5 research outputs found
Observation of the optical analogue of quantized conductance of a point contact
Wetensch. publicatieFaculteit der Wiskunde en Natuurwetenschappe
A Decomposition Theorem For Maximum Weight Bipartite Matchings with Applications To Evolutionary Trees
Many structures, both man-made and of natural origin, are composed of different elastic materials formed in layers. Often the layers are bonded together along common faces, but it can happen that the bonding is not perfect and flaws occur as cracks or regions of poor bonding in the interface. It is of importance to be able to detect these interface cracks, and one of the most practical methods for accomplishing this, in the cases of engineering interest, utilizes the scattering of elastic waves and the subsequent detection of these scattered waves by appropriate transducers. The goal of this work is to contribute to the theoretical basis for detecting the interface flaw by these means
Gene of DNA-dependent protein kinase catalylic subunit in chronic myeloid leukemia
This study was aimed to investigate the expression and regulation mechanism of DNA-dependent protein kinase catalylic subunit (DNA-PKcs) in chronic myeloid leukemia (CML) and its role in blast crisis of CML. Expression of DNA-PKcs mRNA was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and DNA-PKcs protein by Western blot in 62 CML patients and K562, as compared to those of 23 normal individual controls. In 26 CML patients received allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and 4 CML patients treated with imatinib, the expression of bcr-abl mRNA and DNA-PKcs protein was detected by RT-PCR and Western blot, respectively. After treatment with imatinib in mononuclear cell (MNC) of CML patients and K562 in vitro, expression of DNA-PKcs mRNA was detected by RT-PCR and DNA-PKcs protein level, tyrosine phosphorylation of bcr-abl fusion protein were detected by Western blot. The results showed that the expression of DNA-PKcs protein was significantly lower in CML and K562 than those in normal control (P<0.05). In 26 CML patients received allo-PBSCT and 4 CML patients treated with imatinib, the expression of DNA-PKcs protein was enhanced while the expression of bcr-abl mRNA decreased. After treatment of MNC of CML and K562 with imatinib in vitro, the expression of DNA-PKcs protein was enhanced while tyrosine phosphorylation of bcr-abl fusion protein decreased. It is concluded that the expression of DNA-PKcs protein is down-regulate by bcr-abl fusion gene, and the bcr-abl fusion gene down-regulate the expression of DNA-PKcs protein by post-transcriptional mechanism; the decrease of DNA-PKcs protein expression may be one of mechanisms underlying the acute transformation of CML.link_to_subscribed_fulltex