116 research outputs found
In vitro template-change PCR to create single crossover libraries: a case study with B. thuringiensis Cry2A toxins
During evolution the creation of single crossover chimeras between duplicated paralogous genes is a known process for increasing diversity. Comparing the properties of homologously recombined chimeras with one or two crossovers is also an efficient strategy for analyzing relationships between sequence variation and function. However, no well-developed in vitro method has been established to create single-crossover libraries. Here we present an in vitro template-change polymerase change reaction that has been developed to enable the production of such libraries. We applied the method to
two closely related toxin genes from B. thuringiensis and created chimeras with differing properties that can help us understand how these toxins are able to differentiate between insect species
Comportamento de híbridos de milho transgênicos e respectivos híbridos isogênicos convencionais em relação à infestação de Spodoptera Frugiperda (j. E. Smith,1727) (Lepidoptera: Noctuidae) em duas localidades e épocas de semeadura
Are Limits of Concern a useful concept to improve the environmental risk assessment of GM plants?
Reply to the EFSA (2016) on the relevance of recent publications (Hofmann et al. 2014, 2016) on environmental risk assessment and management of Bt-maize events (MON810, Bt11 and 1507)
Relação entre toxicidade de proteínas Vip3Aa e sua capacidade de ligação a receptores intestinais de lepidópteros-praga
Genetically modified crops and aquatic ecosystems: considerations for environmental risk assessment and non-target organism testing
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