRP78 in the hippocampus of the SPS rats.

<p>GRP78 mRNA expression (A) and results from its quantitative analysis (B). GRP78 mRNA expression in the hippocampus of rats subjected to SPS was higher than that in the hippocampus of control rats. *P<0.05 vs. the control group.</p

RT-PCR of Caspase-12 in the hippocampus of the SPS rats.

<p>Caspase-12 mRNA expression (A) and results from its quantitative analysis (B). Caspase-12 mRNA level in the hippocampus of rats subjected to SPS was higher than that in the hippocampus of control rats. *P<0.05 vs. the control group, #P<0.05 vs. the SPS 1 day group.</p

Change of intracellular calcium levels in the hippocampal cells of the SPS rats.

<p>Image of intracellular calcium levels in the control group (A), 1 day (B) and 4 days(C) after SPS and change in intracellular calcium (Ca²⁺) levels (nmol/l) from its quantitative analysis. *P<0.05 vs. the control group.</p

Western blot of caspase-12 in the hippocampus of SPS rats.

<p>Caspase-12 protein expression in the endoplasmic recutilum (ER), cytoplasm (Cyto) and mitochondira (Mito) fractions of the hippocampal cells (A) and results from its quantitative analysis based on western blot results (B). An increase in caspase-12 protein expression in the ER was observed in SPS rats.*P<0.01 vs. the control group.</p

All primers for RT-PCR.

<p>All primers for RT-PCR.</p

Classification performance of each machine learning model.

Classification performance of each machine learning model.</p

An example of the highly cited patent identification indicator system.

An example of the highly cited patent identification indicator system.</p

Derivation procedure for each index.

Derivation procedure for each index.</p