原条から体節中胚葉へ : 初期発生段階のニワトリ胚のEGFP遺伝子導入による標識

内容の要旨 , 審査の要旨広島大学(Hiroshima University)博士(医学)Doctor of Philosophy in Medical Sciencedoctora

Strain Rockbursts Simulated by Low-Strength Brittle Equivalent Materials

This paper presents experimental study on rockbursts that occur in deep underground excavations. To begin with, the boundary conditions for excavation in deep underground engineering were analysed and elastic adaptive boundary is an effective way to minimize the boundary effect of geomechanical model test. Then, in order to simulate an elastic adaptive loading boundary, Belleville springs were used to establish this loading boundary. With the aforementioned experimental set-ups and fabrication of similarity models for test, the phenomena of strain mode rockbursts were satisfactorily reproduced in laboratory. The internal stress, strain, and convergences of the openings of the model were instrumented by subtly preembedded sensors and transducers. Test results showed that, with an initial state of high stress from both upper layers’ gravitational effects and in situ stress due to tectonic movements, the excavation brings a dramatic rise in the hoop stress and sharp drop in radial stress, which leads to the splitting failure of rock mass. Finally a rockburst occurred associated with the release of strain energy stored in highly stressed rock mass. In addition, the failure of the surrounding rock demonstrated an obvious hysteresis effect which supplies valuable guide and reference for tunnel support. Not only do these results provide a basis for further comprehensive experiments, but also the data can offer assisting aids for further theoretical study of rockbursts

MAKET KRATON PLERED CIRCA : 1675

Maket ini berusaha mengangkat suatu program yang subjeknya cukup menantang yakni, Kraton Plered yang keberadaannya sekarang sudah hancur rata dengan tanah. Pertimbangan kurikuler historis didaktis tetap menjadi acuan seleksi subjek model dan dilakukan secara objektif saintifik, Tentatif Historis Imajinasi Berdasarkan Kesaksian Sejarah Tujuan Penelitian ini adalah sebuah analisis historis pertumbuhan dan perkembangan Kraton Plered serta merupakan visualisasi Kraton Plered beserta lingkungan sekitarnya pada masa Kasultanan Amangkurat Agung (I). Penelitian ini menggunakan metode sejarah yakni, diawali dari penentuan subjek, perumusan problema, survai, pengumpulan sumber, analisa sumber, kesimpulan sementara dan berakhir dengan seminar umum, follow up hasil seminar, disimpulkan dalam bentuk grafis (peta, diagram, Chart, denah, irisan, tampak depan, samping, dan atas) "BLUEPRINT". Berdasarkan blueprint, kerja fisik pembuatan maket dilakukan, yang meliputi langkah memotong, merakit, memasang dan finishing. Hasil penelitian merupakan rekonstruksi sebuah model tiga dimensi berupa maket Kraton Plered beserta kelengkapannya, seperti ilustrasi, fotografi, poster, peta, makalah, bibliografi, dokumen, audio visual, dan sebagainya. Kesemuanya untuk sementara disimpan di Ruang Disply Laboratorium Sejarah, FKIP Unversitas PGRI Yogyakarta, Kampus Unit I lantai I gedung B

Label-free visualization of carbapenemase activity in living bacteria

Evaluating enzyme activity intracellularly on natural substrates is a significant experimental challenge in biomedical research. We report a label‐free method for real‐time monitoring of the catalytic behavior of class A, B, and D carbapenemases in live bacteria based on measurement of heat changes. By this means, novel biphasic kinetics for class D OXA‐48 with imipenem as substrate is revealed, providing a new approach to detect OXA‐48‐like producers. This in‐cell calorimetry approach offers major advantages in the rapid screening (10 min) of carbapenemase‐producing Enterobacteriaceae from 142 clinical bacterial isolates, with superior sensitivity (97 %) and excellent specificity (100 %) compared to conventional methods. As a general, label‐free method for the study of living cells, this protocol has potential for application to a wider range and variety of cellular components and physiological processes

Ultrasensitive (Co)polymers Based on Poly(methacrylamide) Structure with Fining-Tunable pH Responsive Value

Novel pH responsive copolymers with tertiary amine groups were prepared by free radical polymerization with 2-(dialkylamino)ethyl methacrylate monomers. These polymers were pH sensitive with the ability to be responsively fine-tuned in aqueous solution, which was proven through titration, transmittance measurements, and proton nuclear magnetic resonance spectroscopy. The polymers were soluble in water at low pH values, induced by electrostatic repulsion between amine groups, and aggregated above their pKa value due to the hydrophobic effect of the alkyls. The pH responsive values were precisely tuned from 7.4 to 4.8 by increasing the hydrophobic monomer ratio. Our work provides a novel approach for the development of ultrasensitive pH-responsive polymers for application in biomedical materials

Rheology Properties and Plugging Performance of Fluorescent Polyacrylamide Microspheres in Fractures

Polyacrylamide microspheres have recently drawn great attention in conformance control due to their advantages over traditional gel treatments. One important question that has been raised is whether the tiny particles can be produced from production wells. However, current products are difficult to use for detecting fluids generated by production wells. In this paper, the fluorescent polyacrylamide microspheres were successfully synthesized; they can emit blue fluorescence under ultraviolet irradiation. Their swelling property, fluorescence characteristics, rheology property, creep-recovery property, and plugging performance were evaluated in the laboratory. The results indicated that the microspheres could emit blue fluorescence under ultraviolet irradiation after passing through a fracture model. Their creep-recovery ability after deformation was very high, and their elastic recovery rate extended to 94.1%, which suggests that the microspheres almost recover to their original shape and size after deformation. The transparent fracture model plugging test shows that the microspheres can migrate and plug a fracture with a width of 0.3 mm, which is much smaller than the diameter of the microspheres