The Turkey map showing the location and the number of samples in province level.

<p>The Turkey map showing the location and the number of samples in province level.</p

The working chart of the present study.

<p>1: Optimization; 2: Diagnosis; 3: Species identification.</p

Phylogenetic tree based on the alignment of the amplified section of the ITS1 region of 42 Turkish <i>Leishmania</i> isolates and international reference strains (for <i>L. tropica</i> MHOM/IL/1990/LRC-L590 and MHOM/IL/1996/LRC-L691; for <i>L. major</i> MHOM/IL/2000/LRC-L779; <i>for L. infantum/chagasi</i> MHOM/XX/1999/LRC-L774).

<p>REF*: ITS1 sequences for <i>L. infantum</i> MHOM/TN/1980/IPT1; for <i>L. donovani</i> MHOM/IN/1980/DD8; for <i>L. major</i> MHOM/TM/1973/5ASKH were taken from Talmi-Frank et al. <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002205#pntd.0002205-TalmiFrank2" target="_blank">[47]</a> and included the tree. (VL: visceral isolates; CL: cutaneous isolates; CanL: dog isolates).</p

The type and number of the samples included in the study.

<p>VL: visceral leishmaniasis; CL: cutaneous leishmaniasis; CanL: canine leishmaniasis.</p

The representative figure of two peaks obtained in some samples.

<p>The representative figure of two peaks obtained in some samples.</p