Polyamines in cell culture

Described is an investigation into the role of polyamines in plant cells. The study was performed throughout with the use of a liquid suspension culture of carrot (Daucus carota cv Chantenay) cells as the model system. Polyamine synthesis and accumulation were studied during the culture growth period, with biosynthetic and catabolic enzyme assays performed to determine rates of polyamine synthesis and free-polyamine analysis to determine cellular polyamine levels. Methods of manipulating polyamine levels within the cell were studied. These included varying the constituents of the growth medium of the suspension culture, such as the plant hormone levels and the balance and level of the available nitrogen sources. The possibility that polyamine supplements might replace plant hormones in the growth medium was investigated and polyamine precursors were added to the medium in an attempt to boost cellular polyamine levels. A substantial comparative study was also made into the effects of inhibitors of polyamine biosynthesis on polyamine biosynthesis and accumulation. Included were determinations of the effects of a range of inhibitors on polyamine biosynthetic and catabolic enzyme activities, on the accumulation of polyamines within the cell and on growth over the culture period. Finally, the relationship between polyamines and other solutes, and cellular responses to short and long-term stress conditions was studied - both in cells previously adapted to stress conditions, and unadapted cell lines. Principal findings were: a) Difluoromethylarginine strongly inhibits arginine decarboxylase and radically reduces putrescine and total amine content, while having no effect on cell number and stimulating fresh weight through increased cell expansion. b) Difluoromethylornithine strongly inhibits ornithine decarboxylase activity but has little effect on either polyamine levels or culture growth. c) Ethylmethylglyoxalbisguanylhydrazone strongly inhibits S- adenosylmethionine decarboxylase activity with little effect on fresh weight. d) Proline and other organic solutes increase in concentration in response to polyethylene glycol induced water stress in adapted and unadapted cell lines while polyamines and glycinebetaine do not increase significantly

A Probabilistic Deformation Demand Model and Fragility Estimates for Asymmetric Offshore Jacket Platforms

Interest in evaluating the performance and safety of offshore oil and gas platforms has been expanding due to the growing world energy supply and recent offshore catastrophes. In order to accurately assess the reliability of an offshore platform, all relevant uncertainties must be properly accounted for. This necessitates the development of a probabilistic demand model that accounts for the relevant uncertainties and model errors. In this study, a probabilistic demand model is developed to assess the deformation demand on asymmetric offshore jacket platforms subject to wave and current loadings. The probabilistic model is constructed by adding correction terms and a model error to an existing deterministic deformation demand model. The correction terms are developed to capture the bias inherent in the deterministic model. The model error is developed to capture the accuracy of the model. The correction terms and model errors are estimated through a Bayesian approach using simulation data obtained from detailed dynamic analyses of a set of representative asymmetric offshore platform configurations. The proposed demand model provides accurate and unbiased estimates of the deformation demand on offshore jacket platforms. The developed probabilistic demand model is then used to assess the reliability of a typical offshore platform considering serviceability and ultimate performance levels. In addition, a sensitivity analysis is conducted to assess the effect of key parameters on the results of the analyses. The proposed demand model can be used to assess the reliability of different design options and for the reliability-based optimal design of offshore jacket platforms

A comparison of Charolais and beef X Friesian suckler cows.

End of Project ReportThe studies carried out included comparisons of Charolais and Beef x Friesian suckler cows in terms of voluntary silage intake, colostrum yield and immunoglobulin level, calf immunoglobulin level and cow milk yield in addition to animal production experiments. In all experiments the Charolais animals used were a minimum of 7/8 Charolais and were the result of an upgrading programme at Grange commencing with Charolais x Friesians. In the production experiments, only Hereford x Friesian cows (and their progeny) were compared with the Charolais while in all other experiments the Beef x Friesians included both Hereford x Friesians and Limousin x Friesians.European Union Structural Funds (EAGGF

Production of red veal for the EU market.

