Venn diagram of annotation results against Nr, SwissProt, COG and KEGG databases.

<p>The number in each color block indicates the number of unigenes that is annotated by single or multiple databases.</p

Characteristics of 16 polymorphic microsatellite loci.

<p>Ho, observed heterozygosity; HE, expected heterozygosity; PHWE, Hardy-Weinberg probability; PIC, polymorphic information content.</p><p>Characteristics of 16 polymorphic microsatellite loci.</p

Frequency of classified repeat types of SSRs.

<p>Frequency of classified repeat types of SSRs.</p

Statistics of the assembled transcripts and unigenes.

<p>Statistics of the assembled transcripts and unigenes.</p

Length distribution of all unigenes of <i>Trachinotus ovatus</i>.

<p>Length distribution of all unigenes of <i>Trachinotus ovatus</i>.</p

Top-hit species distribution for sequences from <i>Trachinotus ovatus</i> submitted BLASTX against the NCBI-Nr database.

<p>Only fish species were present in this study.</p

Comparison of <i>Trachinotus ovatus</i> unigenes to <i>Danio rerio</i> orthologous coding sequences.

<p>(A) The ratio of <i>Trachinotus ovatus</i> unigene length to <i>Danio rerio</i> ortholog length was plotted against <i>Trachinotus ovatus</i> unigene coverage depth. (B) The total percentage of <i>Danio rerio</i> ortholog coding sequence covered by all <i>Trachinotus ovatus</i> unigenes.</p

Distribution of identified SSRs using the MISA software.

<p>Distribution of identified SSRs using the MISA software.</p

Gene Ontology (GO) analysis and functional classification of the <i>Trachinotus ovatus</i> transcriptome.

<p>Gene Ontology (GO) analysis and functional classification of the <i>Trachinotus ovatus</i> transcriptome.</p