P53 expression.

<p>A, B, C and D: invasive cervical cancer epithelium. (A) negative; (B) less than 5% nuclear stain positivity; (C) 5% to 25% nuclear stain positivity; (D) higher than 25% (FastRed, red stain; Mag 40×, Fig A Mag.20×).</p

Association between the patient information with the tissue morphology and the FIGO stage.

<p>Association between the patient information with the tissue morphology and the FIGO stage.</p

Clinical performance of p16^INK4a, Ki-67 and p53 immunostaining in relationship to FIGO stage III or more severe (FIGO III+) and FIGO II+.

<p>Se = sensitivity, Sp = specificity, YI = Youden's Index (Sp+Se−100%).</p>*<p>Highlighted with bold font are the cut point combinations of p16^INK4a and Ki-67 that are most accurate (highest Youden's Index) for consensus diagnosis of FIGO II+.</p

P16^ink4a expression.

<p>A: control epithelium; B, C, D, E and F: invasive cervical cancer epithelium. (A) Negative; (B) Focal moderate nuclear and cytoplasmatic expression; (C) Diffuse weak nuclear and cytoplasmatic expression; (D) Diffuse moderate cytoplasmatic expression; (E) Diffuse strong nuclear and cytoplasmatic expression; (F) Negative (DAB, brown stain; Mag. 40×).</p

Association between p16 expression patterns with the tissue morphology and the FIGO stage.

*<p>Test for trend across ordered groups (np_trend) was used to analyze more than two subgroups for the others were used Fisher's exact.</p

Ki-67 expression.

<p>A, B and C: control epithelium; D, E and F: invasive cervical cancer epithelium. (A) Negative; (B) Strict positive stain in the basal layer; (C) positive stain in the basal and intermediate layer; (D) less than 25% of positive cells all over the epithelium; (E) 25–50% of positive cells all over the epithelium; (F) more than 50% of positive cells all over the epithelium (DAB, brown stain; Mag. 40×).</p

Distribution of human papillomavirus (HPV) genotypes in single and multiple infections.

*<p>HPV DNA positive samples by PCR technique but unable to genotype.</p

Correlation between positive expression of biomarkers and groups of HPV genotypes.

*<p>single and co-infected samples with HPV16.</p

Association between the Ki-67 expression frequencies with the FIGO stage.

<p>Association between the Ki-67 expression frequencies with the FIGO stage.</p