Progress in breeding of irrigated wheat for the Cerrado region of Brazil.

This study aimed to quantify the progress obtained by breeding programs of irrigated wheat in the state of Minas Gerais, Brazil, from 1976 to 2005. The efficiency of the programs was evaluated based on yield data obtained in Value for Cultivation and Use (VCU) trials. The genetic and environmental progress was estimated by the methodology of Vencovsky et al. The mean annual progress rate from 1976 to 2005 in mean yield was estimated to be 48 kg ha-1 yr-1 (1.84% per year). Environmental and technological improvements were important for the increased yield during the study period, accounting for 32.8% of the total gain. Over the years, 33% of the genotypes used in the improvement programs of irrigated wheat were replaced

Procedimentos para viagens a serviço.

Este documento busca orientar, de forma simplificada, os empregados da Embrapa Uva e Vinho sobre os procedimentos obrigatórios em caso de viagem a serviço.bitstream/item/123759/1/FD00117.pd

Integrase-deficient lentiviral vectors mediate efficient gene transfer to human vascular smooth muscle cells with minimal genotoxic risk

We have previously shown that injury-induced neointima formation was rescued by adenoviral-Nogo-B gene delivery. Integrase-competent lentiviral vectors (ICLV) are efficient at gene delivery to vascular cells but present a risk of insertional mutagenesis. Conversely, integrase-deficient lentiviral vectors (IDLV) offer additional benefits through reduced mutagenesis risk, but this has not been evaluated in the context of vascular gene transfer. Here, we have investigated the performance and genetic safety of both counterparts in primary human vascular smooth muscle cells (VSMC) and compared gene transfer efficiency and assessed the genotoxic potential of ICLVs and IDLVs based on their integration frequency and insertional profile in the human genome. Expression of enhanced green fluorescent protein (eGFP) mediated by IDLVs (IDLV-eGFP) demonstrated efficient transgene expression in VSMCs. IDLV gene transfer of Nogo-B mediated efficient overexpression of Nogo-B in VSMCs, leading to phenotypic effects on VSMC migration and proliferation, similar to its ICLV version and unlike its eGFP control and uninfected VSMCs. Large-scale integration site analyses in VSMCs indicated that IDLV-mediated gene transfer gave rise to a very low frequency of genomic integration compared to ICLVs, revealing a close-to-random genomic distribution in VSMCs. This study demonstrates for the first time the potential of IDLVs for safe and efficient vascular gene transfer