Vehicle identification from automotive paints transferred in road accidents

Abstract in proceedings of the Fourth International Congress of CiiEM: Health, Well-Being and Ageing in the 21st Century, held at Egas Moniz’ University Campus in Monte de Caparica, Almada, from 3–5 June 2019.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/publishedVersio

Relationship between body composition, sprint performance and vertical jump tests in young elite soccer players

Abstract of poster presented at the 2nd International Congress of CiiEM - Translational Research and Innovation in Human and Health Science. 11-13 June, 2017, Monte de Caparica, Portugalinfo:eu-repo/semantics/publishedVersio

Relationship between body composition, sprint performance and vertical jump tests in young elite soccer players

Poster presented at the 2nd International Congress of CiiEM - Translational Research and Innovation in Human and Health Science. 11-13 June 2017, Campus Egas Moniz, Caparica, PortugalN/

The influence of intraoral devices on dynamic performance of golf athletes, a pilot study

Poster presented at the 2nd International Congress of CiiEM - Translational Research and Innovation in Human and Health Science. 11-13 June, 2017, Campus Universitário Egas Moniz, Monte de Caparica, PortugalN/

Evolutionary and Structural Features of the C2, V3 and C3 Envelope Regions Underlying the Differences in HIV-1 and HIV-2 Biology and Infection

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Users must also make clear the license terms under which the work was published.Background: Unlike in HIV-1 infection, the majority of HIV-2 patients produce broadly reactive neutralizing antibodies, control viral replication and survive as elite controllers. The identification of the molecular, structural and evolutionary footprints underlying these very distinct immunological and clinical outcomes may lead to the development of new strategies for the prevention and treatment of HIV infection.Methodology/Principal Findings: We performed a side-by-side molecular, evolutionary and structural comparison of the C2, V3 and C3 envelope regions from HIV-1 and HIV-2. These regions contain major antigenic targets and are important for receptor binding. In HIV-2, these regions also have immune modulatory properties. We found that these regions are significantly more variable in HIV-1 than in HIV-2. Within each virus, C3 is the most entropic region followed by either C2 (HIV-2) or V3 (HIV-1). The C3 region is well exposed in the HIV-2 envelope and is under strong diversifying selection suggesting that, like in HIV-1, it may harbour neutralizing epitopes. Notably, however, extreme diversification of C2 and C3 seems to be deleterious for HIV-2 and prevent its transmission. Computer modelling simulations showed that in HIV-2 the V3 loop is much less exposed than C2 and C3 and has a retractile conformation due to a physical interaction with both C2 and C3. The concealed and conserved nature of V3 in the HIV-2 is consistent with its lack of immunodominancy in vivo and with its role in preventing immune activation. In contrast, HIV-1 had an extended and accessible V3 loop that is consistent with its immunodominant and neutralizing nature.Conclusions/Significance: We identify significant structural and functional constrains to the diversification and evolution of C2, V3 and C3 in the HIV-2 envelope but not in HIV-1. These studies highlight fundamental differences in the biology and infection of HIV-1 and HIV-2 and in their mode of interaction with the human immune system and may inform new vaccine and therapeutic interventions against these viruses.Fundo para a Ciência e Tecnologia, Portugal. Collaborative HIV and Anti-HIV Drug Resistance Network, European Union. PB and IB supported by PhD grants from Fundo para a Ciência e Tecnologia

The influence of intraoral devices in competitive anxiety levels of golf athletes : a pilot study

Poster presented at the 3rd International Congress of CiiEM - Research and Innovation in Human and Health Sciences. Campus Egas Moniz, Monte da Caparica, Portugal, 20-22 June 2018N/

Insulin glycation by methylglyoxal results in native-like aggregation and inhibition of fibril formation

<p>Abstract</p> <p>Background</p> <p>Insulin is a hormone that regulates blood glucose homeostasis and is a central protein in a medical condition termed insulin injection amyloidosis. It is intimately associated with glycaemia and is vulnerable to glycation by glucose and other highly reactive carbonyls like methylglyoxal, especially in diabetic conditions. Protein glycation is involved in structure and stability changes that impair protein functionality, and is associated with several human diseases, such as diabetes and neurodegenerative diseases like Alzheimer's disease, Parkinson's disease and Familiar Amyloidotic Polyneuropathy. In the present work, methylglyoxal was investigated for their effects on the structure, stability and fibril formation of insulin.</p> <p>Results</p> <p>Methylglyoxal was found to induce the formation of insulin native-like aggregates and reduce protein fibrillation by blocking the formation of the seeding <it>nuclei</it>. Equilibrium-unfolding experiments using chaotropic agents showed that glycated insulin has a small conformational stability and a weaker dependence on denaturant concentration (smaller m-value). Our observations suggest that methylglyoxal modification of insulin leads to a less compact and less stable structure that may be associated to an increased protein dynamics.</p> <p>Conclusions</p> <p>We propose that higher dynamics in glycated insulin could prevent the formation of the rigid cross-β core structure found in amyloid fibrils, thereby contributing to the reduction in the ability to form fibrils and to the population of different aggregation pathways like the formation of native-like aggregates.</p

The effect of endurance training in salivary flow and pH

Poster presented at the 3rd International Congress of CiiEM - Research and Innovation in Human and Health Sciences. Campus Egas Moniz, Monte da Caparica, Portugal, 20-22 June 2018N/

Dental caries incidence in a sample of endurance sports athletes

Poster presented at the 3rd International Congress of CiiEM - Research and Innovation in Human and Health Sciences. Campus Egas Moniz, Monte da Caparica, Portugal, 20-22 June 2018N/

The influence of intraoral devices on static performance in golf athletes : pilot study

Abstract of the poster presented at the 2nd International Congress of CiiEM - Translational Research and Innovation in Human and Health Science. 11-13 June, 2017, Monte de Caparica, Portugalinfo:eu-repo/semantics/publishedVersio