Gene Ontology analysis of genes differentially regulated between haplometrotic and pleometrotic queens.

<p>The figure shows the larger functional groups that encompass significantly enriched GO terms resulting from the GO analysis. To obtain this result we overlapped the list of significantly enriched GO terms from experiment 1 (Functional Annotation Chart, P<0.05) with the GO_slim2 list of the cateGOrizer. Others = larger functional groups encompassing less than 2% of the total number of significantly enriched GO terms (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003633#pgen.1003633.s010" target="_blank">Table S3</a> for the complete list of ancestor GO terms).</p

Expression patterns of aging- and immune-associated genes.

<p>A) Heatmap of log2 transformed and normalized expression values for the fire ant transcripts that are putatively involved in aging processes. This gene list was obtained by overlapping the list differentially regulated transcripts with FlyBase matches in experiment 1 with the list of genes included in the <i>Drosophila</i> GO term “aging”. The directional expression of these transcripts was also compared to the expression patterns of aging genes as found in the review by Paaby and Schmidt <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003633#pgen.1003633-Paaby1" target="_blank">[65]</a>. * = gene that extends lifespan when its activity is increased; ∧ = gene that extends lifespan when its activity is decreased. B) Heatmap of log2 transformed and normalized expression values for the fire ant transcripts that are putatively involved in immune processes. This gene list was obtained by overlapping the list differentially regulated transcripts with FlyBase matches in experiment 1 with the list of canonical immune-related genes annotated in honey bees <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003633#pgen.1003633-Evans1" target="_blank">[48]</a>.</p

Patterns of expression of transcripts associated with lipid metabolic process that were significantly differentially regulated in both experiment 1 and experiment 2 at FDR<0.1.

<p>Patterns of expression of transcripts associated with lipid metabolic process that were significantly differentially regulated in both experiment 1 and experiment 2 at FDR<0.1.</p

Analyses of global gene expression between haplometrotic and pleometrotic queens.

<p>A) Principal Component Analysis of 4080 significantly differentially regulated transcripts. Two PCAs were identified corresponding to the effect of social environment (single queen vs. paired queens, 91.8%) and social rank (winner vs. loser, 8.2%). B) Pairwise comparisons of 4080 significantly differentially regulated transcripts. A total of 3192 transcripts were different between haplometrotic and pleometrotic queens, while only 43 transcripts were different between winner and losers. Haplometrotic queens were more similar to pleometrotic winners than to pleometrotic losers: more transcripts were differentially regulated in haplo vs. los than in haplo vs. win (477 and 404, respectively): this difference was statistically significant (Nominal Logistic Fit: df = 1, ChiSquare = 6.78, P = 0.0092). haplo = haplometrotic queens; los = pleometrotic losers; win = pleometrotic winners.</p

Patterns of expression of the most relevant transcripts that were differentially regulated between winners and losers in experiment 1 (P<0.001).

<p>Patterns of expression of the most relevant transcripts that were differentially regulated between winners and losers in experiment 1 (P<0.001).</p

Main ESM File from Transcriptomics of an extended phenotype: parasite manipulation of wasp social behaviour shifts expression of caste-related genes

Contains supplementary tables, figures, and methods

Graphical representation of the variation in the athletes’ daily stress score ADSS (blue) and budget (orange) over the three periods under study, calculated as the values for each year minus the value of the first year for each period.

<p>The budget for the 2014–2015 season is not yet available.</p

Calculation of the maximal stress score.

<p>The MSS is located on the highest values of the tendency line, as indicated by the red circle.</p

Trends of the main competition load factors over the three periods under study.

<p>Trends of the main competition load factors over the three periods under study.</p

Additional file 1: of The effectiveness of Robot-Assisted Gait Training versus conventional therapy on mobility in severely disabled progressIve MultiplE sclerosis patients (RAGTIME): study protocol for a randomized controlled trial

SPIRIT filled checklist. (DOC 124 kb