Uji Diagnosis Kesesuian Antara Metode Tes Tinta Terowongan dengan Scraping Kulit terhadap Kasus Scabies pada Kelinci (Lepus domesticus)

This study intend to know the conformity of diagnostic test between burrow ink test method with skin scrapping method of scabies in rabbit. Scabies caused by Sarcoptes scabiei which has a predilection in stratum corneum and lucidum. Clinical symptoms was characterized by papula or crusting of the skin. This study presents a laboratory explorative research with cross sectional approach. Samples used 20 rabbits that infested scabies naturally. Data obtained by performing diagnostic burrow ink test method and skin scrapping in rabbits which are infested scabies naturally. KAPPA test used as data analysis. Results showed that the number of positive results based on burrow ink test method are 12 rabbits and 8 rabbits are negative while skin scrapping obtained the positive results are 11 rabbits and 9 rabbits are negative. KAPPA test analysis performed on the overall study data was obtained 0,490. The conclusion of this study is burrow ink test method has a good conformity with the skin scrapping in rabbits which are infested scabies naturally that can be used for diagnostic analysis of scabies with a simple method, faster, and have the same ability with skin scrapping without hurting animals. Key words : burrow ink test, skin scrapping, scabies, Sarcoptes scabie

DETEKSI ANTIBODI ANTISPERMA PADA SAPI PERAH FRIESIAN HOLSTEIN YANG MENGALAMI KAWIN BERULANG (REPEAT BREEDER) DI KPSP SETIA KAWAN NONGKOJAJAR KECAMATAN TUTUR, PASURUAN PENELITIAN EKSPERIMENTAL LABORATORIS

This study intend to determine the antisperm antibody concentration detected in cervical mucus and blood serum as well as the correlation both of it in repeat breeder Friesian Holstein dairy cows at Koperasi Peternakan Sapi Perah (KPSP) Setia Kawan, Nongkojajar using ELISA-Indirect technique. This study presents a laboratory explorative method by using sample of cervical mucus and blood serum from 11 Friesian Holstein dairy cows, age ≥ 4 years and <9 years (productive age), normal estrus cycle, have given birth before, look healthy and no observed abnormalities. FH dairy cows were divided into 2 groups, consist of 10 dairy cows that have repeat breeder (insemination 3 times or more) and 1 normal dairy cow (insemination 1-2 times) as a control. Samples were examined using indirect-ELISA test method and then the result data were analyzed using Spearman Correlation test in SPSS (Statistical Progams For Social Scientific) program. The results of this study indicate that the antisperm antibody concentration that appears in cervical mucus samples and blood sera of FH dairy cow control (K) has a lower concentration value, that are 0.342 and 54.860 ng/ml. The highest concentration of cervical mucus and blood sera samples was found in FH dairy cows who had repeat breeder with criteria had been mated by artificial insemination (AI) 4 times and not pregnant yet with age around 6.5 years and parity 5 times (P10), each value are 233,776 and 944,531 ng/ml

UJI DIAGNOSIS KESESUAIAN ANTARA METODE TES TINTA TEROWONGAN DENGAN SCRAPPING KULIT TERHADAP KASUS SKABIES PADA KELINCI (Lepus domesticus)

This study intend to know the conformity of diagnostic test between burrow ink test method with skin scrapping method of scabies in rabbit. Scabies caused by Sarcoptes scabiei which has a predilection in stratum corneum and lucidum. Clinical symptoms was characterized by papula or crusting of the skin. This study presents a laboratory explorative research with cross sectional approach. Samples used 20 rabbits that infested scabies naturally. Data obtained by performing diagnostic burrow ink test method and skin scrapping in rabbits which are infested scabies naturally. KAPPA test used as data analysis. Results showed that the number of positive results based on burrow ink test method are 12 rabbits and 8 rabbits are negative while skin scrapping obtained the positive results are 11 rabbits and 9 rabbits are negative. KAPPA test analysis performed on the overall study data was obtained 0,490. The conclusion of this study is burrow ink test method has a good conformity with the skin scrapping in rabbits which are infested scabies naturally that can be used for diagnostic analysis of scabies with a simple method, faster, and have the same ability with skin scrapping without hurting animals

Antisperm Antibody in Repeat Breeder Friesian Holstein Cows at KPSP Setia Kawan Nongkojajar, Tutur District, Pasuruan, Indonesia

This study presents a laboratory explorative method by using sample of cervical mucus and blood serum from 11 Holstein Friesian (HF) cows, aged 4 to 9 years age, normal estrus cycle and gave birth before. They were divided into 2 groups, 10 dairy cows that have history of repeat breeders (4 times or more AI) (RB) and 1 normal breeder (2 times AI) as a control (C). Samples were examined using indirect-ELISA test method. The results of this study indicate that the antisperm antibody concen tration that appears in cervical mucus samples and blood sera of control group has a lower concentration value (0.342 and 54.860 ng/mL) than RB group (233,776 and 944,531 ng/ml)

The Conformity of Diagnostic Test Between Burrow Ink Test Method With Skin Scrapping Method of Scabies in Rabbit (Lepus domesticus)

This study intend to know the conformity of diagnostic test between burrow ink test method with skin scrapping method of scabies in rabbit. Scabies caused by Sarcoptes scabiei which has a predilection in stratum corneum and lucidum. Clinical symptoms was characterized by papula or crusting of the skin. This study presents a laboratory explorative research with cross sectional approach. Samples used 20 rabbits that infested scabies naturally. Data obtained by performing diagnostic burrow ink test method and skin scrapping in rabbits which are infested scabies naturally. KAPPA test used as data analysis. Results showed that the number of positive results based on burrow ink test method are 12 rabbits and 8 rabbits are negative while skin scrapping obtained the positive results are 11 rabbits and 9 rabbits are negative. KAPPA test analysis performed on the overall study data was obtained 0,490. The conclusion of this study is burrow ink test method has a good conformity with the skin scrapping in rabbits which are infested scabies naturally that can be used for diagnostic analysis of scabies with a simple method, faster, and have the same ability with skin scrapping without hurting animals. Keywords: burrow ink test, skin scrapping, scabies, Sarcoptes scabie