48 research outputs found
Specific tagging of the egress-related osmiophilic bodies in the gametocytes of Plasmodium falciparum
<p>Abstract</p> <p>Background</p> <p>Gametocytes, the blood stages responsible for <it>Plasmodium falciparum </it>transmission, contain electron dense organelles, traditionally named osmiophilic bodies, that are believed to be involved in gamete egress from the host cell. In order to provide novel tools in the cellular and molecular studies of osmiophilic body biology, a <it>P. falciparum </it>transgenic line in which these organelles are specifically marked by a reporter protein was produced and characterized.</p> <p>Methodology</p> <p>A <it>P. falciparum </it>transgenic line expressing an 80-residue N-terminal fragment of the osmiophilic body protein Pfg377 fused to the reporter protein DsRed, under the control of <it>pfg377 </it>upstream and downstream regulatory regions, was produced.</p> <p>Results</p> <p>The transgenic fusion protein is expressed at the appropriate time and stage of sexual differentiation and is trafficked to osmiophilic bodies as the endogenous Pfg377 protein. These results indicate that a relatively small N-terminal portion of Pfg377 is sufficient to target the DsRed reporter to the gametocyte osmiophilic bodies.</p> <p>Conclusions</p> <p>This is the first identification of a <it>P. falciparum </it>aminoacid sequence able to mediate trafficking to such organelles. To fluorescently tag such poorly characterized organelles opens novel avenues in cellular and imaging studies on their biogenesis and on their role in gamete egress.</p
Relative Information Gain: Shannon entropy-based measure of the relative structural conservation in RNA alignments
Structural characterization of RNAs is a dynamic field, offeringmanymodelling possibilities. RNA secondary structure models are usually characterized by an encoding that depicts structural information of the molecule through string representations or graphs. In this work, we provide a generalization of the BEAR encoding (a context-aware structural encoding we previously developed) by expanding the set of alignments used for the construction of substitution matrices and then applying it to secondary structure encodings ranging from fine-grained to more coarse-grained representations. We also introduce a re-interpretation of the Shannon Information applied on RNA alignments, proposing a new scoring metric, the Relative Information Gain (RIG). The RIG score is available for any position in an alignment, showing how different levels of detail encoded in the RNA representation can contribute differently to convey structural information. The approaches presented in this study can be used alongside state-ofthe-art tools to synergistically gain insights into the structural elements that RNAs and RNA families are composed of. This additional information could potentially contribute to their improvement or increase the degree of confidence in the secondary structure of families and any set of aligned RNAs
Development of Computational Tools for the Inference of Protein Interaction Specificity Rules and Functional Annotation Using Structural Information
Relatively few protein structures are known, compared to the enormous amount of sequence data produced in the sequencing of different genomes, and relatively few
protein complexes are deposited in the PDB with respect to the great amount of
interaction data coming from high-throughput experiments (two-hybrid or affinity
purification of protein complexes and mass spectrometry). Nevertheless, we can rely
on computational techniques for the extraction of high-quality and information-rich
data from the known structures and for their spreading in the protein sequence space.
