34 research outputs found
Characterization of genetic variability among common bean genotypes by morphological descriptors
Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala
Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement
Identificação e quantificação dos componentes de perdas de produção do tomateiro em ambiente protegido
Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans
Identification of candidate genes involved in Witches’ broom disease resistance in a segregating mapping population of Theobroma cacao L. in Brazil
Polymorphic microsatellite loci for Stylosanthes macrocephala Ferr. et Costa, a tropical forage legume
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento CientÃfico e Tecnológico (CNPq)Microsatellites were developed for Stylosanthes macrocephala, aiming at developing tools for studying the genetic diversity of this species. A total of 13 polymorphic microsatellite markers were isolated from a S. macrocephala enriched genomic library. The isolated microsatellites were characterized in 20 accessions of the S. macrocephala germplasm collection belonging to the Empresa Brasileira de Pesquisa Agropecuaria (Embrapa) Cerrados. The number of alleles per locus varied from 2 to 11, with an average of 4 alleles per locus. The observed and expected heterozygosities ranged from 0 to 0.25 and 0.05 to 0.90, respectively. Cross-amplification of the S. macrocephala polymorphic microsatellites was evaluated in three other Stylosanthes species. The microsatellites reported herein are the first set of microsatellite markers developed for S. macrocephala and are potentially useful for further studies on genetic diversity, conservation and breeding of this species.11481485Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento CientÃfico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento CientÃfico e Tecnológico (CNPq)FAPESP [2005/51010-0, 2005/52211-9