End of Project ReportA summary of four experiments which used Holstein/Friesian bulls and a fifth which used continental cross bulls to determine the effect of feeding ad libitum concentrates on animal prefomance. • The Barley soyabean meal ration provided consistent liveweight gain (1.25 kg/day) and similar cold carcass weight (237 kg) in all four experiments using Holstein and Friesian Bulls. • The Low energy treatment group (Experiment 1) had a liveweight gain of 1.14 kg/day and a cold carcass of 220 kg, i.e. 21 kg lower than that achieved on the High energy treatment group which had a liveweight gain of 1.26 kg/day and a cold carcass of 241 kg. • Providing animals with a summer period outdoors at pasture compared with a continuous period indoors, in general had the effect of improving daily liveweight gain and feed conversion efficiency when animals were offered ad libitum concentrate diet. The effect was greatest when autumn-born calves spent the final 180 days prior to slaughter at pasture. • Restricting the concentrate allowance at pasture brought about a 1.5 unit improvement in FCR carcass, however, at a similar slaughter age this treatment group produced carcasses that were 22 and 21 kg lighter than the control in two respective experiments. • The current economic climate for beef production does not permit the production of red veal in Ireland. However, when markets develop in the Mediterranean countries there will be opportunities to produce red veal carcasses for those markets using male bulls from the Holstein/Friesian herds. • Weaned continental cross suckler bulls slaughtered off an ad libitum concentrate diet at 550 kg liveweight had a feed conversion efficiency of 8.2 kg concentrate DM per kg carcass produced. The corresponding value at 650 kg slaughter, liveweight was 9.5 kg.European Union Structural Funds (EAGGF

Quality Suckler Beef From Low and High Input Grassland Management Systems

End of Project ReportSpring calving cows were used in the years 1997 to 2003 in the development of planned low and high input systems of suckler beef production.The main objective of the study was to compare a semiintensive Grange (standard): system of suckler beef production with a more extensive REPS (Rural Environment Protection Scheme) compatible system. In the standard system the stocking rate was 0.80 ha per cow unit (cow plus heifer and steer progeny to slaughter at 20 and 23/24 months of age, respectively, plus replacements or its equivalent), a nitrogenous fertiliser application rate of 210 kg per ha and two silage harvests each year amounting to the equivalent of 87% of the total area harvested. The REPS system involved a 25% lower stocking rate, an annual nitrogen fertiliser application rate of 90 to 100 kg per ha and one silage harvest (portion in late May to provide good silage for the progeny and the remainder in June to provide lower quality silage for cows) amounting to 58% of the total area harvested. Between 1997 and 2000 the cow herd were Limousin x Friesians (LF) and Simmental x (Limousin x Friesians) (SLF). A herd of first calvers were introduced in 2001 and 2002 which in addition to LF and SLF included Limousin x (Limousin x Friesians), purebred Limousin and purebred Charolais. Charolais (or Simmental) sires were used on mature cows. Replacement heifers were bred to calve at 2 years of age using an easy calving Limousin bull. Concentrate inputs per animal were the same in the two systems. The main findings of the study were: • The mean nitrogenous application rates were 210 and 98 kg per ha in the standard and REPS systems, respectively. • Mean dry matter digestibility of the first-cut silages harvested early (May 19 to May 29), late (June 5 to June 13) and the second cut silage in the standard system (harvested in July/early August) were 716, 690 and 674 g/kg, respectively. • When averaged throughout two grazing seasons there was no difference between the standard and REPS systems in pregrazing or post-grazing sward heights. When examined over one grazing season the only major difference between the grazing areas was that the pasture crude protein content was higher in the standard system in both the pre-grazed (205 and 159 g/kg) and post-grazed (172 v 141 g/kg) swards. Cow liveweight and body condition score gains at pasture and calf gains from birth to weaning were the same for both systems. Carcass weights of the progeny were the same for the standard and REPS systems

An Approach to Optmising the Harvesting of Tidal Energy

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv

The Application of a High Resolution Nested Hydrodynamic Model to Quantification of Power Output from Tidal Turbine Arrays

