2,140 research outputs found

    The Contribution of Public Spaces to Climate Change Adaptation in Austrian Cities

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    Cities and urban areas are particularly affected by the impacts of climate change, especially by rising temperatures and more frequent and heavier rainfall events. Adapting spatial development to the consequences of climate change is being increasingly anchored as a goal in Austrian and European policies and planning strategies. Larger cities are active in setting strategic climate change adaptation (CCA) goals and implementing measures at local scale. Urban green and blue infrastructure (UGBI) and their ecosystem services (ESS) are one of the most effective measures for coping with the consequences of climate change. Urban ecosystems are essential for the urban climate and urban residents due to their regulating (e.g. reduction of the urban heat island effect), supporting (e.g. increase in biodiversity), provisioning (e.g. water filtration) and socio-cultural (e.g. benefits for health, well‐being, recreation) functions. In built-up areas, public space is one of the most important spatial resource for implementing CCA measures and especially UGBI. While steering mechanisms on private properties often fail due to lack of authorisation, instruments or legal regulations, the planning and design of public space is the direct responsibility of cities as local planning authorities. However, in public space, CCA measures and UGBI have to compete with other demands and interests, such as recreation, social interaction and communication, mobility or energy production. As cities grow and densify, pressure on public space increases. Therefore, CCA and CCA measures – both for private and public spaces – need to be mainstreamed into policies at all levels and subsequently integrated into formal planning instruments and planning processes. Our paper investigates the horizontal and vertical integration of CCA, and especially CCA in public space (streets, squares and parks), into Austrian planning-related policies on national, federal state and municipal level and critically reflects on the mechanisms for implementation in planning practice. The research is based on i) a comprehensive analysis of policy documents of four Austrian federal states (Vienna, Styria, Salzburg and Upper Austria) and cities (Vienna, Graz, Salzburg, Wels), and ii) expert interviews with members of federal state and municipal planning administrations. The policy documents include CCA strategies that have an impact on planning and explicit spatial development strategies. The results show a emerging manifestation of CCA in policy documents, though not yet consistently at all levels and in all municipalities. Measures for CCA in public space are mainly anchored at municipal level, as cities already operate actively out of a given urgency. Despite the integration of CCA measures into the strategic planning level, our research shows that implementation still faces obstacles in planning practice. Our study highlights the importance of political agenda setting for the realization of CCA measures in public space and discusses success factors and implementation gaps

    Urban green infrastructure planning as a contribution to the smart ‘green’ city

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    The urban green infrastructure is getting due to the strong growth of the City of Vienna under increasing pressure. A foresighted planning of green and open spaces is necessary to obtain the different "Ecosytem Services" - provision-related services, regulatory services, cultural services and support services (MEA 2005). Additionally an increase in the number of hot days and thus an increase of the heat load in the city is predicted for Vienna (ZAMG 2012). Again, making a foresighted planning of green and open spaces is a significant contribution to meet these climatic challenges (Kuffner A. 2012, Hagen et al. 2010). Based on the concept of "green infrastructure" (Pauleit et al. 2011) and the ecosystem services of these, it is shown which contribution - in particular to reduce the heating of the city - they can make to the Smart City concept

    Possibilities and Opportunities of Mobile Devices to Measure the Physical (In)Activity of Young Citizens – First Results of a Case Study in Vienna

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    “New Media” starting with the introduction of television followed by Video to DVDs and computer games are often made responsible for the lack of movement and outdoor exercises of young people. With the spread of mobile devices such as smartphones and tablet computers, digital tools became spatially independent which offers new options and possibilities especially among young people (Direito et al. 2014). Instead of blaming new media as a reason that the young people increasingly stay at home and neglecting physical activities, the possibilities and opportunities of particularly mobile devices are to be examined in the project “ActivE Youth”. The project aims to determine how mobile devices can contribute for collecting data regarding the mobility behavior of the youth and how mobile devices can be used to reduce the lack of physical activity of young people

    Nutrient availability regulates proline/alanine transporters in Trypanosoma brucei

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    Trypanosoma brucei is a species of unicellular parasite that can cause severe diseases in livestock and humans, including African trypanosomiasis and Chagas disease. Adaptation to diverse environments and changes in nutritional conditions is essential for T. brucei to establish an infection when changing hosts or during invasion of different host tissues. One such adaptation is the ability of T. brucei to rapidly switch its energy metabolism from glucose metabolism in the mammalian blood to proline catabolism in the insect stages and vice versa. However, the mechanisms that support the parasite's response to nutrient availability remain unclear. Using RNAseq and qRT-PCR, we investigated the response of T. brucei to amino acid or glucose starvation and found increased mRNA levels of several amino acid transporters, including all genes of the amino acid transporter AAT7-B subgroup. Functional characterization revealed that AAT7-B members are plasma membrane-localized in T. brucei and when expressed in Saccharomyces cerevisiae supported the uptake of proline, alanine, and cysteine, while other amino acids were poorly recognized. All AAT7-B members showed a preference for proline, which is transported with high or low affinity. RNAimediated AAT7-B downregulation resulted in a reduction of intracellular proline concentrations and growth arrest under low proline availability in cultured procyclic form parasites. Taken together, these results suggest a role of AAT7-B transporters in the response of T. brucei to proline starvation and proline catabolism