We describe here the ongoing research projects in our group: we analyse the protein
complexes stored in the PDB and, for each complex involving one domain belonging
to a family of interaction domains for which some interaction data are available, we
can calculate its probability of interaction with any protein sequence. We analyse the
structures of proteins encoding a function specified in a PROSITE pattern, which
exhibits relatively low selectivity and specificity, and build extended patterns. To
this aim, we consider residues that are well-conserved in the structure, even if their
conservation cannot easily be recognized in the sequence alignment of the proteins
holding the function. We also analyse protein surface regions and, through the
annotation of the solvent-exposed residues, we annotate protein surface patches via a
structural comparison performed with stringent parameters and independently of the
residue order in the sequence. Local surface comparison may also help in identifying
new sequence patterns, which could not be highlighted with other sequence-based
methods
Coding potential of the products of alternative splicing in human
Background: Analysis of the human genome has revealed that as much as an order of magnitude more of the genomic sequence is transcribed than accounted for by the predicted and characterized genes. A number of these transcripts are alternatively spliced forms of known protein coding genes; however, it is becoming clear that many of them do not necessarily correspond to a functional protein. Results: In this study we analyze alternative splicing isoforms of human gene products that are unambiguously identified by mass spectrometry and compare their properties with those of isoforms of the same genes for which no peptide was found in publicly available mass spectrometry datasets. We analyze them in detail for the presence of uninterrupted functional domains, active sites as well as the plausibility of their predicted structure. We report how well each of these strategies and their combination can correctly identify translated isoforms and derive a lower limit for their specificity, that is, their ability to correctly identify non-translated products. Conclusions: The most effective strategy for correctly identifying translated products relies on the conservation of active sites, but it can only be applied to a small fraction of isoforms, while a reasonably high coverage, sensitivity and specificity can be achieved by analyzing the presence of non-truncated functional domains. Combining the latter with an assessment of the plausibility of the modeled structure of the isoform increases both coverage and specificity with a moderate cost in terms of sensitivity
SURFACE: a database of protein surface regions for functional annotation
The SURFACE (SUrface Residues and Functions Annotated, Compared and Evaluated, URL http://cbm.bio.uniroma2.it/surface/) database is a repository of annotated and compared protein surface regions. SURFACE contains the results of a large-scale protein annotation and local structural comparison project. A non-redundant set of protein chains is used to build a database of protein surface patches, defined as putative surface functional sites. Each patch is annotated with sequence and structure-derived information about function or interaction abilities. A new procedure for structure comparison is used to perform an all-versus-all patches comparison. Selection of the results obtained with stringent parameters offers a similarity score that can be used to associate different patches and allows reliable annotation by similarity. Annotation exerted through the comparison of regions of protein surface allows the highlighting of similarities that cannot be recognized by other methods of sequence or structure comparison. A graphic representation of the surface patches, functional annotations and the structural superpositions is available through the web interface
Protein surface similarities: A survey of methods to describe and compare protein surfaces
Many methods have been developed to analyse protein sequences and structures, although less work has been undertaken describing and comparing protein surfaces. Evolution can lead sequences to diverge or structures to change topology; nevertheless, surface determinants that are essential to protein function itself may be mantained. Moreover, different molecules could converge to similar functions by gaining specific surface determinants. In such cases, sequence or structure comparisons are likely to be inadequate in describing or identifying protein functions and evolutionary relationships among proteins. Surface analysis can identify function determinants that are independent of sequence or secondary structure and can therefore be a powerful tool to highlight cases of possible convergent or divergent evolution. This kind of approach can be useful for a better understanding of protein molecular and biochemical mechanisms of catalysis or interaction with a ligand, which are usually surface dependent. Protein surface comparison, when compared to sequence or structure comparison methods, is a hard computational challenge and evaluated methods allowing the comparison of protein surfaces are difficult to find. In this review, we will survey the current knowledge about protein surface similarity and the techniques to detect it
SURFACE: A database of protein surface regions for functional annotation
The SURFACE (SUrface Residues and Functions Annotated, Compared and Evaluated, URL http://cbm.bio.uniroma2.it/surface/) database is a repository of annotated and compared protein surface regions. SURFACE contains the results of a large-scale protein annotation and local structural comparison project. A non-redundant set of protein chains is used to build a database of protein surface patches, defined as putative surface functional sites. Each patch is annotated with sequence and structure-derived information about function or interaction abilities. A new procedure for structure comparison is used to perform an all-versus-all patches comparison. Selection of the results obtained with stringent parameters offers a similarity score that can be used to associate different patches and allows reliable annotation by similarity. Annotation exerted through the comparison of regions of protein surface allows the highlighting of similarities that cannot be recognized by other methods of sequence or structure comparison. A graphic representation of the surface patches, functional annotations and the structural superpositions is available through the web interface