92 research outputs found
Group B Streptococcus GAPDH Is Released upon Cell Lysis, Associates with Bacterial Surface, and Induces Apoptosis in Murine Macrophages
Glyceraldehyde 3-phosphate dehydrogenases (GAPDH) are cytoplasmic glycolytic enzymes that, despite lacking identifiable secretion signals, have been detected at the surface of several prokaryotic and eukaryotic organisms where they exhibit non-glycolytic functions including adhesion to host components. Group B Streptococcus (GBS) is a human commensal bacterium that has the capacity to cause life-threatening meningitis and septicemia in newborns. Electron microscopy and fluorescence-activated cell sorter (FACS) analysis demonstrated the surface localization of GAPDH in GBS. By addressing the question of GAPDH export to the cell surface of GBS strain NEM316 and isogenic mutant derivatives of our collection, we found that impaired GAPDH presence in the surface and supernatant of GBS was associated with a lower level of bacterial lysis. We also found that following GBS lysis, GAPDH can associate to the surface of many living bacteria. Finally, we provide evidence for a novel function of the secreted GAPDH as an inducer of apoptosis of murine macrophages
A pråtica da não-retenção escolar na narrativa de professores do ensino fundamental: dificuldades e avanços na busca do sucesso escolar
REVISITANDO A NOĂĂO DE JUSTIĂA CURRICULAR: PROBLEMATIZAĂĂES AO PROCESSO DE SELEĂĂO DOS CONHECIMENTOS ESCOLARES
Construção solidĂĄria do habitus escolar: resultados de uma investigação nos setores pĂșblico e privado
Testing With Bars From Dynamic to Quasi-static
International audienceThe numerical calculation of the dynamic loading of a structure includes a great number of steps in which various fundamental or engineering problems are involved. Most of them are addressed in the present course at CISM. In this paper, we discuss the testing of materials in order to model their behaviour
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