    Direct activation of PDE5 by cGMP: long-term effects within NO/cGMP signaling

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    In platelets, the nitric oxide (NO)–induced cGMP response is indicative of a highly regulated interplay of cGMP formation and cGMP degradation. Recently, we showed that within the NO-induced cGMP response in human platelets, activation and phosphorylation of phosphodiesterase type 5 (PDE5) occurred. Here, we identify cyclic GMP-dependent protein kinase I as the kinase responsible for the NO-induced PDE5 phosphorylation. However, we demonstrate that cGMP can directly activate PDE5 without phosphorylation in platelet cytosol, most likely via binding to the regulatory GAF domains. The reversal of activation was slow, and was not completed after 60 min. Phosphorylation enhanced the cGMP-induced activation, allowing it to occur at lower cGMP concentrations. Also, in intact platelets, a sustained NO-induced activation of PDE5 for as long as 60 min was detected. Finally, the long-term desensitization of the cGMP response induced by a low NO concentration reveals the physiological relevance of the PDE5 activation within NO/cGMP signaling. In sum, we suggest NO-induced activation and phosphorylation of PDE5 as the mechanism for a long-lasting negative feedback loop shaping the cGMP response in human platelets in order to adapt to the amount of NO available

    Rapid nitric oxide–induced desensitization of the cGMP response is caused by increased activity of phosphodiesterase type 5 paralleled by phosphorylation of the enzyme

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    Most of the effects of the signaling molecule nitric oxide (NO) are mediated by cGMP, which is synthesized by soluble guanylyl cyclase and degraded by phosphodiesterases. Here we show that in platelets and aortic tissue, NO led to a biphasic response characterized by a tremendous increase in cGMP (up to 100-fold) in less than 30 s and a rapid decline, reflecting the tightly controlled balance of guanylyl cyclase and phosphodiesterase activities. Inverse to the reported increase in sensitivity caused by NO shortage, concentrating NO attenuated the cGMP response in a concentration-dependent manner. We found that guanylyl cyclase remained fully activated during the entire course of the cGMP response; thus, desensitization was not due to a switched off guanylyl cyclase. However, when intact platelets were incubated with NO and then lysed, enhanced activity of phosphodiesterase type 5 was detected in the cytosol. Furthermore, this increase in cGMP degradation is paralleled by the phosphorylation of phosphodiesterase type 5 at Ser-92. Thus, our data suggest that NO-induced desensitization of the cGMP response is caused by the phosphorylation and subsequent activity increase of phosphodiesterase type 5

    KrĂ€uter fĂŒr Nutz- und Heimtiere: Ratgeber fĂŒr die Anwendung ausgewĂ€hlter Heil- und GewĂŒrzpflanzen

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    Dieser Ratgeber fĂŒhrt wissenschaftliche Erkenntnisse und traditionelles Hausmittelwissen zusammen, beleuchtet alles Wissenswerte zu ĂŒber 50 Heilpflanzen und gibt konkrete Anwendungsbeispiele. Ziel ist es, altbewĂ€hrte Pflanzenanwendungen wieder mehr in die moderne Tierhaltung einzubinden. Der anwenderorientierte Aufbau des Buches ermöglicht es dem Leser, Kenntnisse ĂŒber die verschiedenen Zubereitungen und Anwendungen von Heilpflanzen zu erwerben und diese in der Praxis einzusetzen. Zubereitung, Aufbewahrung und Anwendung von KrĂ€utern, sowie deren Wirkung und Einsatz bei einzelnen Tierarten werden ausfĂŒhrlich dargestellt. „Die Aufgabe heutiger Wissenschaft ist weniger die Suche nach neuen wirksamen Pflanzen, vielmehr die ÜberprĂŒfung und Absicherung dieses althergebrachten Wissensschatzes im Lichte moderner Erkenntnisse. Das Autorenteam setzt sich aus jungen engagierten Wissenschaftlern und TierĂ€rzten zusammen. Ihnen ist es ein großes Anliegen, dass die Erkenntnisse der KrĂ€uterheilkunde möglichst vielen Tierhaltern – insbesondere ihren Tieren – von Nutzen sein werden. Es bleibt der Wunsch: die vielen praktischen Anleitungen mögen einen starken Impuls zur Wiederbelebung der KrĂ€uterheilkunde bei Tieren geben.“ Dr. Gerhard Plakol

    Relationship between shear energy input and sedimentation properties of exopolysaccharide-producing StreptococcusStreptococcus thermophilusthermophilus strains

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    separation of the bacteria cells. This separation is most commonly carried out with disc stack separators and needs to be adjusted to the respective strain to obtain a high cell recovery rate. Exopolysaccharides (EPS) produced by several starter cultures, however, have a large negative impact on the separation properties of the cells. These EPS can be divided into cell-bound capsular EPS or free EPS that are released into the surrounding fermentation medium. To improve the separation step, shear forces were applied after fermentation with a gear ring disperser to simulate the impact of a homogenizer and the influence on the separation properties of six Streptococcus thermophilus strains was examined. In case of capsular EPS, the sedimentation velocity of the bacteria increased due to shearing off the capsular EPS layer. Shearing media with free EPS resulted in a viscosity decrease and, hence, in a higher sedimentation velocity, as was determined using a disc centrifuge and a LUMiSizer. Sediment compression as measured with the LUMiSizer was also affected by the shearing step. The results of this study suggest that a defined shear treatment of EPS producing bacterial starter cultures leads to improved separation properties and, hence, higher bacteria yields. We assume that both EPS types affect separation efficiency of the bacteria cells, free EPS because of increased media viscosity and capsular EPS because they act like a friction pad
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