Non-Invasive Mapping of the Gastrointestinal Microbiota Identifies Children with Inflammatory Bowel Disease

Background: Pediatric inflammatory bowel disease (IBD) is challenging to diagnose because of the non-specificity of symptoms; an unequivocal diagnosis can only be made using colonoscopy, which clinicians are reluctant to recommend for children. Diagnosis of pediatric IBD is therefore frequently delayed, leading to inappropriate treatment plans and poor outcomes. We investigated the use of 16S rRNA sequencing of fecal samples and new analytical methods to assess differences in the microbiota of children with IBD and other gastrointestinal disorders. Methodology/Principal Findings: We applied synthetic learning in microbial ecology (SLiME) analysis to 16S sequencing data obtained from i) published surveys of microbiota diversity in IBD and ii) fecal samples from 91 children and young adults who were treated in the gastroenterology program of Children’s Hospital (Boston, USA). The developed method accurately distinguished control samples from those of patients with IBD; the area under the receiver-operating-characteristic curve (AUC) value was 0.83 (corresponding to 80.3% sensitivity and 69.7% specificity at a set threshold). The accuracy was maintained among data sets collected by different sampling and sequencing methods. The method identified taxa associated with disease states and distinguished patients with Crohn’s disease from those with ulcerative colitis with reasonable accuracy. The findings were validated using samples from an additional group of 68 patients; the validation test identified patients with IBD with an AUC value of 0.84 (e.g. 92% sensitivity, 58.5% specificity). Conclusions/Significance: Microbiome-based diagnostics can distinguish pediatric patients with IBD from patients with similar symptoms. Although this test can not replace endoscopy and histological examination as diagnostic tools, classification based on microbial diversity is an effective complementary technique for IBD detection in pediatric patients.Natural Sciences and Engineering Research Council of Canada (Award NSERC PGS D)National Institutes of Health (U.S.) (1-R21-A1084032-01A1

Multisite observations of surface structures on AB Doradus in 1994 November

We present time-resolved optical spectroscopy and broad-band photometry of the rapidly rotating southern K0 dwarf star AB Doradus, obtained during 1994 November. The data were obtained as part of a collaboration dedicated to MUlti-SIte COntinuous Spectroscopy (MUSICOS), and entailed coordinated observations on three continents to obtain the fullest phase coverage possible subject to Limitations of local weather conditions. The Doppler images from the three consecutive nights of the run show excellent mutual agreement, with a dark polar cap and numerous intermediate- and low-latitude features. Simultaneous optical photometry showed numerous short-duration U-band flares, and two longer duration optical flares with durations of the order of hours. The latter produced broad-band continuum enhancements throughout the optical spectrum. Where simultaneous spectroscopy was available, both types of flare were seen to have counterparts in H alpha and the Ca II H line. Simultaneous time-resolved ultraviolet spectroscopy from the Goddard High Resolution Spectrograph (GHRS) aboard the Hubble Space Telescope, reported elsewhere, shows that at least one of the short-duration U-band flares was also observed in Cry with the GHRS, Time-series H alpha spectra showed significant evolution of the circumstellar prominence system over five consecutive stellar rotations, One prominence underwent a dramatic increase in distance from the stellar rotation axis. We speculate that this event may have been associated with one of the long-duration flares.</p