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv

Efficient AUV Navigation Fusing Acoustic Ranging and Side-scan Sonar

This paper presents an on-line nonlinear least squares algorithm for multi-sensor autonomous underwater vehicle (AUV) navigation. The approach integrates the global constraints of range to and GPS position of a surface vehicle or buoy communicated via acoustic modems and relative pose constraints arising from targets detected in side-scan sonar images. The approach utilizes an efficient optimization algorithm, iSAM, which allows for consistent on-line estimation of the entire set of trajectory constraints. The optimized trajectory can then be used to more accurately navigate the AUV, to extend mission duration, and to avoid GPS surfacing. As iSAM provides efficient access to the marginal covariances of previously observed features, automatic data association is greatly simplified — particularly in sparse marine environments. A key feature of our approach is its intended scalability to single surface sensor (a vehicle or buoy) broadcasting its GPS position and simultaneous one-way travel time range (OWTT) to multiple AUVs. We discuss why our approach is scalable as well as robust to modem transmission failure. Results are provided for an ocean experiment using a Hydroid REMUS 100 AUV co-operating with one of two craft: an autonomous surface vehicle (ASV) and a manned support vessel. During these experiments the ranging portion of the algorithm ran online on-board the AUV. Extension of the paradigm to multiple missions via the optimization of successive survey missions (and the resultant sonar mosaics) is also demonstrated.United States. Office of Naval Research (Grant N000140711102

Presynaptic Translation: Stepping Out of the Postsynaptic Shadow

The ability of the nervous system to convert transient experiences into long-lasting structural changes at the synapse relies upon protein synthesis. It has become increasingly clear that a critical subset of this synthesis occurs within the synaptic compartment. While this process has been extensively characterized in the postsynaptic compartment, the contribution of local translation to presynaptic function remains largely unexplored. However, recent evidence highlights the potential importance of translation within the presynaptic compartment. Work in cultured neurons has shown that presynaptic translation occurs specifically at synapses undergoing long-term plasticity and may contribute to the maintenance of nascent synapses. Studies from our laboratory have demonstrated that Fragile X proteins, which regulate mRNA localization and translation, are expressed at the presynaptic apparatus. Further, mRNAs encoding presynaptic proteins traffic into axons. Here we discuss recent advances in the study of presynaptic translation as well as the challenges confronting the field. Understanding the regulation of presynaptic function by local protein synthesis promises to shed new light on activity-dependent modification of synaptic architecture

Pain Management in Cancer Center Inpatients:A Cluster Randomized Trial to Evaluate a Systematic Integrated Approach—The Edinburgh Pain Assessment and Management Tool

Purpose Pain is suboptimally managed in patients with cancer. We aimed to compare the effect of a policy of adding a clinician-delivered bedside pain assessment and management tool (Edinburgh Pain Assessment and management Tool [EPAT]) to usual care (UC) versus UC alone on pain outcomes. Patients and Methods In a two-arm, parallel group, cluster randomized (1:1) trial, we observed pain outcomes in 19 cancer centers in the United Kingdom and then randomly assigned the centers to either implement EPAT or to continue UC. The primary outcome was change in the percentage of study participants in each center with a clinically significant (≥ 2 point) improvement in worst pain (using the Brief Pain Inventory Short Form) from admission to 3 to 5 days after admission. Secondary outcomes included quality of analgesic prescribing and opioid-related adverse effects. Results Ten centers were randomly assigned to EPAT, and nine were assigned to UC. We enrolled 1,921 patients and obtained outcome data from 93% (n = 1,795). Participants (mean age, 60 years; 49% women) had a variety of cancer types. For centers randomly assigned to EPAT, the percentage of participants with a clinically significant improvement in worst pain increased from 47.7% to 54.1%, and for those randomly assigned to continue UC, this percentage decreased from 50.6% to 46.4%. The absolute difference was 10.7% (95% CI, 0.2% to 21.1%; P = .046) and it increased to 15.4% (95% CI, 5.8% to 25.0%; P = .004) when two centers that failed to implement EPAT were excluded. EPAT centers had greater improvements in prescribing practice and in the Brief Pain Inventory Short Form pain subscale score. Other pain and distress outcomes and opioid adverse effects did not differ between EPAT and UC. Conclusion A systematic integrated approach improves pain outcomes for inpatients in cancer centers without increasing opioid adverse effects