An integrated chromatin accessibility and transcriptome landscape of human pre-implantation embryos

Early human embryonic development involves extensive changes in chromatin structure and transcriptional activity. Here the authors present LiCAT-seq, a method enabling simultaneous profiling of chromatin accessibility and gene expression with ultra-low input of cells and map chromatin accessibility and transcriptome landscapes for human pre-implantation embryos

Analysis of the current status and comprehensive application research on BIM technology in construction cost management

With the advancement of national economic strategy, the construction process has encountered an escalation in problems and challenges, thereby emphasizing the significance of cost management. The current management of construction costs is confronted with numerous challenges, encompassing limited visualization capabilities, inadequate collaboration among diverse professions, isolated decision-making processes, and a lack of automated procedures. The emergence of BIM technology has brought forth the possibility to address these challenges, offering a green, efficient, and sustainable solution. The comprehensive implementation of BIM technology in cost management enables the refinement of project cost management. By conducting an in-depth analysis of the current state of civil engineering construction cost management, this paper proposes a comprehensive approach to applying BIM technology, which offers scientific and technical means for addressing these issues and serves as a catalyst for generating valuable insights

A Study of PHC Pipe Pile Vertical Ultimate Bearing Capacity Calcula-tion Method and its Numerical Simulation Analysis

According to the PHC pipe pile design of Xinwan Plaza, Huaiyuan, Anhui and pile test results of static sounding method and experience method using the ultimate bearing capacity of single pile are calculated, considering the effect of soil plug the influence of vertical ultimate bearing capacity of single pile, and the formula for calculating the lateral resistance and end resistance, static sounding method and the correction formula to calculate the bearing capacity calculated results was coincident with the results of test pile. Combining with the project of Xinwan Plaza, Huaiyuan, Anhui, through the static load test, a study of the load transfer mechanism of PHC pipe pile, analyzes the vertical load, the change rule of the settlement of pile body and pile and the use of finite difference software FLAC3D simulation of the PHC pipe pile static load test, the simulation results agreed with the measured load-settlement curve

Design and Construction Method of High-low Span Cross Support System for Frame Structures

Taking a high-rise frame-shear wall structure project in Jilin Province as an example, combined with current specifications, standards, and regulations, this paper proposes the design and construction method of the scaffold support system for the high-low span and high formwork between the basement and the main structure；In the construction, the side formwork of the high-low span beam adopts the method of suspending formwork, and the combination of fixing support and drawing bolts on both sides. The floor slab is poured first, followed by the high-low span, and the pouring is carried out in layers and sections, ensuring the quality of the project and shortening the construction period. After construction verification, the various acceptance values are better than the current engineering acceptance evaluation standard values, and it is safe and reliable. This provides a reference for the design and construction of similar high-low span and high-formwork projects in frame structures

Development of nanomodified eco-friendly thermal energy storing cementitious composite using PCM microencapsulated in biosourced encapsulation shell

This study presents the development of a thermal energy storing cementitious composite that incorporates phase change material (PCM) microencapsulated in biosourced polymer shell (m-PCM). The biodegradability of the encapsulated PCM was assessed using Nuclear Magnetic Resonance (NMR), which confirmed the high biodegradability of the m-PCM shell under specific environmental conditions. To examine the thermophysical properties of the m-PCM, Differential Scanning Calorimetry (DSC) and Thermogravimetric analysis (TGA) were performed. The DSC results revealed exothermic enthalpies of 84.7 J/g and 140.2 J/g, with associated peak temperatures of − 3.2 °C and − 22.9 °C, respectively. Subsequently, the m-PCM was incorporated into cement-based mortar at proportions of 5%, 10%, and 15% by weight of binder. Uniaxial compression tests were conducted to evaluate the effect of the m-PCM on the mechanical properties of the mortar. The results indicated a significant decrease in mechanical strength upon incorporating the m-PCM. However, this reduction in strength was mitigated by the addition of silica fume (SF) and multiwalled carbon nanotubes (MWCNTs) in combination. Furthermore, a thermal cycling test was performed to examine the behavior of the nanomodified m-PCM incorporated mortar under varying ambient conditions. The results showed that the addition of MWCNTs improved both the mechanical performance and thermal performance of the composite

Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana

Background. Water channel proteins, also called aquaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch. & C.A. Mey (C. bungeana) are still unknown. Results. In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C. bungeana. Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus. Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C. bungeana. Conclusion. This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C. bungeana. Our findings contribute to understanding the water transports in plants under low temperatures