494 research outputs found

    Comparison of rapid laboratory tests for failure of passive transfer in the bovine

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    peer-reviewedBackground Failure of passive transfer of maternal immunity via colostrum can occur in the bovine, and a number of blood tests have been developed to test calves for this failure. It is not clear which test is most suitable for this purpose. The objective was to examine the most commonly used tests for failure of passive transfer and to decide which is most suitable for routine laboratory use. 126 serum samples were taken from calves of dairy cows after birth but prior to colostrum feeding, and at 48 h of age. Five different tests were compared against radial immunodiffusion which is considered the appropriate reference method. These tests were serum gamma-glutamyltransferase levels, serum protein levels, serum globulin levels, an enzyme linked immunosorbent assay and the zinc sulphate turbidity test. Results The tests examined displayed high sensitivity but widely varying specificity. Examination of the use of different cut-off points allowed some improvement in specificity at the expense of sensitivity, but the tests which had performed best at the original cut-off points still displayed the best performance. Gamma-glutamyltransferase levels as a measure of colostrum absorption returned, in this study, the best balance between sensitivity and specificity. The ELISA used in this study and serum globulin levels displayed performance similar to the gamma-glutamyltransferase levels. Serum total protein was less successful than others examined at providing both sensitivity and specificity but may, when performed via refractometer, be useful for on-farm testing. As currently performed the poor sensitivity for which the zinc sulphate turbidity test is most often criticized is evident. Modification of the cut-off point to increase specificity is less successful at balancing these parameters than the ELISA, gamma-glutamyltransferase levels, and globulin levels. Conclusions Gamma-glutamyltransferase levels, ELISA testing and circulating globulin levels performed best in detecting failure of passive transfer in serum samples, although all three had some practical considerations

    The Physical and Cultural Background

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    The Sunken Village wet site (35MU4) is on one of the most important river junctures on the Northwest Coast of North America – where the Columbia River drainage of British Columbia, Canada and Washington State, U.S.A. is joined by the Willamette River flowing through much of western Oregon State, U.S.A. (Figure 2.1). The site is on Sauvie Island, where a major aquifer pumps under the natural levee into Multnomah Channel, providing a unique 125 m wide beach area where acorns placed in shallow hemlock bough-lined pits were leached in huge numbers by ancient Multnomah Peoples (Figure 2.2)

    The 2006 and 2007 Field Seasons at Sunken Village

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    As mentioned, the 2005 erosion of the dike placed on the natural levee facing the intertidal waterlogged portion of the Sunken Village site had threatened the residents of the island, initiating a proposal by the Sauvie Island Drainage Improvement Company (SIDIC) to place protective rip-rap rock along the face of the bank. Since the site is on navigational waters under the authority of the U.S. Army Corps of Engineers, field evaluation of this National Historic Landmark archaeological wet site was required to assess the potential effects of placing protective rip rap rock on the channel bank in order to comply with U.S. Section 106 of the National Historic Preservation Act (Croes et al. 2006)

    Artifact Analyses

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    Over 50 years of collecting and intentional vandalism at the Sunken Village wet site has demonstrated the potential material culture data preservation at this waterlogged site, and finally we can report a systematic characterization of the overall material culture – both debitage and examples of discrete artifacts. The previously and unprofessionally collected basketry, cordage and wooden artifacts reflected the wealth of wood and fiber artifacts thanks to the waterlogged preservation of the site, however collecting is very selective. The analytical power of the basketry was noted over twenty years ago, reflecting a distinct Chinookan style (Croes 1987). As reported next, this artifact analysis may even have a much broader implications for a North Pacific cultural sharing, both in the use of acorn pit processing and distinct basketry styles, factors never perceived before these initial professional explorations

    Ecofacts – Plant and Animal Analyses

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    Wet sites, due to reduced oxygen, provide excellent preservation of both plant and animal remains. Due to the clear importance of Sunken Village as an acorn leaching and processing location – largely because of the aquifer streaming through this approximately 100–125 m of intertidal beach – the focus in Section 4 is on the leaching pits and acorn remains. First we begin with a look at identifying the actual plant materials and fuels used at this site, requiring both visual observations of the plant remains and also cellular analysis of the wood, fibers and charcoal. Many of the perishable artifacts are introduced in this section while identifying their wood and fiber construction materials. Second we report the abundant acorns and acorn leaching pit features, so numerous at Sunken Village. Third, seed retrieval is explored, comparing the results of flotation techniques and fine wet screening. And finally, the faunal analysis reflects the use of these animal resources in a secondary position, and probably to support the group while managing the acorn leaching pits from this site. The obvious contrast here to other Northwest Coast and Columbia River sites is the reduced focus on fisheries from this location

    Early Holocene Occupation at the West Lost River Site, Klamath County, Oregon

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    Excavations at the West Lost River Site (35KL972) provide new insights on early Holocene occupation of southwestern Oregon. The article focuses on the artifacts and specimens recovered from the site

    The effect of organic acid and sodium chloride dips on the shelf-life of refrigerated Irish brown crab (Cancer pagurus) meat

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    peer-reviewedCrab (Cancer pagurus) meat (white and brown) has a short shelf-life. Chemical treatments may inhibit microbial spoilage and extend shelf-life. The effect of 5% organic acids (lactic acid (LA), acetic acid (AA) and citric acid (CA)) and 5% sodium chloride (NaCl) on TVC (mesophiles and psychrophiles), Enterobacteriaceae, Pseudomonas spp. and lactic acid bacteria (LAB) were investigated during storage (2 °C for 12 days). AA was the most effective treatment for white meat, reducing the initial TVCm and TVCp by 1.6 and 1.8 log10 cfu/g, respectively, and extended the shelf life to 8–11.5 days, compared to 5 days for untreated control samples. LA treatment also significantly (P  0.05). A similar pattern was observed for brown meat samples, although the shelf life was increased by a maximum of 1–3 days. The growth of Enterobacteriaceae, Pseudomonas spp. and LAB was significantly (P < 0.05) reduced on AA treated samples only. It was concluded that the shelf-life of crab meat may be extended by up to 3 days using lactic acid and more than doubled using acetic acid.Funding for this project was provided by the Food Institutional Research Measure (FIRM), project number 13F529, administered by the Department of Agriculture, Food and the Marine (DAFM), Ireland
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